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Thidiazuron-induced abnormalities in plant tissue cultures

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Abstract

Thidiazuron (TDZ) is a proven effective and potent synthetic plant growth regulator for organogenic, regeneration, and developmental pathways, including axillary and adventitious shoot proliferation, somatic embryogenesis, and in vitro flowering. TDZ has facilitated the establishment of in vitro cultures for several plant species, especially woody and recalcitrant plants, which has enabled their genetic transformation and improvement. Despite the effectiveness and advantages of using TDZ, several drawbacks are associated with its application in plant tissue culture. This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.

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References

  • Ahmad N, Faisal M (2018) Thidiazuron: From urea derivative to plant growth regulator. Springer, Singapore, p 489

    Google Scholar 

  • Ahmad Z, Shahzad A (2018) Thidiazuron influenced morphogenesis in some medicinal plants. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 231–246

    Google Scholar 

  • Ahmad N, Srivastava R, Anis M (2006) Improvement in carnation shoot multiplication using thidiazuron in vitro. Propag Ornam Plants 6:109–113

    Google Scholar 

  • Ahmed MR, Anis M (2012) Role of TDZ in the quick regeneration of multiple shoots from nodal explant of Vitex trifolia L.—an important medicinal plant. Appl Biochem Biotechnol 168:957–966

    CAS  PubMed  Google Scholar 

  • Ahmed MR, Anis M (2014) Changes in activity of antioxidant enzymes and photosynthetic machinery during acclimatization of micropropagated Cassia alata L. plantlets. In Vitro Cell Dev Biol Plant 50:601–609

    CAS  Google Scholar 

  • Akasaka Y, Daimon H, Mii M (2000) Improved plant regeneration from cultured leaf segments in peanut (Arachis hypogaea L.) by limited exposure to thidiazuron. Plant Sci 156:169–175

    CAS  PubMed  Google Scholar 

  • Anderson WC (1975) Propagation of rhododendrons by tissue culture. Part 1. Development of a culture medium for multiplication of shoots. Proc Intl Plant Prop Soc 25:129–134

    Google Scholar 

  • Arndt FR, Rusch R, Stillfried HV, Hanisch B, Martin WC (1976) SN 49537, a new defoliant [abstract]. Plant Physiol 57:99

    Google Scholar 

  • Azria D, Bhalla PL (2000) Plant regeneration from mature embryo-derived callus of Australian rice (Oryza sativa L.) varieties. Aust J Agric Res 51:305–312

    Google Scholar 

  • Bairu MW, Stirk WA, Staden JV (2009) Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tiss Org Cult 98:239–248

    Google Scholar 

  • Baskaran P, Moyo M, Van Staden J (2014) In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum. S Afr J Bot 90:74–79

    CAS  Google Scholar 

  • Bhagwat B, Vieira LGE, Erickson LR (1996) Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberellic acid. Plant Cell Tiss Org Cult 46:1–7

    CAS  Google Scholar 

  • Bhattacharyya P, Kumaria S, Diengdoh R, Tandon P (2014) Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid. Meta Gene 2:489–504

    PubMed  PubMed Central  Google Scholar 

  • Bohmer P, Meyer B, Jacobson HJ (1995) Thidiazuron induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Rep 15:26–29

    CAS  PubMed  Google Scholar 

  • Bosela MJ, Michler CH (2008) Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types. In Vitro Cell Dev Biol Plant 44:316–329

    CAS  Google Scholar 

  • Cao X, Hammerschlag FA (2000) Improved shoot organogenesis from leaf explants of highbush blueberry. HortScience 35:945–947

    CAS  Google Scholar 

  • Chakrabarty D, Park SY, Ali MB, Shin KS, Paek KY (2006) Hyperhydricity in apple: ultrastructural and physiological aspects. Tree Physiol 26:377–388

    CAS  PubMed  Google Scholar 

  • Chen JT, Chang WC (2000) Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium sweet sugar. Plant Cell Tiss Org Cult 62:95–100

