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Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion

  • Genetic Transformation and Hybridization
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Abstract

Helianthus maximiliani is one of the wild Helianthus species with the genes for resistance to many pathogens including Sclerotinia sclerotiorum. Unfortunately, a transfer of disease resistance genes from this species into the cultivated sunflower is limited by its poor crossability with the cultivated sunflower and sterility of interspecific hybrids. To overcome this problem, mesophyll protoplasts of Sclerotinia sclerotiorum-resistant clone of H. maximiliani were electrically fused with etiolated hypocotyl protoplasts of the cultivated sunflower inbred line PH-BC1-91A. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. Developed microcalluses were released from the agarose and transferred into solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mg l−1 BAP and 0.01 mg l−1 NAA after the treatment with a high concentration of 2,4 D for a limited period of time. A morphological and RAPD analysis confirmed a hybrid nature of the regenerated plants.

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Abbreviations

2,4 D:

2,4-Dichlorophenoxyacetic acid

ACO:

Aconitase

ACP:

Acid Phosphatase

BAP:

6-Benzylaminopurine

IAA:

3-Indolylacetic acid

IBA:

4-(3-Indolyl)butanoic acid

MDH:

Malate Dehydrogenase

NAA:

1-Naphthylacetic acid

PCR:

Polymerase Chain Reaction

PEG:

Polyethylene Glycol

PGD:

6-Phosphogluconate Dehydrogenase

PGM:

Phosphogluco Mutase

PHI:

Phosphohexose Isomerase

RAPD:

Random Amplified Polymorphic DNA

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Acknowledgements

This work was supported by the Serbian Ministry of Science, Technology and Development as a part of the project BTR.5.02.0401.B.

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Authors and Affiliations

  1. National Laboratory for Seed Testing, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia

    Ksenija Taski-Ajdukovic

  2. Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia

    Dragana Vasic & Nevena Nagl

Authors
  1. Ksenija Taski-Ajdukovic
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  2. Dragana Vasic
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  3. Nevena Nagl
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Correspondence to Ksenija Taski-Ajdukovic.

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Communicated by A. Atanassov

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Taski-Ajdukovic, K., Vasic, D. & Nagl, N. Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion. Plant Cell Rep 25, 698–704 (2006). https://doi.org/10.1007/s00299-006-0134-5

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  • DOI: https://doi.org/10.1007/s00299-006-0134-5

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