Somatic embryogenesis and plant regeneration from suspension cultures of Acanthopanax koreanum Nakai

Abstract

High-frequency somatic embryogenesis was achieved from an embryogenic cell suspension culture of Acanthopanax koreanum Nakai. Stem segments were cultured on Murashige and Skoog (MS) medium containing auxins and cytokinins. Opaque and friable embryogenic callus formed on MS medium with 4.5 µm 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 µm kinetin or zeatin, but was highest on medium containing 4.5 µm 2,4-D alone. Embryogenic calli were transferred to MS liquid medium containing 4.5 µm 2,4-D and maintained by subculture at 2-week intervals. Initiation of somatic embryogenesis and development up to the globular stage from embryogenic cell clumps occurred in medium containing 0.45 µm 2,4-D, whereas maturation and germination of somatic embryos occurred in MS medium lacking 2,4-D. Cytokinin treatment suppressed the normal growth of embryos, but stimulated secondary somatic embryogenesis from the surfaces of primary embryos. Plants from somatic embryos were acclimatized in a greenhouse.