    CAS  Google Scholar 

  • Chen J, Wu L, Hu B, Yi X, Liu R, Deng Z, Xiong X (2014) The influence of plant growth regulators and light quality on somatic embryogenesis in china rose (Rosa chinensis Jacq.). J Plant Growth Regul 33:295–304

    CAS  Google Scholar 

  • Chengalrayan K, Gallo-Meagher M (2001) Effect of various growth regulators on shoot regeneration of sugarcane. In Vitro Cell Dev Biol Plant 37:434–439

    CAS  Google Scholar 

  • Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Org Cult 106:279–288

    CAS  Google Scholar 

  • Debnath SC (2005) Micropropagation of lingonberry: influence of genotype, explant, orientation and overcoming TDZ-induced inhibition of shoot elongation using zeatin. HortScience 40:185–188

    CAS  Google Scholar 

  • Debnath SC (2009) A two-step procedure for adventitious shoot regeneration on excised leaves of lowbush blueberry. In Vitro Cell Dev Biol Plant 45:122–128

    Google Scholar 

  • Debnath SC (2018) Thidiazuron in micropropagation of small fruits. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 139–158

    Google Scholar 

  • Deepa AV, Anju M, Thomas TD (2018) The applications of TDZ in medicinal plant tissue culture. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 297–316

    Google Scholar 

  • Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2006a) A simple method for mass propagation of Spathiphyllum cannifolium using an airlift bioreactor. In Vitro Cell Dev Biol Plant 42:291–297

    CAS  Google Scholar 

  • Dewir YH, Chakrabarty D, Ali MB, Hahn EJ, Paek KY (2006b) Lipid peroxidation and antioxidant enzyme activities of Euphorbia millii hyperhydric shoots. Environ Exp Bot 58:93–99

    CAS  Google Scholar 

  • Dewir YH, Indoliya Y, Chakrabarty D, Paek KY (2014) Biochemical and physiological aspects of hyperhydricity in liquid culture system. In: Paek KY, Murthy HN, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Springer, Berlin, pp 693–703

    Google Scholar 

  • Dewir YH, El-Mahrouk ME, El-Banna AN (2015) In vitro propagation and preliminary results of Agrobacterium-mediated genetic transformation of Cordyline fruticosa. S Afr J Bot 98:45–51

    CAS  Google Scholar 

  • Dewir YH, Murthy HN, Ammar MH, Alghamdi SS, Al-Suhaibani NA, Alsadon AA, Paek KY (2016) In vitro rooting of leguminous plants: difficulties, alternatives, and strategies for improvement. Hortic Environ Biotechnol 57:311–322

    CAS  Google Scholar 

  • Dewir YH, Aldubai AA, El-Hendawy S, Alsadon AA, Seliem MK, Naidoo Y (2018) Micropropagation of buttonwood tree (Conocarpus erectus) through axillary shoot proliferation. HortScience 53:687–691

    Google Scholar 

  • Driver JA, Kuniyuki AH (1984) In vitro propagation of Paradox walnut rootstock. HortScience 19:507–509

    Google Scholar 

  • Ellis DD, Barczynska H, McCown BH, Nelson N (1991) Comparison of BA, zeatin, and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants. Plant Cell Tiss Org Cult 27:281–287

    CAS  Google Scholar 

  • El-Mahrouk ME, Dewir YH, Omar AMK (2010) In vitro propagation of adult strawberry tree (Arbutus unedo L.) through adventitious shoots and somatic embryogenesis. Propag Ornam Plants 10:93–98

    Google Scholar 

  • El-Mahrouk ME, Dewir YH, Naidoo Y (2016) Micropropagation and genetic fidelity of the regenerants of Aglaonema ‘valentine’ using randomly amplified polymorphic DNA. HortScience 51:398–402

