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In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya

  • Cell Biology and Morphogenesis
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Abstract

This is the first report of a micropropagation protocol for Saussurea obvallata (DC.) Edgew. (Asteraceae), a rare, threatened and near-endemic medicinal herb of the Indian Himalayan region. Multiple shoots were formed from epicotyle explants on Murashige and Skoog (MS) medium supplemented with 1.0 µM kinetin and 0.25 µM α-naphthaleneacetic acid. A maximum of five shoots were obtained from one explant in a 75-day culture period. The effect of subsequent subcultures on shoot formation was also studied. After 100% in vitro rooting was obtained in half-strength MS supplemented with 2.5 µM Indole-3-butyric acid, the plantlets were transferred to ex vitro conditions. Following a 15-day in vitro rooting period and 12 days of ex vitro acclimatization, 66.7% of the plantlets had established in the field. Application of this protocol has the potential to substantially reduce the pressure on natural populations

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Fig. 1.
Fig. 2A–D.
Fig. 3.

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Abbreviations

BA :

6-Benzyladenine

IBA :

Indole-3-butyric acid

Kin :

Kinetin

NAA :

α-Naphthaleneacetic acid

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Acknowledgements

The authors are grateful to the Director, G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora for encouragement. MJ wishes to thank Drs. R.S. Rawal, S. Airi, I.D. Bhatt and colleagues of the CBD group for their support and help. The authors also thank the anonymous reviewers for their valuable comments on the earlier version of this manuscript. Financial assistance from the Department of Biotechnology, Government of India (BT/PR1118/PB/17/050/98) is gratefully acknowledged.

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  1. G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, 263643, Almora, (UA), India

    M. Joshi & U. Dhar

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  1. M. Joshi
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  2. U. Dhar
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Correspondence to U. Dhar.

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Communicated by H. Lörz

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Joshi, M., Dhar, U. In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep 21, 933–939 (2003). https://doi.org/10.1007/s00299-003-0601-1

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  • DOI: https://doi.org/10.1007/s00299-003-0601-1

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