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New starch-based radiotracer for lung perfusion scintigraphy

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Abstract

Purpose

In order to avoid the microbiological risks linked to human serum albumin macroaggregates (MAA) used for lung perfusion scintigraphy, we developed a new starch-based Tc-99m potential radiopharmaceutical.

Methods

Microparticles were prepared from oxidised starch coupled to natural polyamine for Tc-99m complexation. Suspensions were formulated as ready-to-use kits for easy one-step labelling procedures.

Results

Particle-size analysis, electron microscopy, and confocal microscopy were performed for microparticle characterisation, and gave a typical size distribution ranging from 7 to 63 µm, with a homogenous population of spherical or oval-shaped microparticles. Radiochemical purity exceeded 95%, and was stable for at least 8 h. When challenged with histidine and human plasma, labelling was also stable. Dynamic scintigraphic acquisitions and biodistribution studies conducted on healthy Wistar rats showed a tracer accumulation with more than 80% of the ID in the lungs after 15 min.

Conclusions

With clinically significant characteristics such as a lung half-life of 3 h, a lung-to-vascular ratio of 900, and a lung-to-liver ratio of 90, starch-based microparticles exhibit all the qualities for an effective new lung perfusion agent.

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Acknowledgements

We are very grateful to E. Porcher for skilful technical support (Inserm U646, University of Angers, F-49100 Angers, France), to Dr. R. Filmon and R. Mallet (SCIAM, University of Angers, F-49100 Angers, France) for microscopy studies.

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Authors and Affiliations

  1. Inserm U646, University of Angers, 10 rue A. Boquel, 49100, Angers, France

    Franck Lacoeuille, François Hindré, Benoit Denizot, Olivier Couturier, Jean Pierre Benoit & Jean Jacques Le Jeune

  2. Nuclear Medicine Department, University Hospital of Angers, 49100, Angers, France

    Franck Lacoeuille, Francis Bouchet, Olivier Couturier & Jean Jacques Le Jeune

  3. SCAHU, UFR Medecine, University of Angers, 49100, Angers, France

    Pierre Legras

  4. Cyclopharma Laboratoires, Biopôle Clermont Limagne, F-63 Saint-Beauzire, France

    Serge Askiénazy

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  1. Franck Lacoeuille
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  2. François Hindré
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  3. Benoit Denizot
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  4. Francis Bouchet
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  6. Olivier Couturier
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  7. Serge Askiénazy
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  8. Jean Pierre Benoit
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  9. Jean Jacques Le Jeune
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Correspondence to Franck Lacoeuille.

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Lacoeuille, F., Hindré, F., Denizot, B. et al. New starch-based radiotracer for lung perfusion scintigraphy. Eur J Nucl Med Mol Imaging 37, 146–155 (2010). https://doi.org/10.1007/s00259-009-1226-6

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  • DOI: https://doi.org/10.1007/s00259-009-1226-6

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