Abstract
We have investigated the possible relation between plant cell-wall constituents and the recalcitrance of the cell to regenerate organs and whole plants in vitro. A temporal and spatial expression of several carbohydrate epitopes was observed both within leaf tissue used for protoplast isolation and within new walls reformed by recalcitrant mesophyll protoplasts of sugar beet (Beta vulgaris L.); these include four pectic epitopes, one xyloglucan (rhamnogalacturonan I) epitope, two carbohydrate motifs of arabinogalactan proteins (AGPs) and callose. The walls of mesophyll cells and newly formed walls of protoplasts were similar with respect to the presence of large amounts of pectins recognized by JIM7 antibodies, the scarcity of JIM5-pectins and the complete absence of LM5-responding pectin molecules. Their main differences were the significantly higher accumulation of LM6-recognizing pectins and the very conspicuous greater accumulation of AGPs and callose in walls deposited by protoplasts than in those synthesized by donor cells.
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Abbreviations
- AGPs :
-
Arabinogalactan proteins
- BSA :
-
Bovine serum albumin
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Acknowledgements
This study was supported by The Polish Committee for Scientific Research (project no. 5-P06A 040-17). The authors thank Dr. K. Roberts (John Innes Centre, UK) for providing the JIM5, JIM7 and JIM13 antibodies, Dr. P. Knox (University of Leeds, UK) for the LM2 antibody, and Dr. M. Hahn (University of Georgia, USA) for the CCRC-M1 antibody. We acknowledge M.I. Rodriguez-Garcia (Estación Experimental del Zaidin, CSIC, Spain) and A. Tretyn (University of Nicolas Copernicus, Poland) for the use of electron microscopes. We also thank K. Shashok and Sz. Andrzejewski for correcting the English version of the manuscript.
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Majewska-Sawka, A., Münster, A. Cell-wall antigens in mesophyll cells and mesophyll-derived protoplasts of sugar beet: possible implication in protoplast recalcitrance?. Plant Cell Rep 21, 946–954 (2003). https://doi.org/10.1007/s00299-003-0612-y
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DOI: https://doi.org/10.1007/s00299-003-0612-y