    CAS  Google Scholar 

  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss Org Cult 80:187–190

    CAS  Google Scholar 

  • Figueiredo SFL, Albarello N, Viana VRC (2001) Micropropagation of Rollinia mucosa (Jacq.) Baill. In Vitro Cell Dev Biol Plant 37:471–475

    CAS  Google Scholar 

  • Franklin G, Sheeba CJ, Sita GL (2004) Regeneration of eggplant (Solanum melongena L.) from root explants. In Vitro Cell Dev Biol Plant 40:188–191

    Google Scholar 

  • Genkov T, Tsoneva P, Ivanova I (1997) Effect of cytokinins on photosynthetic pigments and chlorophyllase activity in in vitro cultures of axillary buds of Dianthus caryophyllus L. J Plant Growth Regul 16:169–172

    CAS  Google Scholar 

  • Goffreda JC, Scopel AL, Fiola JA (1995) Indole butyric acid induces regeneration of phenotypically normal apricot (Prunus armeniaca L.) plants from immature embryos. Plant Growth Regul 17:41–46

    CAS  Google Scholar 

  • Gostimsky SA, Kokaeva ZG, Konovalov FA (2005) Studying plant genome variation using molecular markers. Russ J Genet 41:378–388

    CAS  PubMed  PubMed Central  Google Scholar 

  • Graner EM, Oberschelp GP, Brondani GE, Batagin-Piotto KD, de Almeida CV, de Almeida M (2013) TDZ pulsing evaluation on the in vitro morphogenesis of peach palm. Physiol Mol Biol Plants 19:283–288

    PubMed  PubMed Central  Google Scholar 

  • Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10:8984–9000

    CAS  Google Scholar 

  • Han BH, Park BM (2008) In vitro micropropagation of Philodendron cannifolium. J Plant Biotechnol 35:203–208

    Google Scholar 

  • Han BH, Yae BW, Yu HJ, Peak KY (2005) Improvement of in vitro micropropagation of Lilium oriental hybrid ‘Casablanca’ by the formation of shoots with abnormally swollen basal plates. Sci Hortic 103:351–359

    CAS  Google Scholar 

  • Harda T, Ishikawa R, Niizeki M, Saito K (1992) Pollen derived rice calli that have large deletions in plastid DNA do not require protein synthesis in plastids for growth. Mol Gen Genet 233:145–150

    Google Scholar 

  • Hare PD, Staden J, Van Staden J (1994) Inhibitory effect of TDZ on the activity of cytokinin oxidase isolated form soybean callus. Plant Cell Physiol 35:11221–11125

    Google Scholar 

  • Horgan R, Burch LR, Palni LMS (1988) Cytokinin oxidase and the degradative metabolism of cytokinins, plant growth substances. In: Pharis RP, Rood SB (eds) Plant growth substances. Springer, Berlin, pp 282–290

    Google Scholar 

  • Huang L-C, Huang B-L, Murashige T (1998) A micropropagation protocol for Cinnamomum camphora. In Vitro Cell Dev Biol Plant 34:141–146

    Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119

    CAS  Google Scholar 

  • Hutchinson MJ, Saxena PK (1996) Acetylsalicyclic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium × hortorum Bailey) tissue cultures. Plant Cell Rep 15:512–515

    CAS  PubMed  Google Scholar 

  • Hutchinson MJ, KrishnaRaj S, Saxena PK (1997a) Inhibitory effect of GA3 on the development of thidiazuron-induced somatic embryogenesis in geranium (Pelargonium × hortorum Bailey) hypocotyl cultures. Plant Cell Rep 16:435–438

    CAS  PubMed  Google Scholar 

  • Hutchinson MJ, Murr DP, KrishnaRaj S, Saxena PK (1997b) Does ethylene play a role in thidiazuron-regulated somatic embryogenesis of geranium (Pelargonium × hortorum) hypocotyl cultures. In Vitro Cell Dev Biol Plant 33:136–141

