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Regeneration of the Egyptian medicinal plant Artemisia judaica L.

  • Cell Biology and Morphogenesis
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Abstract

An in vitro propagation system for Artemisia judaica L., a traditional Egyptian medicinal plant, has been developed. De novo shoot organogenesis was induced by culturing etiolated hypocotyls and intact seedlings on medium supplemented with thidiazuron [N-phenyl-N′-(1,2,3-thidiazol-yl) urea] via callusing at the cotyledonary notch region. Up to 16 shoots formed per seedling cultured on a medium containing 1 µmol l−1 thidiazuron for an optimal duration of exposure of 20 days. Regenerated shoots formed roots when subcultured onto a medium containing 1 µmol l−1 indole-3-butyric acid. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. judaica.

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Fig. 1A–F.
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Fig. 4A,
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Abbreviations

BAP :

Benzyladenine

TDZ :

Thidiazuron

IBA :

Indole-3-butyric acid

NAA :

α-Naphthaleneacetic acid

PGRs :

Plant growth regulators

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Acknowledgements

The authors are grateful for the collaboration and support of staff of the St. Katherine Protectorate, Sinai, Egypt. The financial support of the Natural Sciences and Engineering Research Council of Canada is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Agriculture, Biotechnology Division, Edmund C. Bovey Complex, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    C. Z. Liu, S. J. Murch & P. K. Saxena

  2. Faculty of Science, University of Mansoura, Mansoura, Egypt

    M. EL-Demerdash

Authors
  1. C. Z. Liu
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  2. S. J. Murch
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  3. M. EL-Demerdash
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  4. P. K. Saxena
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Corresponding author

Correspondence to P. K. Saxena.

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Communicated by L.C. Fowke

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Liu, C.Z., Murch, S.J., EL-Demerdash, M. et al. Regeneration of the Egyptian medicinal plant Artemisia judaica L.. Plant Cell Rep 21, 525–530 (2003). https://doi.org/10.1007/s00299-002-0561-x

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  • DOI: https://doi.org/10.1007/s00299-002-0561-x

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