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Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis–banana interaction

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Abstract

Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA library was constructed to identify transcripts induced at late stages of infection in the host and the pathogen. In addition, a full-length cDNA library was created from the same mRNA starting material as the SSH library. The SSH procedure was effective in identifying specific genes predicted to be involved in plant–fungal interactions and new information was obtained mainly about genes and pathways activated in the plant. Several plant genes predicted to be involved in the synthesis of phenylpropanoids and detoxification compounds were identified, as well as pathogenesis-related proteins that could be involved in the plant response against M. fijiensis infection. At late stages of infection, jasmonic acid and ethylene signaling transduction pathways appear to be active, which corresponds with the necrotrophic life style of M. fijiensis. Quantitative PCR experiments revealed that antifungal genes encoding PR proteins and GDSL-like lipase are only transiently induced 30 days post inoculation (dpi), indicating that the fungus is probably actively repressing plant defense. The only fungal gene found was induced 37 dpi and encodes UDP-glucose pyrophosphorylase, an enzyme involved in the biosynthesis of trehalose. Trehalose biosynthesis was probably induced in response to prior activation of plant antifungal genes and may act as an osmoprotectant against membrane damage.

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Abbreviations

BLSD:

Black leaf streak disease

CTAB:

Cetyl trimethyl ammonium bromide

Dpi:

Days post inoculation

EST:

Expressed sequence tag

ET:

Ethylene

FA:

Fatty acid

HCN:

Hydrogen cyanide

Hpi:

Hours post inoculation

INIBAP:

International network for the improvement of banana and plantain

ITS:

Internal transcribed spacer

JA:

Jasmonate

JA-Ile:

Jasmonyl-isoleucine

NCBI:

National Center for Biotechnology Information

NR:

Non-redundant

PCR:

Polymerase chain reaction

PDB:

Potato dextrose broth

PR:

Pathogenesis related

qPCR:

Quantitative polymerase chain reaction

RT:

Reverse transcriptase

SA:

Salicylic acid

SSH:

Suppression subtractive hybridization

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Acknowledgments

The authors thank Yelenys Alvarado-Capó (Instituto de Biotecnología de las Plantas, Cuba) for fruitful discussions concerning the design of the cDNA libraries construction. We are grateful to Luis Perez Vicente (INISAV, Cuba) and Joke Pannecoucque (Ghent University, Belgium) for critical comments on the manuscript. This research was performed in the frame of the Institutional University Cooperation Programme with Universidad Central “Marta Abreu” de Las Villas, supported by the Flemish Interuniversity Council (IUC UCLV/VLIR). This work also was supported in part by INIBAP (currently Bioversity International) through the project ‘Molecular analysis of Mycosphaerella fijiensis–banana interactions’ (LOA 2003-04). David De Vleesschauwer is indebted to the FWO-Vlaanderen for a postdoctoral fellowship.

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Authors and Affiliations

  1. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní km 5.5, 54 830, Santa Clara, Cuba

    Orelvis Portal, Yovanny Izquierdo, Aminael Sánchez-Rodríguez, Milady Mendoza-Rodríguez, Mayra Acosta-Suárez, Bárbara Ocaña & Elio Jiménez

  2. Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Orelvis Portal, Yovanny Izquierdo, David De Vleesschauwer & Monica Höfte

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  1. Orelvis Portal
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  2. Yovanny Izquierdo
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  3. David De Vleesschauwer
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  4. Aminael Sánchez-Rodríguez
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  9. Monica Höfte
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Correspondence to Monica Höfte.

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Communicated by E. Guiderdoni.

O. Portal and Y. Izquierdo contributed equally to the article.

A contribution to the Special Issue: Plant Biotechnology in Support of the Millennium Development Goals.

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Portal, O., Izquierdo, Y., De Vleesschauwer, D. et al. Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis–banana interaction. Plant Cell Rep 30, 913–928 (2011). https://doi.org/10.1007/s00299-011-1008-z

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  • DOI: https://doi.org/10.1007/s00299-011-1008-z

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