    CAS  Google Scholar 

  • Ivanova M, Van Staden J (2008) Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla. Plant Cell Tiss Org Cult 92:227–231

    CAS  Google Scholar 

  • Ivanova M, Van Staden J (2011) Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla. Plant Cell Tiss Org Cult 104:13–21

    CAS  Google Scholar 

  • Javed SB, Anis M, Khan PR, Aref IM (2013) In vitro regeneration and multiplication for mass propagation of Acacia ehrenbergiana Hayne: a potential reclaiment of denude arid lands. Agrofor Syst 87:621–629

    Google Scholar 

  • Joersbo M, Brunstedt J, Marcussen J, Okkels FT (1999) Transformation of the endospermous legume guar (Cyamopsis tetragonoloba L.) and analysis of transgene transmission. Mol Breed 5:521–529

    CAS  Google Scholar 

  • Jones M, Yi Z, Murch SJ, Saxena PK (2007) Thidiazuron-induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep 26:13–19

    CAS  PubMed  Google Scholar 

  • Joshi M, Sujatha K, Hazra S (2008) Effect of TDZ and 2, 4-d on peanut somatic embryogenesis and in vitro bud development. Plant Cell Tiss Org Cult 94:85–90

    CAS  Google Scholar 

  • Kacar YA, Byrne PF, Teixeira da Silva JA (2006) Molecular markers in plant tissue culture. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol II, 1st edn. Global Science Books, Ltd., Isleworth, pp 444–449

    Google Scholar 

  • Kadota M, Niimi Y (2003) Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tiss Org Cult 72:261–265

    CAS  Google Scholar 

  • Karavaiko NN, Selivankina SY, Kudryakova NV, Maslova GG, Burkhanova EA, Zubkova NK, Kulaeva ON (2004) Is a 67-kD cytokinin-binding protein from barley and Arabidopsis thaliana leaves involved in the leaf responses to phenylurea derivatives? (a review). Russ J Plant Physiol 51:790–797

    CAS  Google Scholar 

  • Khan MI, Anis M (2012) Modulation of in vitro morphogenesis in nodal segments of Salix tetrasperma Roxb. through the use of TDZ, different media types and culture regimes. Agrofor Sys 86:95–103

    Google Scholar 

  • Kher MM, Joshi D, Nekkala S, Nataraj M, Raykundaliya DP (2014) Micropropagation of Pluchea lanceolata (Oliver & Hiern.) using nodal explant. J Hort Res 22:35–39

    Google Scholar 

  • Khoddamzadeh AA, Sinniah UR, Kadir MA, Kadzimin SB, Mahmood M, Sreeramanan S (2010) Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb. f.) Christenson. Afr J Biotechnol 9:6632–6639

    CAS  Google Scholar 

  • Kim OT, Bang KH, In DS, Kim TS, Seong NS, Cha SW, Ahn JC, Hwang B (2006) Micropropagation of Hypericum erectum Thunberg by using thidiazuron. Korean J Med Crop Sci 14:278–281

    Google Scholar 

  • Kirakosyan A, Gibson D, Kaufman P (2008) The production of dianthrones and phloroglucinol derivatives in St. John’s Wort. In: Ramawat K, Merillon J (eds) Bioactive molecules and medicinal plants. Springer, Berlin

    Google Scholar 

  • Kumari M, Clarke HJ, Small I, Siddique KHM (2009) Albinism in plants: a major bottleneck in wide hybridization, androgenesis and doubled haploid culture. Crit Rev Plant Sci 28:393–409

    CAS  Google Scholar 

  • Kumari P, Singh S, Yadav S, Tran LSP (2018) Pretreatment of seeds with thidiazuron delimits its negative effects on explants and promotes regeneration in chickpea (Cicer arietinum L.). Plant Cell Tiss Org Cult 133:103–114

