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Improved infrared spectroscopic method for the analysis of degree of N-deacetylation of chitosan

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Summary

Various infrared spectroscopic techniques for the analysis of degree of N-deacetylation of chitosan were evaluated for accuracy. A new method was proposed which involved development of a calibration curve using the absorbance intensities of the chitosan infrared spectrum at 1655 and 3450 cm−1. The degree of N-deacetylation of various chitosan samples was then determined by using the absorption ratios [A1655/A3450] in the equation of the calibration curve. This method yielded results which were superior to those of any of the previously reported infrared spectroscopic methods tested herein.

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Authors and Affiliations

  1. Research and Technology, Block Drag Company, Inc., 257 Cornelison Avenue, 07302, Jersey City, NJ, USA

    Shobhan Sabnis

  2. Department of Medicinal Chemistry and Pharmaceutics, Graduate School of Pharmaceutical Sciences, Duquesne University, 15282, Pittsburgh, PA, USA

    Lawrence H. Block

Authors
  1. Shobhan Sabnis
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  2. Lawrence H. Block
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Sabnis, S., Block, L.H. Improved infrared spectroscopic method for the analysis of degree of N-deacetylation of chitosan. Polymer Bulletin 39, 67–71 (1997). https://doi.org/10.1007/s002890050121

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  • DOI: https://doi.org/10.1007/s002890050121

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