Skip to main content
SpringerLink
Log in

Synteny mapping in river buffalo

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP-YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology between cattle and river buffalo, comparative mapping predicts the localization of these syntenic groups on river buffalo Chromosomes (Chrs) :BBU7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been previously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adkison, L.R., Leung, D.W., Womack, J.E. (1988). Somatic cell mapping and restriction fragment length analysis of bovine α and β interferon gene families. Cytogenet. Cell Genet. 47, 62–65.

    Article  PubMed  CAS  Google Scholar 

  • Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teal, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel D.J.S. (1994). A genetic map of the bovine genome. Nature Genet. 6, 227–235.

    Article  PubMed  CAS  Google Scholar 

  • Blin, N., Stafford, D.W. (1976). A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 3, 2303–2308.

    PubMed  CAS  Google Scholar 

  • Buckland, R.A., Evans, H.J. (1978). Cytogenic aspects of phylogeny in the Bovidae. 1: G-banding. Cytogenet. Cell Genet. 21, 42–63.

    Article  PubMed  CAS  Google Scholar 

  • Bunch, T.D., Nadler, C.F. (1980). Giemsa-band patterns of the tahr and chromosomal evolution of the tribe caprini. J. Hered. 71, 110–116.

    PubMed  CAS  Google Scholar 

  • Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.

    Article  PubMed  CAS  Google Scholar 

  • Chowdhary, R., Chowdhary, B.P., Johansson, M., Gustavsson, I. (1993). The gene for bovine interferon gamma (IFNG) maps to the q22–q24 bands of chromosome 5 in cattle. Hereditas 119, 11–14.

    Article  PubMed  Google Scholar 

  • De Hondt, H.A., Bosma, A.A., den Bieman, M., de Haan, N.A., van Zut-phen, L.F.M. (1991). Gene mapping in river buffalo (Bubalus bubalis L.). Genet. Sel. Evol., Suppl. 1, 104s-108s.

    Article  Google Scholar 

  • Di Berardino, D., Iannuzzi, L. (1981). Chromosome banding homologies in Swamp and Murrah buffalo. J. Hered. 72, 183–188.

    PubMed  Google Scholar 

  • Di Berardino, D., Iannuzzi, L. (1984). Detailed description of RBA banded chromosomes of river buffalo (Bubalis bubalis L.). Genet. Sel. Evol. 16, 249–260.

    Article  Google Scholar 

  • El Nahas, S.M., de Hondt, A., Othman, O.S., Bosma, A., de Haan, N. (1993). Assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo-hamster hybrid cells. J Anim. Breed. Genet. 110, 182–185.

    Google Scholar 

  • Evans, HJ., Buckland, A., Samner, A.T. (1973). Chromosome homology and heterochromatin in goat, sheep, and ox studies by banding techniques. Chromosoma 42, 383–402.

    Article  PubMed  CAS  Google Scholar 

  • Feinberg, A.P., Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Bio-chem. 132, 6–13.

    CAS  Google Scholar 

  • Feinberg, A.P., Vogelstein, B. (1984). Addendum: a technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 266–267.

    Article  PubMed  CAS  Google Scholar 

  • Ferretti, L., Leone, P., Pilla, F., Zhang, Y., Guerin, G. (1994). Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping. Anim. Genet., in press.

  • Fries, R., Hediger, R., Stranzinger, G. (1986). Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim. Genet. 17, 287–294.

    Article  PubMed  CAS  Google Scholar 

  • Fries, R., Eggen, A., Womack, J.E. (1993). The bovine genome map. Mamm. Genome 4, 405–428.

    Article  PubMed  CAS  Google Scholar 

  • Gallagher, D.S., Womack, J.E. (1992). Chromosome conservation in the Bovidae. J. Hered. 83, 287–298.

    PubMed  Google Scholar 

  • Gallagher, D.S., Threadgill, D.W., Jackson, A.P., Pasham, P., Womack, J.E. (1991). Somatic mapping of bovine syntenic group. Cytogenet. Cell Genet. 56, 154–156.