    CAS  Google Scholar 

  • Lakshmanan P, Taji A (2000) Somatic embryogenesis in leguminous plants. Plant Biol 2:136–148

    CAS  Google Scholar 

  • Lakshmi SG, Raghava SBV (1993) Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci 88:107–112

    Google Scholar 

  • Lata H, Bedir E, Hosick A, Ganzera M, Khan I, Moraes RM (2002) In vitro plant regeneration from leaf-derived callus of Cimicifuga racemosa. Planta Med 68:912–915

    CAS  PubMed  Google Scholar 

  • Li W, Ding C-H, Hu Z, Lu W, Guo G-Q (2003) Relationship between tissue culture and agronomic traits of spring wheat. Plant Sci 164:1079–1985

    CAS  Google Scholar 

  • Lin CS, Chang WC (1998) Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets. Plant Cell Rep 17:617–620

    CAS  PubMed  Google Scholar 

  • Lin SA, Liang CJ, Hsaio HW, Lin MJ, Chang WC (2007) In vitro flowering of green and albino Dendrocalamus latiflorus. New For 34:177–186

    Google Scholar 

  • Liu XN, Zhang XQ, Sun JS (2007) Effects of cytokinins and elicitors on the production of hypericins and hyperforin metabolites in Hypericum sampsonii and Hypericum perforatum. Plant Growth Regul 53:207–214

    CAS  Google Scholar 

  • Liu C, Callow P, Rowland LJ, Hancock JF, Song G-Q (2010) Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars. Plant Cell Tiss Org Cult 103:137–144

    Google Scholar 

  • Lu C-Y (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol Plant 29:92–96

    Google Scholar 

  • Lu C, Nugent G, Wardley-Richardson T, Stephen F, Chandler SF, Young R, Dalling MJ (1991) Agrobacterium-mediated transformation of carnation (Dianthus caryophyllus L.). Nat Biotechnol 9:864–868

    CAS  Google Scholar 

  • Ma G-H, Lü J-F, Teixeira da Silva JA, Zhang X-H, Zhao J-T (2011) Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour). Plant Cell Tiss Org Cult 104:157–162

    CAS  Google Scholar 

  • Mackay WA, Tipton JL, Thompson GA (1995) Micropropagation of Mexican redbud, Cercis canadensis var. mexicana. Plant Cell Tiss Org Cult 43:295–299

    Google Scholar 

  • Magioli C, Rocha APM, De Oliveira DE, Mansur E (1998) Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep 17:661–663

    CAS  PubMed  Google Scholar 

  • Malik KA, Saxena PK (1992)) Regeneration in Phaseolus vulgaris L.: high frequency induction of direct shoot formation in intact seedlings by N6-benzyladenine and thidiazuron. Planta 186:384–389

    CAS  PubMed  Google Scholar 

  • Mante S, Scorza R, Cordts J (1989) A simple, rapid protocol for adventitious shoot development from mature cotyledons of Glycine max cv Bragg. In Vitro Cell Dev Biol 25:385–388

    CAS  Google Scholar 

  • Marcotrigiano M, McGlew SP, Hackett G, Chawla B (1996) Shoot regeneration from tissue- cultured leaves of the American cranberry (Vaccinium macrocarpon). Plant Cell Tiss Org Cult 44:195–199

    Google Scholar 

  • Marks TR, Simpson SE (1994) Factors affecting shoot development in apically dominant Acer cultivars in vitro. J Hortic Sci 69:543–551

    Google Scholar 

  • McCown BH, Lloyd G (1981) Woody plant medium (WPM)—a revised mineral formulation for micro-culture of woody plant species. HortScience 16:453

    Google Scholar 

  • Mehta UJ, Barreto SM, Hazra S (2004) Effect of thidiazuron in germinating tamarind seedlings. In Vitro Cell Dev Biol Plant 40:279–283

    CAS  Google Scholar 

  • Mithila J, Hall JC, Victor JMR, Saxena PK (2003) Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.). Plant Cell Rep 21:408–414

