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Prevalence of Campylobacter spp. in Chicken Carcasses in Slaughterhouses from South of Brazil

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Abstract

Campylobacteriosis has become common cause of diarrhea in humans and is associated with Guillain–Barré Syndrome, Reactive Arthritis and Irritable Bowel Syndrome is caused mainly by contaminated food and water intake in which the majority occurs from manipulation, preparation and consumption of poultry meat. The aim of this study was to estimate the prevalence of Campylobacter in chicken carcasses from slaughterhouses located in the states of Parana, Santa Catarina and Rio Grande do Sul in the South of Brazil. The samples were analyzed for Campylobacter enumeration using the ISO method 10272-2 and the species C. jejuni and C. coli, important for public health, were identified through Maldi-TOF mass spectrometry. From July 2017 to July 2018, 816 samples were analyzed, indicating the prevalence of 35.84%, with higher occurrence of C. jejuni (78.47%). No difference in prevalence was observed in relation to the size of the slaughterhouses. However, significant differences were noted among the three states in the southern region of the country, with the lowest prevalence being observed in Parana. The results reinforce the need to advance in the implementation of strategies to control this pathogen in the country, in order to safeguard consumer’s health and contribute for the maintenance of Brazil’s position in the international poultry meat market.

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References

  1. Wagenaar JA, French NP, Havelaar AH (2013) Preventing Campylobacter at the source: why is it so difficult? Clin Infect Dis 57:1600–1606

    Article  Google Scholar 

  2. WHO/FAO World Health Organization/Food and Agriculture Organization (2013) The global view of campylobacteriosis: report of an expert consultation. Utrecht

  3. Pitkanen T, Hanninen ML (2017) Members of the family Campylobacteraceae: Campylobacter jejuni, Campylobacter coli. In: Rose JB, Jiménez-Cisneros B (eds) Global water pathogens project. Michigan State University, E. Lansing, MI, UNESCO

  4. EFSA European Food Safety Authority and European Centre For Disease Prevention and Control (ECDC) (2018) The European union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J 16:5077

    Google Scholar 

  5. Epps SVR, Harvey RB, Hume ME et al (2013) Review foodborne Campylobacter: infections, metabolism, pathogenesis and reservoirs. Int J Environ Res Public Health 10:6292–6304

    Article  Google Scholar 

  6. Hoffmann S, Devleesschauwer B, Aspinall W et al (2017) Attribution of global foodborne disease to specific foods: findings from a World Health Organization structured expert elicitation. PLoS ONE 12:e0183641

    Article  Google Scholar 

  7. USDA/FSIS United States Department of Agriculture, Food Safety and Inspection Service (2008) The nationwide microbiological baseline data collection program: young chicken survey. July 2007–2008

  8. Canada (2016) Canadian Food Inspection Agency (CFIA). National microbiological baseline stud y in broiler chicken. December 2012–2013

  9. EFSA European Food Safety Authority (2010) Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008, Part A: Campylobacter and Salmonella prevalence estimates. EFSA J 8:1503

    Google Scholar 

  10. European Union (EU) (2017) Commission regulation (EC) 2017/1495 of 23 August 2017 amending regulation (EU) 2073/2005 as regards Campylobacter in broiler carcasses. Official Journal of European Union

  11. Brasil (2017) Ministério da Agricultura, Pecuária e Abastecimento. Norma Interna DIPOA/SDA n 2, de 12 de junho de 2017. Estabelece o programa exploratório para pesquisa e estimativa de Campylobacter spp em carcaças de frangos. Boletim de Pessoal, Brasília

  12. ISO International Standards Organization (2017) 17025 General requirements for the competence of testing and calibration laboratories. International Standard ISO, Geneva

    Google Scholar 

  13. ISO International Standards Organization (2017) 10272-2 Microbiology of the food chain—horizontal method for the detection and enumeration of Campylobacter spp. Part 2: Colony count technique. International Standard—ISO. Geneva

  14. Rodrigues CS, Armendaris PM (2018) Avaliação de amostras de carcaças de frango para presença e prevalência de Campylobacter nos SIFs do Brasil. Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA). http://www.facta.org.br/salmonella2018/anais/view/home/. Accessed Apr 17 2019

  15. StataCorp (2015) Stata statistical software: release 14. College Station, Texas

    Google Scholar 

  16. Rodrigues WOP, Garcia RG, Naas IA et al (2014) Evolução da avicultura de corte no Brasil. Enciclopédia Biosfera, Centro Científico Conhecer 10:1666–1684

    Google Scholar 

  17. Sheppard SK, Maiden MCJ (2015) The evolution of Campylobacter jejuni and Campylobacter coli. Cold Spring Harb Perspect Biol 7:a018119

    Article  Google Scholar 

  18. Seliwiorstow T, Zutter L, Houf K et al (2016) Comparative performance of isolation methods using Preston broth, Bolton broth and their modifications for the detection of Campylobacter spp from naturally contaminated fresh and frozen raw poultry meat. Int J Food Microbiol 234:60–64

    Article  CAS  Google Scholar 

  19. Melo RT (2012) Fatores de patogenicidade e potencial risco à saúde em Campylobacter spp. isoladas em carcaças de frango. Dissertation, Federal University of Uberaba

  20. Lobo A (2018) Desempenho logístico: Brasil sofre com falta de infraestrutura. ILOS Especialistas em Logística e Supply Chain. http://www.ilos.com.br/web/lpi-o-brasil-continua-sofrendo-com-a-infraestrutura/. Accessed May 14 2019

  21. EFSA European Food Safety Authority (2010) Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008, Part B: Analysis of factors associated with Campylobacter colonization of broiler batches and with Campylobacter contamination of broiler carcasses; and investigation of the culture method diagnostic characteristics used to analyse broiler carcass samples. EFSA J 8:1522

