Abstract
Somatic embryogenesis from mature elm (Ulmus minor Mill.) in vitro-cloned material is possible. Embryogenic callus was obtained from leaves inoculated on two different MS-based media—one supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid (I2) and the other supplemented with 1.1 μM kinetin (I6). However, only leaves cultured on medium I6 produced somatic embryos, at the globular stage, when embryogenic callus was maintained in induction media. When embryogenic callus from medium I6 was transferred to basal medium, somatic embryos with green cotyledons were obtained. An average of 35.9% of these embryos converted easily into normal plants in conversion medium with 1% sucrose. Acclimatisation reached 39.7%, and this was not significantly different from a control group consisting of plants propagated by axillary buds. No morphological differences were observed between plants derived from somatic embryos and control plants. Also, no differences in ploidy were detected between the somatic embryo-derived plants and the mother plants.
Similar content being viewed by others
Abbreviations
- BA::
-
Benzyladenine
- C1, C2::
-
Conversion media
- 2,4-D::
-
2,4-Dichlorophenoxyacetic acid
- Kn::
-
Kinetin
- NAA::
-
α-Naphthaleneacetic acid
- PI::
-
Propidium iodide
- I2, I6::
-
Induction media
References
Ammirato PV (1988) Role of ABA in regulation of somatic embryogenesis. HortScience 23:520
Ben Jouira H, Hassairi A, Bigot C, Dorion N (1998) Adventitious shoot production from strips of stem in the Dutch Elm hybrid “Commelin”: plantlet regeneration and neomycin sensitivity. Plant Cell Tissue Organ Cult 53:153–160
Bolyard MG, Srinivasan C, Cheng J, Sticklen MB (1991) Shoot regeneration from leaf explants of American and Chinese Elm. Hortscience 26:1554–1555
Cheng ZM, Shi NQ (1995) Micropropagation of mature Siberian Elm in two steps. Plant Cell Tissue Organ Cult 41:197–199
Corredoira E, Vieitez AM, Ballester A (2002) Somatic embryogenesis in Elm. Ann Bot 89:637–644
Dorion N, Danthu P, Bigot C (1987) Multiplication végétative in vitro de quelques espèces d’Ormes. Ann Sci For 44:103–118
Dorion N, Ben Jouira H, Danthu P, Bigot C (1994) Regeneration of plants from protoplasts of Ulmus species (elms). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 29. Plant protoplasts and genetic engineering V. Springer, Berlin Heidelberg New York, pp 172–189
Dunn CP (2000) The elms—breeding, conservation and disease management. Kluwer, Dordrecht
Durzan DJ, Lopushansky SM (1975) Propagation of American Elm via cell suspension cultures. Can J For Res 5:273–277
Fenning TM, Gartland KMA, Brasier CM (1993) Micropropagation and regeneration of English Elm, Ulmus procera Salisbury. J Exp Bot 44:1211–1217
Fenning TM, Tymens SS, Brasier CM, Gartland JS, Gartland KMA (1996a) A strategy for the genetic manipulation of English elm. In: Ahuja MR et al. (eds) Somatic cell genetics and molecular genetics of trees. Kluwer, Dordrecht, pp 105–112
Fenning TM, Tymens SS, Gartland JS, Brasier CM, Gartland KMA (1996b) Transformation and regeneration of English Elm using wild-type Agrobacterium tumefaciens. Plant Sci 116:37–46
Fink CVM, Sticklen MB, Lineberger RD, Domir SC (1986) In vitro organogenesis from shoot tip, internode, and leaf explants of Ulmus × “Pioneer”. Plant Cell, Tissue Organ Cult 7:237–245
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell-cycle in intact plant-tissues. Science 220: 1049–1051
Gartland JS, McHugh AT, Brasier CM, Irvine RJ, Fenning TM, Gartland KMA (2000) Regeneration of phenotypically normal English Elm (Ulmus procera) plantlets following transformation with an Agrobacterium tumefaciens binary vector. Tree Physiol 20:901–907
Gartland JS, Brasier CM, Fenning TM, Birch R, Gartland KMA (2001) Ri-plasmid mediated transformation and regeneration of Ulmus procera (English Elm). Plant Growth Regul 33:123–129
George MW, Tripepi RR (1994) Cytokinins, donor plants and time in culture affect shoot regenerative capacity of American Elm leaves. Plant Cell Tissue Organ Cult 39:27–36
Kapaun JA, Cheng ZM (1997) Plant regeneration from leaf tissues of Siberian Elm. HortScience 32:301–303
Karnosky DF, Michler RA, Lange DD (1982) Hormonal control of shoot and root induction in hypocotyl callus cultures of American Elm. In Vitro 18:275
Merkle SA, Parrot WA, Flinn BS (1995) Morphogenic aspects of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer, Dordrecht, pp 155–203
Misra S (1995) Molecular analysis of zygotic and conifer somatic embryos. In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants, vol 1. Kluwer, Dordrecht, pp 119–142
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Pinto G, Valentim H, Costa A, Castro S, Santos C (2002) Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree. In Vitro Cell Dev Biol-Plant: 6:569–572
Raemakers CJJM, Jacobson E, Visser RGF (1995) Secondary somatic embryogenesis and application in plant breeding. Euphytica 81:93–107
Richens RH (1983) Elm. Cambridge University Press, Cambridge
Sousa A, Conde P, Santos C, Correia M, Costa A, Caldeira G (1998) Micropropagation and regeneration of Ulmus minor Mill. In: BIOTEC 98: 4th Iberian Congr Biotechnol. Ibero-American Meeting on Biotechnology. Guimarães, Portugal
Sticklen MB, Sherald JL (1993) Dutch elm disease research. Cellular and molecular approaches. Springer, Berlin Heidelberg New York
Sticklen MB, Domir SC, Lineberger RD (1986) Shoot regeneration from protoplasts of Ulmus × “Pioneer”. Plant Sci 47:29–34
Stipes RJ, Campana RJ (1981) Compendium of elm diseases. The American Phytopathological Society, St Paul
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by D. Bartels
Rights and permissions
About this article
Cite this article
Conde, P., Loureiro, J. & Santos, C. Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill.. Plant Cell Rep 22, 632–639 (2004). https://doi.org/10.1007/s00299-003-0735-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-003-0735-1