    CAS  PubMed  Google Scholar 

  • Mok MC, Mok DWS (1985) The metabolism of [14C]-thidiazuron in callus tissues of Phaseolus lunatus. Physiol Plant 65:427–432

    CAS  Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ (1982) Cytokinin activity of N-phenyl-N-1, 2, 3-thidiiazol-5ylurea (TDZ). Phytochemistry 21:1509–1511

    CAS  Google Scholar 

  • Murch S, Saxena PK (2001) Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium × hortorum Bailey. Plant Growth Regul 35:269–275

    CAS  Google Scholar 

  • Murch SJ, Victor JMR, Krishnaraj S, Saxena PK (1999) The role of proline in thidiazuron-induced somatic embryogenesis of peanut. In Vitro Cell Dev Biol Plant 35:102–105

    CAS  Google Scholar 

  • Murthy BNS (1997) Morpho-physiological role of thidiazuron in plants. PhD thesis, University of Guelph, Canada

  • Murthy B, Murch S, Saxena PK (1995) Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol Plant 94:268–276

    CAS  Google Scholar 

  • Murthy BNS, Victor J, Singh RPS, Fletcher RA, Saxena PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regul 19:233–240

    CAS  Google Scholar 

  • Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275

    CAS  Google Scholar 

  • Nisler J, Kopečný D, Končitíková R, Zatloukal M, Bazgier V, Berka K, Zalabák D, Briozzo P, Strnad M, Spíchal L (2016) Novel thidiazuron-derived inhibitors of cytokinin oxidase/dehydrogenase. Plant Mol Biol 92:235–248

    CAS  PubMed  Google Scholar 

  • Nobre J (1996) In vitro cloning and micropropagation of Lavandula stoechas from field-grown plants. Plant Cell Tiss Org Cult 46:151–155

    CAS  Google Scholar 

  • Nugent G, Wardley-Richardson T, Lu C (1991) Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.). Plant Cell Rep 10:477–480

    CAS  PubMed  Google Scholar 

  • Oliveira LM, Paiva R, Santanaa JRF, Alves E, Nogueira RC, Pereira FD (2008) Effect of cytokinins on in vitro development of autotrophism and acclimatization of Annona glabra L. In Vitro Cell Dev Biol Plant 44:128–135

    Google Scholar 

  • Pal A, Negi VS, Borthakur D (2012) Efficient in vitro regeneration of Leucaena leucocephala using immature zygotic embryos as explants. Agrofor Syst 84:131–140

    Google Scholar 

  • Papafotiou M, Bertsouklis KF, Trigka M (2013) Micropropagation of Arbutus unedo, A. andrachne, and their natural hybrid, A. × andrachnoides from seedling explants. J Hortic Sci Biotechnol 88:768–775

    CAS  Google Scholar 

  • Park SW, Jeon JH, Kim HS, Park YM, Aswath C, Joung H (2004) Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro. Sci Hortic 99:199–205

    Google Scholar 

  • Perveen S, Anis M (2015) Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree. Agrofor Syst 89:721–733

    Google Scholar 

  • Podwyszyńska M, Niedoba K, Korbin M, Marasek A (2006) Somaclonal variation in micropropagated tulips determined by phenotype and DNA markers. Acta Hortic 714:211–220

    Google Scholar 

  • Podwyszyńska M (2005) Somaclonal variation in micropropagated tulips based on phenotype observation. J Fruit Ornam Plant Res 13:109–122

    Google Scholar 

  • Preece JE, Hutterman CA, Ashby WC, Roth PL (1991) Micro and cutting propagation of silver mapple. I. Results with adult and juvenile propagules. J Am Soc Hortic Sci 116:142–148

    CAS  Google Scholar 

  • Prince AM, Pascual D, Meruelo D, Liebes L, Mazur Y, Dubovi E, Mandel M, Lavie G (2000) Strategies for evaluation of enveloped virus inactivation in red cell concentrates using hypericin. Photochem Photobiol 71:188–195

