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Arabitol dehydrogenase as a selectable marker for rice

  • Genetic Transformation and Hybridization
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Abstract

Arabitol dehydrogenase has been adapted for use as a plant selectable marker. Arabitol is a five-carbon sugar alcohol that can be used by E. coli strain C, but not by the laboratory K12 strains. The enzyme converts the non-plant-metabolizable sugar arabitol into xylulose, which is metabolized by plant cells. Rice was transformed with a plant-expression-optimized synthetic gene using Biolistic-mediated transformation. Selection on 2.75% arabitol and 0.25% sucrose yielded a transformation efficiency (9.3%) equal to that obtained with hygromycin (9.2%). Molecular analyses showed that the atlD gene was integrated into the rice genome of selected plants and was inherited in a Mendelian manner. This study indicates that arabitol could serve as an effective means of plant selection.

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Acknowledgements

This work was funded by NSF grant 9975827 and by federal and state monies allocated to the Georgia Agricultural Experiment Stations. The assistance of Lauren Stanchek, Ukeme Ukoh and Cheryl Smith with the plant cultures is greatly appreciated, as is the assistance of Tara Davis who evaluated the digestibility of ATLD

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Author notes

  1. P. M. Kane

    Present address: Pioneer Hi-Bred International, 7100 NW 62nd Avenue, Johnston, IA, 50131-1014, USA

Authors and Affiliations

  1. Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Road, Athens, GA, 30602-6810, USA

    P. R. LaFayette, P. M. Kane, B. H. Phan & W. A. Parrott

Authors
  1. P. R. LaFayette
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  2. P. M. Kane
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  3. B. H. Phan
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  4. W. A. Parrott
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Correspondence to W. A. Parrott.

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Communicated by R.J. Rose

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LaFayette, P.R., Kane, P.M., Phan, B.H. et al. Arabitol dehydrogenase as a selectable marker for rice. Plant Cell Rep 24, 596–602 (2005). https://doi.org/10.1007/s00299-005-0015-3

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  • DOI: https://doi.org/10.1007/s00299-005-0015-3

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