Skip to main content
SpringerLink
Log in

Contribution to the 3D computer assisted reconstruction of pancreatic buds in the rat embryos

  • Original Article
  • Published:
Surgical and Radiologic Anatomy Aims and scope Submit manuscript

Abstract

The aim of this work was to reconstruct, in the rat embryos, stage 12–23, the three dimensional (3D) distribution of the dorsal and ventral pancreatic buds by of a computer assisted method. Ninety-six rat embryos, CRL 3–16 mm, fixed, dehydrated, and paraffin embedded, were submitted to serial histological sections and stained by hematoxylin–eosin and Heidenhain’s azan techniques. The images were digitalized by Canon Camera 350 EOS D. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding. The dorsal pancreas developed from the dorsal wall of the duodenum in stage 12, while the ventral pancreas arose from the ventral wall of the hepatic diverticulum in stage 13 and 14. The rotation of ventral pancreas started in stage 15 and was completed in stage 16. The fusion of both buds was evident in stage 17. In stage 23 the limit between dorsal and ventral bud was still marked by the pathway of superior mesenteric vein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figs. 1-4
Figs. 5-6

Similar content being viewed by others

Notes

  1. Cell image Analyser-A visual scripting interface for Image J and its usage at the microscopy facility Montpellier RIO Imaging Volker Baecker and Pierre Travo in Proceedings of the Image J User and Developer Conference 2006 Ed I p 105–110. ISBN 2-919941-01-1 EAN 978291994018.

References

  1. Abramoff MD, Magelhaes PJ, Ram SJ (2004) Image processing with image. J Biophotonic Int 11(7):36–42

    Google Scholar 

  2. Baecker V, Travo P (2006) Proceedings of the image. J User and developer Conference. Ed I: 105–110, ISBN 2—919941-01-1. EAN: 97 82919941018

  3. Benhidjeb T, Ridwelski K, Wolff H et al (1991) Anomaly of the pancreatico-biliary junction and etiology of choledocal cysts. Zentralbl Chir 116(20):1195–1203

    PubMed  CAS  Google Scholar 

  4. Bossy J (1959) A propos de la persistance des deux ébauches pancréatiques chez l’adulte. Arch Anat 42:119–126

    Google Scholar 

  5. Burdick JS, Horvath E (2006) Management of pancreas divisum. Curr Treat Options Gastroenterol 9(5):391–396

    Article  PubMed  Google Scholar 

  6. Delmas A (1939) Les ébauches pancréatiques dorsales et ventrales; leurs rapports dans la constitution du pancréas définitif. Ann Anat Path 16:253–266

    Google Scholar 

  7. Ferres-Torres R (1968) Estudio morfológico estereometrico del pancreas humano en fases precoces de desarrollo. Ann Anat Granada 17:195–216

    Google Scholar 

  8. Kamisawa T, Tu Y, Egawa N, Tsuruta K et al (2007) MRCP of congenital pancreatico-biliary malformation. Abdom Imaging 32(1):129–133

    Article  PubMed  CAS  Google Scholar 

  9. Maier M, Wiesner W, Mengiardi B (2007) Annular pancreas and agenesis of the dorsal pancreas in a patient with polysplenia syndrome. Am J Roentgenol 188(2):150–153

    Article  Google Scholar 

  10. Ogata M, Chihara N, Matsunobu T et al (2007) Nippon Med Sch. Case of intra-abdominal endocrine tumor possibly arising from an ectopic pancreas. J Nippon Med Sch 74(2):168–172

    Article  PubMed  Google Scholar 

  11. O’Rahilly R, Müller F (1978) A model of the pancreas to illustrate its development. Acta Anat 100:380–385

    Article  PubMed  CAS  Google Scholar 

  12. O’Rahilly R, Müller F (1992) Human embryology and teratology. The digestive system. Chapter 13. Wiley-Liss, pp 170–171

  13. Park HW (1992) Morphologic development of the pancreas in the staged human embryo. Yonsei Med J 33(2):104–108

    PubMed  CAS  Google Scholar 

  14. Prudhomme M, Cristol-Gaubert R, Jeager M, de Reffye P, Godlewski G (1999) A new method of three dimensional computer-assisted reconstruction of the developping biliary tract. Surg Radiol Anat 21:55–58

    Article  PubMed  CAS  Google Scholar 

  15. Regan RA, Wren HB (1962) Annular pancreas. A review with a report of light cases treated surgically. Am J Surg 28:732–740

    CAS  Google Scholar 

  16. Russu IG, Vaida A (1959) A New befunde zur Entwicklung der Bauchspeicheldrüse. Acta Anat 38:114–135

    Article  PubMed  CAS  Google Scholar 

  17. Ueki T, Beppu T, Otan K et al (2006) Three-dimensional computed tomography pancreatography of an annular pancreas with special reference to embryogenesis. Pancreas 32(4):426–429

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Experimental Anatomy, Faculty of Medicine Montpellier-Nimes, University Montpellier 1, Avenue Kennedy, 30908, Nimes, France

    J. Gaubert, R. Cristol-Gaubert, M. Radi, M. Prudhomme, G. Godlewski & D. Prat-Pradal

  2. Montpellier ROI Imaging, CRBM, CNRS, UMR 5237, Route de Mende, 34000, Montpellier, France

    V. Baecker & P. Travo

Authors
  1. J. Gaubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Cristol-Gaubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Radi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Baecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Travo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Prudhomme
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Godlewski
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. D. Prat-Pradal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. Godlewski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gaubert, J., Cristol-Gaubert, R., Radi, M. et al. Contribution to the 3D computer assisted reconstruction of pancreatic buds in the rat embryos. Surg Radiol Anat 31, 31–33 (2009). https://doi.org/10.1007/s00276-008-0394-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00276-008-0394-6

Keywords

Search

Navigation