    CAS  PubMed  Google Scholar 

  • Qu L, Polashock J, Vorsa N (2000) A highly efficient in vitro cranberry regeneration system using leaf explants. HortScience 35:948–952

    CAS  Google Scholar 

  • Radhakrishnan R, Ramachandran A, Kumari BR (2009) Rooting and shooting: dual function of thidiazuron in in vitro regeneration of soybean (Glycine max L.). Acta Physiol Plant 31:1213–1217

    CAS  Google Scholar 

  • Roy AR, Sajeev S, Pattanayak A, Deka BC (2012) TDZ induced micropropagation in Cymbidium giganteum Wall. Ex Lindl. and assessment of genetic variation in the regenerated plants. Plant Growth Regul 68:435–445

    CAS  Google Scholar 

  • Samarfard S, Kadir MA, Kadzimin SB, Saud HM, Ravanfar SA, Danaee M (2014) In vitro propagation and detection of somaclonal variation in Phalaenopsis gigantea as affected by chitosan and thidiazuron combinations. HortScience 49:82–88

    CAS  Google Scholar 

  • Schulze J (2007) Improvements in cereal tissue culture by thidiazuron: a review. Fruit Veg Cereal Sci Biotech 1:64–79

    Google Scholar 

  • Shaik NM, Arha M, Nookaraju A, Gupta SK, Shrivastava S, Yadav AK, Kulkarni PS, Abhilash OU, Vishwakarma RK, Singh S, Tatkare R, Chinnathambi K, Rawal SK, Khan BM (2009) Improved method of in vitro regeneration in Leucaena leucocephala—a leguminous pulpwood tree species. Physiol Mol Biol Plants 15:311–318

    PubMed  PubMed Central  Google Scholar 

  • Shirani S, Mahdavi F, Maziah M (2009) Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoot-tips. Afr J Biotechnol 8:5755–5761

    CAS  Google Scholar 

  • Siddique I, Bukhari NAW, Perveen K, Siddiqui I (2015) Influence of plant growth regulators on in vitro shoot multiplication and plantlet formation in Cassia angustifolia Vahl. Braz Arch Biol Technol 58:686–691

    CAS  Google Scholar 

  • Silué N, Koné T, Soumahoro AB, Koné M (2016) In vitro shoot tip multiplication of bambara groundnut [Vigna subterranea (L.) Verdc.]. Plant Cell Tiss Org Cult 127:603–611

    Google Scholar 

  • Sivanesan I, Song JY, Hwang SJ, Jeong BR (2011) Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant. Plant Cell Tiss Org Cult 105:55–63

    Google Scholar 

  • Song G-Q, Sink KC (2004) Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.). Plant Cell Rep 23:475–484

    CAS  PubMed  Google Scholar 

  • Teixeira da Silva JA (2003) Chrysanthemum: advances in tissue culture, postharvest technology, genetics and transgenic biotechnology. Biotechnol Adv 21:715–766

    CAS  PubMed  Google Scholar 

  • Teixeira da Silva JA (2012) Callus, calluses or calli: multiple plurals? Asian Australas J Plant Sci Biotechnol 6:125–126

    Google Scholar 

  • Teixeira da Silva JA (2015) Negative results: negative perceptions limit their potential for increasing reproducibility. J Negat Results BioMed 14:12

    PubMed  PubMed Central  Google Scholar 

  • Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tiss Org Cult 66:9–16

    CAS  Google Scholar 

  • Unal BT (2018) Thidiazuron as an elicitor in the production of secondary metabolite. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 463–470

    Google Scholar 

  • Us-Camas R, Castillo-Castro E, Aguilar-Espinosa M, Limones-Briones V, Rivera-Madrid R, Robert-Díaz ML, de la Peña C (2017) Assessment of molecular and epigenetic changes in the albinism of Agave angustifolia Haw. Plant Sci 263:156–167

