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Relative Expression of Low Molecular Weight Protein, Tyrosine Phosphatase (Wzb Gene) of Herbaspirillum sp. GW103 Toward Arsenic Stress and Molecular Modeling

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Abstract

This study investigated the expression rate and molecular modeling of Wzb gene, a low molecular weight protein tyrosine phosphatase, under As stress in Herbaspirillum sp. GW103. Expression of Wzb gene was quantified at transcriptional level through real-time quantitative PCR. The results showed up- and down-regulations of Wzb gene in the presence of As (50 and 100 mg/L). The maximum Wzb transcript expression was 1.2-fold after 72 h exposure to 50 mg/L of As. However, the minimum expression was 0.1-fold after 48 h exposure to 100 mg/L of As. The Wzb protein sequence was retrieved from NCBI sequence database and was used for in silico analysis. 3D structure of Wzb gene was predicted by comparative modeling using modeler 9v9. Further, the model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D, and SAVES server which revealed structure and quality of the Wzb gene model.

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Acknowledgments

This research work was supported by the National Research Foundation of Korea (NRF) Grant funded by the government (MEST; No. 2011-0020202).

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    Authors and Affiliations

    1. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570-752, South Korea

      Muthusamy Govarthanan, Jung-Hee Park, Loganathan Praburaman, Young-Joo Yi, Min Cho, Seralathan Kamala-Kannan & Byung-Taek Oh

    2. Department of Ecology Landscape Architecture - Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570-752, South Korea

      Hyun Myung

    3. PG & Research Department of Biotechnology, Mahendra Arts & Science College, Kalippatti, 637 501, TamilNadu, India

      Shanmugam Gnanendra

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    1. Muthusamy Govarthanan
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    4. Young-Joo Yi
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    Correspondence to Seralathan Kamala-Kannan or Byung-Taek Oh.

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    Muthusamy Govarthanan and Jung-Hee Park have contributed equally to this work.

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    Govarthanan, M., Park, JH., Praburaman, L. et al. Relative Expression of Low Molecular Weight Protein, Tyrosine Phosphatase (Wzb Gene) of Herbaspirillum sp. GW103 Toward Arsenic Stress and Molecular Modeling. Curr Microbiol 71, 311–316 (2015). https://doi.org/10.1007/s00284-015-0850-6

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    • DOI: https://doi.org/10.1007/s00284-015-0850-6

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