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Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing

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Abstract

Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) and solid-state fermentation (SSF). Under SmF and SSF, 0.60 g l−1 and 1.56 g l−1 of PHB with 0.497 g l−1 of yellow fluorescent pigment (YFP) was produced. Fourier transform infrared (FTIR) absorption bands at 1719–1720 cm−1 indicate the presence of C=O group of PHB. Nuclear magnetic resonance (NMR) exhibited the typical chemical shift patterns of PHB, and crystallinity was confirmed from X-ray diffraction (XRD). The melting temperature (Tm), degradation temperature (Td) and crystallinity (Xc) of extracted PHB were found to be 171 °C, 288 °C and 35%, respectively. FACS (Fluorescence-activated cell sorting) confirmed cytocompatibility of PHB at 400 µg ml−1 in mouse fibroblast line. Moreover, biodegradability and elevated cytocompatibility of the PHB produced through SSF make them highly potential biomaterials to be used as a drug delivery carrier in future.

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Authors and Affiliations

  1. Department of Microbiology, CBSH, OUAT, Bhubaneswar-3, Odisha, India

    Swayamsidha Pati, Ankita Dash & Devi P. Samantaray

  2. University Innovation Cluster-Biotechnology, CCT, University of Rajasthan, Jaipur, Rajasthan, India

    Sudipta Maity

  3. R & D Division, IMGENEX India Private Limited, Bhubaneswar, Odisha, India

    Soubhagyalaxmi Jema

  4. Department of Microbial Technology, AMITY University, Noida, New Delhi, India

    Swati Mohapatra

  5. Department of Life Science, NIT, Rourkela, Odisha, India

    Surajit Das

Authors
  1. Swayamsidha Pati
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  2. Sudipta Maity
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  3. Ankita Dash
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  4. Soubhagyalaxmi Jema
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  5. Swati Mohapatra
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  7. Devi P. Samantaray
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Correspondence to Devi P. Samantaray.

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Pati, S., Maity, S., Dash, A. et al. Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing. Curr Microbiol 77, 1203–1209 (2020). https://doi.org/10.1007/s00284-020-01922-7

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  • DOI: https://doi.org/10.1007/s00284-020-01922-7

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