    CAS  PubMed  Google Scholar 

  • Van Sambeek JW, Lambus LJ, Kahn SB, Preece JE (1997) In vitro establishment of tissues from adult black walnut. In: Van Sambeek JW (ed), Knowledge for the future of black walnut. Proceedings of the fifth black walnut symposium, 28–31 July 1996, USDA Forest Service, North Central Forest Experiment Station, pp 78–92

  • Victorio CP, Arruda RDCDO, Riehl CAS, Lage CLS (2011) Leaf volatiles and secretory cells of Alpinia zerumbet (pers.) Burtt et Smith (Zingiberaceae). Nat Prod Res 25:939–948

    CAS  PubMed  Google Scholar 

  • Vieira de Vasconcelos AG, Tomas LF, Camara TR, Willadino L (2012) Hyperhydricity: a metabolic disorder. Cienc Rural 42:837–844

    Google Scholar 

  • Vinoth A, Ravindhran R (2018) In vitro morphogenesis of woody plants using thidiazuron. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 211–230

    Google Scholar 

  • Wang ZH, Wang L, Ye QS (2009) High frequency early flowering from in vitro seedlings of. Dendrobium nobile. Sci Hortic 122:328–331

    CAS  Google Scholar 

  • White P, Broadley M (2003) Calcium in plants. Ann Bot 92:487–511

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yao O-Y, Chen Y-L, Lü J-F, Teixeira da Silva JA, Zhang X-H, Ma G-H (2016) Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia WT Wang. Sci Rep 6:24662

    Google Scholar 

  • Yew CK, Balakrishnan B, Sundasekaran J, Subramaniam S (2010) The effect of cytokinins on in vitro shoot length and multiplication of Hymenocallis littoralis. J Med Plants Res 4:2641–2646

    CAS  Google Scholar 

  • Yip WK, Yang SF (1986) Effect of thidiazuron, a cytokinin-active urea derivative, in cytokinin-dependent ethylene production system. Plant Physiol 80:515–519

    CAS  PubMed  PubMed Central  Google Scholar 

  • Zeng X, Tang R, Guo H, Ke S, Teng B, Hung YH, Xu Z, Xie XM, Hsieh TF, Zhang XQ (2017) A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter. Plant Mol Biol 94:137–148

    CAS  PubMed  Google Scholar 

  • Zhang CR, Huang XL, Wu JY, Feng BH, Chen YF (2006) Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa). Physiol Plant 128:732–739

    CAS  Google Scholar 

  • Zhang Y-Y, Cheng Q-W, Niu M-Y, Liang H-Z, Yan H-F, Zhang X-H, Teixeira da Silva JA, Ma G-H (2016) Plant regeneration via direct and callus-mediated organogenesis from leaf explants of Chirita swinglei (Merr.) WT Wang. In Vitro Cell Dev Biol Plant 52:521–529

    Google Scholar 

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Acknowledgements

Y.H. Dewir and Y. Naidoo extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP# 0064.

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Authors and Affiliations

  1. Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Yaser Hassan Dewir &  Nurmansyah

  2. Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt

    Yaser Hassan Dewir

  3. School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban, 4000, South Africa

    Yougasphree Naidoo

  4. Ikenobe, Japan

    Jaime A. Teixeira da Silva

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  1. Yaser Hassan Dewir
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  2. Nurmansyah
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  3. Yougasphree Naidoo
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  4. Jaime A. Teixeira da Silva
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Correspondence to Yaser Hassan Dewir, Nurmansyah, Yougasphree Naidoo or Jaime A. Teixeira da Silva.

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Communicated by Neal Stewart.

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Dewir, Y.H., Nurmansyah, Naidoo, Y. et al. Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Rep 37, 1451–1470 (2018). https://doi.org/10.1007/s00299-018-2326-1

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