    Article  PubMed  CAS  Google Scholar 

  • Gallagher, D.S., Ryan, A.M., Liou, L.S., Sastry, K.N., Womack, J.E. (1993). Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescence in situ localization to chromosome 28. Mamm. Genome 4, 716–719.

    Article  PubMed  CAS  Google Scholar 

  • Gu, F., Chowdhary, B.P. Andersson, L., Harbitz, I., Gustavsson, I. (1992). Assignment of the bovine immunoglobulin gamma heavy chain (IGHG) gene to chromosome 21q24 band by in situ hybridization. Hereditas 117, 237–240.

    Article  PubMed  CAS  Google Scholar 

  • Hassanane, M.S., Gu, F., Chowdhary, B.P., Anderson, L., Gustavsson, I. (1993). In situ hybridization mapping of the immunoglobulin gamma heavy chain (IGHG) gene to chromosome 20 1q23–q25 in river buffaloes. Hereditas 118, 285–288.

    Article  PubMed  CAS  Google Scholar 

  • Hassanane, M.S., Chowdhary, B.P., Anderson, L., Gustavsson, I. (1994). Mapping the interferon gamma (IFNG) gene in river buffalo and swamp buffaloes by in situ hybridization. Hereditas 120, 29–33.

    Article  PubMed  CAS  Google Scholar 

  • Hediger, R., Ansari, H.A., Stranzinger, G.F. (1991). Chromosome banding and gene localization support extensive conservation of chromosome structure between cattle and sheep. Cytogenet. Cell Genet. 57, 127–134.

    Article  PubMed  CAS  Google Scholar 

  • Iannuzzi, L., Di Meo, G.P., Perucatti, A., Ferrara, L. (1990). A comparison of G and R-banding in cattle and river buffalo prometaphase chromosomes. Caryologia 43, 283–290.

    Google Scholar 

  • Iannuzzi, L., Gallagher, D.S., Womack, J.E., Di Meo, G.P., Skow, L.C., Ferrara, L. (1993a). Chromosomal localization of the major histocompatibility complex in cattle and river buffalo by fluorescent in situ hybridization. Hereditas 118, 187–190.

    Article  PubMed  CAS  Google Scholar 

  • Iannuzzi, L., Gallagher, D.S., Ryan, A.M., Di Meo, G.P., Womack, J.E. (1993b). Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization. Cytogenet. Cell Genet. 62, 224–227.

    Article  PubMed  CAS  Google Scholar 

  • Iannuzzi, L., Di Meo, G.P., Ryan, A.M., Gallagher, D.S., Ferrara, L., Womack, J.E. (1994a). Localization of the bovine uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH. Chromosome Res. 2, 255–256.

    Article  PubMed  CAS  Google Scholar 

  • Iannuzzi, L., Gallagher, D.S., Liou, L.S., Di Meo, G.P., Ryan, A.M., Sastry, K.N., Womack, J.E. (1994b). Chromosomal localization of conglutinin (CGN1) gene to river buffalo by sequential RBH-banding and FISH. Hereditas 120, 283–286.

    Article  PubMed  CAS  Google Scholar 

  • Lalley, P.A., Davisson, M.T., Graves, J.A.M., O’Brien, S.J., Womack, J.E., Roderick, T.H., Creall-Goldberg, N., Hillard, A.L., Doolittle, D.P., Rogers, J.A. (1989). Report of the committee on comparative mapping. Cytogenet. Cell Genet. 51, 503–532.

    Article  PubMed  CAS  Google Scholar 

  • Li, L., Teale, A., Bensaid, A., Dunlap, S., Dietz, A.B., Womack, J.E. (1992). Somatic cell mapping of T-cell receptor CD3 complex and CD8 genes in cattle. Immunogenetics 36, 224–229.

    Article  PubMed  CAS  Google Scholar 

  • Miller, J.R., Thomsen, P.D., Dixon, S.C., Tucker, E.M., Konfortov, B.A., Harbitz, I. (1992). Synteny mapping of the bovine IGHG2, CRC and IGFI genes. Anim. Genet. 23, 51–58.

