Abstract
Increasing crop production to meet the demands of a growing population depends largely on crop improvement through new plant-breeding techniques (NPBT) such as genome editing. CRISPR/Cas systems are NPBTs that enable efficient target-specific gene editing in crops, which is supposed to accelerate crop breeding in a way that is different from genetically modified (GM) technology. Herein, we review the applications of CRISPR/Cas systems in crop breeding focusing on crop domestication, heterosis, haploid induction, and synthetic biology, and summarize the screening methods of CRISPR/Cas-induced mutations in crops. We highlight the importance of molecular characterization of CRISPR/Cas-edited crops, and pay special attentions to emerging highly specific genome-editing tools such as base editors and prime editors. We also discuss future improvements of CRISPR/Cas systems for crop improvement.
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Acknowledgements
This work was partially supported by grants from the China National Transgenic Plant Special Fund (2016ZX08012-002, 2017ZX08013-001) and the Programme of Introducing Talents of Discipline to Universities (111 Project, B14016).
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Biswas, S., Zhang, D. & Shi, J. CRISPR/Cas systems: opportunities and challenges for crop breeding. Plant Cell Rep 40, 979–998 (2021). https://doi.org/10.1007/s00299-021-02708-2
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DOI: https://doi.org/10.1007/s00299-021-02708-2