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Role of cAMP in Gibberellin Promotion of Seed Germination in Orobanche minor Smith

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Abstract

Adenosine 3′,5′-cyclic monophosphate (cAMP) is known as a key second messenger in many living organisms, regulating a wide range of cellular responses. In higher plants the function of cAMP is poorly understood. In this study, we examined the role of cAMP in seed germination of the root parasitic plant Orobanche minor whose seeds require preincubation in warm moist environments for several days, termed conditioning, prior to exposure to germination stimulants released from roots of host plants. Accumulation of endogenous cAMP was observed in the conditioned O. minor seeds. When the seeds were exposed to light or supraoptimal temperature during the conditioning period, cAMP did not accumulate and the seeds showed low germination rates after stimulation with strigol, a germination stimulant. Addition of membrane-permeable cAMP to the medium restored the germination rates of the seeds treated with light or supraoptimal temperature during the conditioning period, suggesting that cAMP functions during the conditioning period. The endogenous cAMP levels of the seeds conditioned in the light or at a supraoptimal temperature were elevated by treatment of the seeds with gibberellin (GA) during the conditioning period. Uniconazole, a potent inhibitor of GA biosynthesis, blocked elevation of the cAMP level. Furthermore, a correlation between the endogenous cAMP level and GA level was observed during the conditioning period. These results suggest that GAs elevate the cAMP level, which is required for the germination of O. minor seeds.

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Kimio Uematsu, Masatoshi Nakajima & Yasuhisa Fukui

  2. Maebashi Institute of Technology, Maebashi, Gunma, 371-0816, Japan

    Isomaro Yamaguchi

  3. Weed Science Center, Utsunomiya University, 350 Mine-machi, Utsunomiya, 321-8505, Japan

    Koichi Yoneyama

Authors
  1. Kimio Uematsu
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  2. Masatoshi Nakajima
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  3. Isomaro Yamaguchi
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  4. Koichi Yoneyama
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  5. Yasuhisa Fukui
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Correspondence to Yasuhisa Fukui.

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Uematsu, K., Nakajima, M., Yamaguchi, I. et al. Role of cAMP in Gibberellin Promotion of Seed Germination in Orobanche minor Smith. J Plant Growth Regul 26, 245–254 (2007). https://doi.org/10.1007/s00344-007-9012-9

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  • DOI: https://doi.org/10.1007/s00344-007-9012-9

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