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Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]

  • Genetic Transformation and Hybridization
  • Published:
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Abstract

Modern genetic analysis and manipulation of soybean (Glycine max) depend heavily on an efficient and dependable transformation process, especially in public genotypes from which expressed sequence tag (EST), bacterial artificial chromosome and microarray data have been derived. Williams 82 is the subject of EST and functional genomics analyses. However, it has not previously been transformed successfully using either somatic embryogenesis-based or cotyledonary-node transformation methods, the two predominant soybean transformation systems. An advance has recently been made in using antioxidants to enhance Agrobacterium infection of soybean. Nonetheless, an undesirable effect of using these antioxidants is the compromised recovery of transgenic soybean when combined with the use of the herbicide glufosinate as a selective agent. Therefore, we optimized both Agrobacterium infection and glufosinate selection in the presence of l-cysteine for Williams 82. We have recovered transgenic lines of this genotype with an enhanced transformation efficiency using this herbicide selection system.

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Fig. 1a–c

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Abbreviations

DTT :

Dithiothreitol

EST :

Expressed sequence tag

GUS :

β-Glucuronidase

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Acknowledgements

We would like to thank Jamie Schlereth and Lan Wang for their technical assistance. Special thanks to Dr. Hari Krishnan for critical review of this manuscript. This research is supported by grants #9872565 from the NSF Plant Genome Initiative and from the University of Missouri-Columbia Life Sciences Mission Enhancement Program. All experiments were conducted at the Plant Transformation Core Facility, University of Missouri—Columbia.

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Authors and Affiliations

  1. Department of Biochemistry, University of Missouri-Columbia, 111 Schweitzer Hall , Columbia, MO 65211, USA

    P. Zeng & J. C. Polacco

  2. Plant Transformation Core Facility and Department of Agronomy, University of Missouri-Columbia, 1–87 Agriculture Building , Columbia, MO 65211, USA

    D. A. Vadnais & Z. Zhang

Authors
  1. P. Zeng
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  2. D. A. Vadnais
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  3. Z. Zhang
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  4. J. C. Polacco
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Correspondence to Z. Zhang.

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Communicated by P. Ozias-Akins

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Zeng, P., Vadnais, D.A., Zhang, Z. et al. Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 22, 478–482 (2004). https://doi.org/10.1007/s00299-003-0712-8

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  • DOI: https://doi.org/10.1007/s00299-003-0712-8

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