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Supplementation of Bacillus sp. DU-106 reduces hypercholesterolemia and ameliorates gut dysbiosis in high-fat diet rats

  • Applied microbial and cell physiology
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Abstract

Gut microbiota modulation by a probiotic is a novel therapy for hypercholesterolemia mitigation. This study initially investigated the potential hypocholesterolemic effect of Bacillus sp. DU-106 in hypercholesterolemic rats and explored its potential relation with gut microbiota. Sprague–Dawley rats received a high-fat diet, or a high-fat diet supplemented with 7.5 × 109 and 1.5 × 1010 CFU/kg bw/day Bacillus sp. DU-106 (low-dose and high-dose groups). At the end of 9 weeks, Bacillus sp. DU-106 treatment significantly decreased the body weight, liver index, and total cholesterol. 16S rRNA sequencing showed that Bacillus sp. DU-106 intervention significantly increased bacterial richness and particularly increased the genus abundance of Turicibacter, Acinetobacter, Brevundimonas, and Bacillus and significantly decreased the abundance of Ralstonia. Metabolomic data further indicated that the supplementation of Bacillus sp. DU-106 remarkably changed the gut metabolic profiles of hypercholesterolemic rats and, in particular, elevated the metabolites of indole-3-acetate, methylsuccinic acid, creatine, glutamic acid, threonine, lysine, ascorbic acid, and pyridoxamine. Spearman’s correlation analysis showed the close relation between the different genera and metabolites. In conclusion, Bacillus sp. DU-106 supplement ameliorated high-fat diet-induced hypercholesterolemia and showed potential probiotic benefits for the intestine.

Key points

• A novel potential probiotic Bacillus sp. DU-106 ameliorated hypercholesterolemia in rats.

• Bacillus sp. DU-106 supplement regulated gut microbiome structure and richness.

• Bacillus sp. DU-106 supplement changed metabolic profiles in high-fat diet rats.

• Significant correlations were observed between differential genera and metabolites.

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Acknowledgments

The authors thank the Key Realm R&D Program of Guangdong Province and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams for financial support. We thank Novogene (Beijing, China) for the use of Illumina platform.

Funding

This work was supported by Key Realm R&D Program of Guangdong Province (grant number: 2018B020206001) and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (grant number: 2019KJ125).

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Authors and Affiliations

  1. College of Food Science, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou, 510642, China

    Jianzhao Huang, Nan Xiao, Yiying Sun, Shanshan Wu, Wenni Tian, Yujian Lai, Pan Li & Bing Du

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  1. Jianzhao Huang
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  2. Nan Xiao
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Contributions

Study concept and design: JH, NX, PL, and BD. Conducted the experiments: JH, NX, YS, SW, WT, and YL. Analysis and interpretation of data: JH, NX, YS, PL, and BD. Writing—original draft preparation: JH, NX, YS, and SW. Writing—review and editing: JH, NX, YS, SW, WT, YL, PL, and BD. All authors read and approved the final manuscript.

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Correspondence to Pan Li or Bing Du.

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All animal experiments were performed in compliance with the Chinese legislation on the use and care of laboratory animals and were carried out in Guangzhou Quality Supervision and Testing Institute (Approval No.: SYXK (Yue) 2014-0137). All animal experiments comply with the ARRIVE guidelines.

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Huang, J., Xiao, N., Sun, Y. et al. Supplementation of Bacillus sp. DU-106 reduces hypercholesterolemia and ameliorates gut dysbiosis in high-fat diet rats. Appl Microbiol Biotechnol 105, 287–299 (2021). https://doi.org/10.1007/s00253-020-10977-2

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