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Overexpression of Arabidopsis MYB96 confers drought resistance in Camelina sativa via cuticular wax accumulation

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Abstract

Key message

Camelina has been highlighted as an emerging oilseed crop. Transgenic Camelina plants overexpressing Arabidopsis MYB96 exhibited drought resistance by activating expression of Camelina wax biosynthetic genes and accumulating wax load.

Abstract

Camelina (Camelina sativa L.) is an oilseed crop in the Brassicaeae family with potential to expand biofuel production to marginal land. The aerial portion of all land plants is covered with cuticular wax to protect them from desiccation. In this study, the Arabidopsis MYB96 gene was overexpressed in Camelina under the control of the CaMV35S promoter. Transgenic Camelina plants overexpressing Arabidopsis MYB96 exhibited normal growth and development and enhanced tolerance to drought. Deposition of epicuticular wax crystals and total wax loads increased significantly on the surfaces of transgenic leaves compared with that of non-transgenic plants. The levels of alkanes and primary alcohols prominently increased in transgenic Camelina plants relative to non-transgenic plants. Cuticular transpiration occurred more slowly in transgenic leaves than that in non-transgenic plants. Genome-wide identification of Camelina wax biosynthetic genes enabled us to determine that the expression levels of CsKCS2, CsKCS6, CsKCR1-1, CsKCR1-2, CsECR, and CsMAH1 were approximately two to sevenfold higher in transgenic Camelina leaves than those in non-transgenic leaves. These results indicate that MYB96-mediated transcriptional regulation of wax biosynthetic genes is an approach applicable to generating drought-resistant transgenic crops. Transgenic Camelina plants with enhanced drought tolerance could be cultivated on marginal land to produce renewable biofuels and biomaterials.

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Abbreviations

VLCFAs:

Very long-chain fatty acids

KCS:

β-Ketoacyl-CoA synthase

KCR:

β-Ketoacyl-CoA reductase

HCD:

β-Hydroxyacyl-CoA dehydratase

ECR:

Enoyl-CoA reductase

CER:

Eceriferum

MAH1:

Mid-chain alkane hydroxylase1

FAR3/CER4:

Fatty acyl-CoA reductase

WSD1:

Bifunctional wax synthase/acyl-CoA:diacylglycerol acyltransferase

GL1:

Glossy1

WSL1:

Wax crystal sparse leaf1

WDA1:

Wax-deficient anther1

DWA1:

Drought-induced wax accumulation

LACS:

Long-chain acyl-CoA synthetase

TF:

Transcription factors

SEM:

Scanning electron microscopy

GC:

Gas chromatography

RT-PCR:

Reverse transcription-polymerase chain reaction

RB:

Right border

E9-T:

Pea rbcs-E9 terminator

BAR:

Bialaphos-resistance gene

NOS-P:

Nopaline synthase gene promoter

35S-P:

Cauliflower mosaic virus 35S promoter

NOS-T:

Nopaline synthase gene terminator

LB:

Left border

NT:

Non-transgenic plants

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Acknowledgments

We thank Jin Hee Jung (HistoGenetics LLC) for technical assistance. This work was supported by grants from the Next-Generation BioGreen 21 Program (No. PJ008203) of the Rural Development Administration, Republic of Korea, and the National Research Foundation (2013R1A2A2A01015672) of Korea.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Bioenergy Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Korea

    Saet Buyl Lee, Hyojin Kim, Ryeo Jin Kim & Mi Chung Suh

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  1. Saet Buyl Lee
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  2. Hyojin Kim
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Correspondence to Mi Chung Suh.

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Communicated by Jeong Sheop Shin.

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Lee, S.B., Kim, H., Kim, R.J. et al. Overexpression of Arabidopsis MYB96 confers drought resistance in Camelina sativa via cuticular wax accumulation. Plant Cell Rep 33, 1535–1546 (2014). https://doi.org/10.1007/s00299-014-1636-1

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