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Different Photoresponses of Staphylococcus aureus and Pseudomonas aeruginosa to 514, 532, and 633 nm Low Level Lasers In Vitro

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Abstract

Low Level Light irradiation (LLLI) may proliferate cell growth at certain conditions during a set of photochemical reactions called biostimulation. However, phototoxic inhibitory reactions after irradiating natural or artificially inoculated cells are possible. The purpose of this study was to determine these effects on Pseudomonas aeruginosa and Staphylococcus aureus. Ar ion laser at 514 nm was used to determine the effect of various energy densities of green light on these bacteria. The most effective energy densities of Ar ion laser were chosen to irradiate both bacteria with He-Ne (633 nm) and SHG Nd:YAG (532 nm) lasers to compare the effect of red and green lights on the growth. Irradiation of Pseudomonas aeruginosa for both He-Ne and SHG Nd:YAG lasers in the presence of toluidine blue O or safranine O as photosensitizers was also studied. All energy densities of Ar ion laser showed a proliferative effect on Pseudomonas aeruginosa and inhibitory effect on Staphylococcus aureus. Similarly, SHG Nd:YAG and He-Ne lasers with chosen energy densities were again proliferating for Pseudomonas aeruginosa and inhibitory for Staphylococcus aureus and SHG Nd:YAG was more effective than He-Ne in both cases. Irradiation of Pseudomonas aeruginosa in the presence of both photosensitizers led to the decrease of the cell population compared to the control.

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Literature Cited

  1. Bertoloni G, Sacchetto R, Baro E, Ceccherelli F, Jori G (1993) Biochemical and morphological changes in Escherichia coli irradiated by coherent and non-coherent 632.8 nm light. J Photochem Photobiol B Biol 18:191–196

    Article  CAS  Google Scholar 

  2. Boyd R (1988) General microbiology, 2nd edn. Wirtz V (ed.), St. Louis, Missouri: Times Mirror/Mosby College Publishing

  3. DeSimone NA, Christiansen C, Dore D (1999) Bactericidal effect of 0.95 mW Helium-Neon and 5 mW Indium-Gallium-Aluminium-Phosphate laser irradiations at exposure times of 30, 60 and 120 seconds on photosensitized Staphylococcus aureus and Pseudomonas aeruginosa In Vitro. Phys Ther 79:839–845

    PubMed  CAS  Google Scholar 

  4. Giese AC, Hillenkampf F, Pratesi R, Sacchi C (1980) Lasers in biology and medicine. New York: Plenum Press

    Google Scholar 

  5. Kanofsky R (1989) Singlet oxygen production by biological systems. Chem Biol Interact 70:1–28

    Article  PubMed  CAS  Google Scholar 

  6. Karu TI (1988) Molecular mechanism of theraputic effect of low-intensity laser radiation. Lasers Life Sci 2:53–74

    Google Scholar 

  7. Karu TI, Afanasyeva NI, Kolyakov SF, Pyatibrat LV (1998) Changes in absorption spectra of monolayer of living cells after irradiation with low intensity laser light. Dokl Akad Nauk (Moscow) 360(2):267–270

    CAS  Google Scholar 

  8. Karu TI, Afanasyeva NI, Kolyakov SF, Pyatibrat LV, Welser L (2001) Changes in absorbance of monolayer of living cells induced by laser radiation at 633, 670, and 820 nm. IEEE J Sel Top Quantum Electron 7(6):982–988

    Article  CAS  Google Scholar 

  9. Karu TI, Kalendo GS, Letokhov VS (1981) Control of RNA synthesis rate in tumor cells HeLa by action of low-intensity visible light of copper laser. Lett Nuov Cim 32:55–61

    CAS  Google Scholar 

  10. Karu TI, Kalendo GS, Letokhov VS, Lobko VV (1982) Biostimulation of HeLa cells by low intensity visible light. Nuov Cim D 1:828–840

    Article  Google Scholar 

  11. Lee PY, Kim KB, Kim KS (1993) Effects of low incident energy levels of infrared laser irradiation on healing of infected open skin wounds in rats. Laser Ther 5:59–64

    Google Scholar 

  12. Lubart R, Malik Z, Rochkind S, Fisher T (1990) A possible mechanism of low level laser-living cell interaction. Laser Ther 2:65–68

    Google Scholar 

  13. Niemz MH (2002) Laser-tissue interaction: fundamentals and applications. New York: Springer

    Google Scholar 

  14. Nitzan Y, Salmon-Divon M, Shporen E, Malik Z (2004) ALA induced photodynamic effects on gram positive and negative bacteria. Photochem Photobio Sci 3(5):430–435

    Article  CAS  Google Scholar 

  15. Tiphlova O, Karu TI (1991) Action of low-intensity laser radiation on Escherichia coli. CRC Critical Rev Biomed Eng 18:387–394

    CAS  Google Scholar 

  16. Vo-Dinh T (2003) Biomedical Photonics Handbook. New York: CRC Press

    Google Scholar 

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Acknowledgments

Financial support from “Ministry of Industries and Mines High Tech Industries Centre” is gratefully acknowledged.

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Authors and Affiliations

  1. Laser Research Institute, Shahid Beheshti University, Tehran, Iran

    Siamak Dadras & Ezzeddin Mohajerani

  2. Department of Biology, Shahid Beheshti University, Tehran, Iran

    Fereshteh Eftekhar & Masoud Hosseini

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  1. Siamak Dadras
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  2. Ezzeddin Mohajerani
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  3. Fereshteh Eftekhar
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  4. Masoud Hosseini
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Correspondence to Ezzeddin Mohajerani.

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Dadras, S., Mohajerani, E., Eftekhar, F. et al. Different Photoresponses of Staphylococcus aureus and Pseudomonas aeruginosa to 514, 532, and 633 nm Low Level Lasers In Vitro. Curr Microbiol 53, 282–286 (2006). https://doi.org/10.1007/s00284-005-0490-3

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  • DOI: https://doi.org/10.1007/s00284-005-0490-3

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