    PubMed  CAS  Google Scholar 

  • Neibergs, H.L., Gallagher, D.S., Georges, M., Sargeant, L.S., Dietz, A.B., Womack, J.E. (1993). Physical mapping of inhibin B-A in domestic cattle. Mamm. Genome 4, 328–332.

    Article  PubMed  CAS  Google Scholar 

  • Othman, O.E., Gallagher, D., de Hondt, H.A., El Nahas, S.M., Oraby, H.A., Bosma, A.A., Womack, J. (1994). Gene mapping in river buffalo: five syntenic groups. Proc. 11th European Colloquium on Cytogenetics of Domestic Animals, Frederiksberg, Denmark, Christensen, K., (ed) p. 193.

  • Report of the committee for the standardization of banded karyotypes of the river buffalo (1994). Standard karyotype of the river buffalo (Bubalis bubalis L., 2n = 50). Cytogenet. Cell Genet. 67, 102–113.

    Article  Google Scholar 

  • Ryan, A.M., Gallagher, D.S., Schober, S., Schwenger, B., Womack, J.E. (1994). Somatic cell mapping and in situ localization of the bovine uridine monophosphate synthase gene (UMPS). Mamm. Genome. 5, 46–47.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular cloning: a laboratory manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).

    Google Scholar 

  • Solinas Toldo, S., Mezzelani, A., Hawkins, G.A., Bishop, M.D., Olsaker, I., Mackinlay, A.G., Ferretti, L., Fries, R. (1995). Combined Q-banding and fluorescence in situ hybridization for the identification of bovine chromosomes 1 to 7. Cytogenet. Cell Genet. 69, 1–6.

    Article  PubMed  CAS  Google Scholar 

  • Southern, E.M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.

    Article  PubMed  CAS  Google Scholar 

  • Threadgill, D.W., Womack, J.E. (1990). Syntenic conservation between humans and cattle. I. Human chromosome 9. Genomics 8, 22–28.

    Article  PubMed  CAS  Google Scholar 

  • Threadgill, D.S., Threadgill, D.W., Moll, Y.D. Weiss, J.A., Zhang, N., Davey, H.W., Wildeman, A.G., Womack, J.E. (1994). Syntenic assignment of human chromosome 1 homologous loci in the bovine. Genomics 22, 626–630.

    Article  PubMed  CAS  Google Scholar 

  • Womack, J.E. (1987). Comparative gene mapping: a valuable new tool for mammalian developmental studies. Dev. Genet. 8, 281–293.

    Article  PubMed  CAS  Google Scholar 

  • Womack, J.E., Threadgill, D.W., Moll, Y.D., Faber, L.K., Foreman, M.L., Dietz, A.B., Tobin, T.C., Skow, L.K., Zneimer, S.M., Gallagher, D.S., Rogers, D.S. (1989). Syntenic mapping of 37 loci in cattle, chromosomal conservation with mouse and man. Cytogenet. Cell Genet. 51, 1109.

    Google Scholar 

  • Womack, J.E., Dietz, A.B., Gallagher, D.S., Li, L., Zhang, N., Neibergs, H.L., Moll, Y.D., Ryan, A.M. (1991). Assignment of 47 additional comparative anchor loci to the bovine synteny map. Cytogenet. Cell Genet. 58, 2132.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, National Research Center, Dokki, Cairo, Egypt

    S. M. El Nahas, H. A. Oraby, H. A. de Hondt, M. M. Zahran & E. R. Mahfouz

  2. Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

    A. M. Medhat & A. M. Karim

Authors
  1. S. M. El Nahas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. A. Oraby
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. A. de Hondt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. Medhat
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. M. Zahran
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. R. Mahfouz
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. M. Karim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

El Nahas, S.M., Oraby, H.A., de Hondt, H.A. et al. Synteny mapping in river buffalo. Mammalian Genome 7, 831–834 (1996). https://doi.org/10.1007/s003359900245

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s003359900245

Keywords

Search

Navigation