    Article  Google Scholar 

  22. Lee J, Castle M, Duncan G et al (2015) Example of a microbiological criterion (MC) for verifying the performance of a food safety control system: Campylobacter performance target at end of processing of broiler chickens. Food Control 58:23–28

    Article  CAS  Google Scholar 

  23. Sampers I, Habib I, Zutter L et al (2010) Survival of Campylobacter spp in poultry meat preparations subject to freezing, refrigeration, minor salt concentration and heat treatment. Int J Food Microbiol 137:147–153

    Article  CAS  Google Scholar 

  24. Rosenquist H, Sommer HM, Nielsen NL et al (2006) The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int J Food Microbiol 108:226–232

    Article  Google Scholar 

  25. Sandberg M, Hofshagen M, Ostensvik O et al (2005) Survival of Campylobacter on frozen broiler carcasses as a function of time. J Food Prot 68:1600–1605

    Article  Google Scholar 

  26. WHO/FAO World Health Organization/Food and Agriculture Organization (2009) Risk assessment of Campylobacter spp. in broiler chickens: interpretative summary. Microbiological Risk Assessment Series No 11. Geneva

  27. Han Z, Willer T, Li L et al (2017) Influence of the gut microbiota composition on Campylobacter jejuni colonization in chickens. Infect Immun 85:e00380-e417

    Article  CAS  Google Scholar 

  28. Melo RT, Grazziotin AL, Júnior-Valadares EC et al (2019) Evolution of Campylobacter jejuni of poultry origin in Brazil. Food Microbiol 82:489–496

    Article  CAS  Google Scholar 

  29. Berghaus RD, Thayer SG, Law BF et al (2013) Enumeration of Salmonella and Campylobacter spp in environmental farm samples and processing plant carcass rinses from commercial broiler chicken flocks. Appl Environ Microbiol 79:4106–4114

    Article  CAS  Google Scholar 

  30. Nauta M, Johannessen G, Adame LL et al (2016) The effect of reducing numbers of Campylobacter in broiler intestines on human health risk. Microb Risk Anal 1–2:68–77

    Article  Google Scholar 

  31. CAC Codex Alimentarius Commission (2011) Guidelines for the control of Campylobacter and Salmonella in chicken meat. CAC/GL 78/2011

  32. Seliwiorstow T, Baré J, Berkvens D et al (2016) Identification of risk factors for Campylobacter contamination levels on broiler carcasses during the slaughter process. Int J Food Microbiol 226:26–32

    Article  Google Scholar 

  33. Lehner Y, Reich F, Klein G (2014) Influence of process parameter on Campylobacter spp. counts on poultry meat in a slaughterhouse environment. Curr Microbiol 69:240–244. https://doi.org/10.1007/s00284-014-0575-y

    Article  CAS  PubMed  Google Scholar 

  34. Kouglenou SD, Agbankpe AJ, Dougnon V, Djeuda AD, Deguenon E, Hidjo M, Baba-Moussa L, Bankole H (2020) Prevalence and susceptibility to antibiotics from Campylobacter jejuni and Campylobacter coli isolated from chicken meat in southern Benin. West Africa BMC Res Notes 13:305. https://doi.org/10.1186/s13104-020-05150-x

    Article  CAS  PubMed  Google Scholar 

  35. Ramonaitė S, Novoslavskij A, Zakarienė G, Aksomaitienė J, Malakauskas M (2015) High prevalence and genetic diversity of Campylobacter jejuni in wild crows and pigeons. Curr Microbiol 71:559–565. https://doi.org/10.1007/s00284-015-0881-z

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

To Coordination for the Improvement of Higher Education Personnel (CAPES) and to the Ministry of Agriculture, Livestock and Food Supply (MAPA) (Process No. 21000.055750/2018-26). The author CB de Melo is a Research Productivity Fellow (PQ) from the National Council for Scientific and Technological Development—Brazil (CNPq).

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Authors and Affiliations

  1. Infectious Diseases of Mandatory Notification Laboratory, University of Brasília, ICC Sul, Campus Darcy Ribeiro, Brasília, DF, 70910-900, Brazil

    Carla Susana Rodrigues & Cristiano Barros de Melo

  2. Department of Inspection of Animal Products, Ministry of Agriculture, Livestock and Food Supply, Secretariat of Animal and Plant Health and Inspection, Brasília, DF, Brazil

    Carla Susana Rodrigues

  3. Federal Laboratory of Agricultural Defense, Ministry of Agriculture, Livestock and Food Supply, Secretariat of Animal and Plant Health and Inspection, Porto Alegre, Brazil

    Paulo Marcel Armendaris

  4. Department of Support and Standards, Ministry of Agriculture, Livestock and Food Supply, Secretariat of Animal and Plant Health and Inspection, Brasília, Brazil

    Claudia Valéria Gonçalves Cordeiro de Sá

  5. Department of Preventive Veterinary Medicine and Epidemiology, Federal University of Minas Gerais, Veterinary College, Belo Horizonte, Brazil

    João Paulo Amaral Haddad

Authors
  1. Carla Susana Rodrigues
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  2. Paulo Marcel Armendaris
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  3. Claudia Valéria Gonçalves Cordeiro de Sá
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  5. Cristiano Barros de Melo
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The authors contributed equally to the manuscript.

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Correspondence to Cristiano Barros de Melo.

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Rodrigues, C.S., Armendaris, P.M., de Sá, C.V.G.C. et al. Prevalence of Campylobacter spp. in Chicken Carcasses in Slaughterhouses from South of Brazil. Curr Microbiol 78, 2242–2250 (2021). https://doi.org/10.1007/s00284-021-02478-w

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