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Red and Blue Light Emitting Diodes (LEDs) Participate in Mitigation of Hyperhydricity in In Vitro-Grown Carnation Genotypes (Dianthus Caryophyllus)

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Abstract

The present study was to determine the factors that can reduce hyperhydricity in in vitro-propagated carnation genotypes. The carnation genotypes (Green Beauty, Purple Beauty, and Inca Magic) were grown in vitro under normal and hyperhydric conditions in white fluorescent light (FL) in which half of the hyperhydric plants were grown in red and blue LEDs (light emitting diodes). It was observed that hyperhydricity leads to oxidative stress in terms of TBARS (thiobarbituric acid reactive substances) content, whereas stress was alleviated by R (red) and B (blue) LEDs. The multiprotein complex proteins such as ATPase (RCI + LHC1) PSII-core dimer, PSII-monomer/ATPs synthase, and PSII-monomer/cyt b6f had decreased levels in hyperhydric conditions grown in white FL; however, the expression level of these photosynthetic proteins was retained in hyperhydric plants grown in R and B LEDs. Moreover, the immunoblots of two photosynthetic proteins (PsaA and PsbA) and stress-responsive proteins such as superoxide dismutase, ascorbate peroxidase, and catalase showed recovery of hyperhydricity in carnation genotypes grown in R and B LEDs. Our present study signifies that red (R) and blue light (B) LEDs reduced the hyperhydricity to control levels by maintaining the composition of thylakoid proteins and antioxidative defense mechanisms in carnation genotypes.

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Acknowledgements

The authors would like to thank Prof. Euikyung Kim, College of Veterinary Medicine, Gyeongsang National University, Republic of Korea for sharing the BioRad ChemiDoc imaging system.

Funding

This work was supported from ‘Onsite Cooperative Agriculture Research’ (Project No. PJ01090805) RDA, Republic of Korea. SM was supported by BK21 Plus program (Brain Korea 21), Ministry of Education, Republic of Korea.

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Authors and Affiliations

  1. Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 52828, South Korea

    Sowbiya Muneer & Byoung Ryong Jeong

  2. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, South Korea

    Yoo Gyeong Park & Byoung Ryong Jeong

  3. Research Institute of Life Science, Gyeongsang National University, Jinju, 52828, South Korea

    Byoung Ryong Jeong

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  1. Sowbiya Muneer
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  2. Yoo Gyeong Park
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  3. Byoung Ryong Jeong
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Contributions

SM designed and performed the experiments; SM analyzed the data and wrote the manuscript; YG Park helped in maintaining the plant cultures; BRJ provided his lab for performing experiments.

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Correspondence to Byoung Ryong Jeong.

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Muneer, S., Park, Y.G. & Jeong, B.R. Red and Blue Light Emitting Diodes (LEDs) Participate in Mitigation of Hyperhydricity in In Vitro-Grown Carnation Genotypes (Dianthus Caryophyllus). J Plant Growth Regul 37, 370–379 (2018). https://doi.org/10.1007/s00344-017-9733-3

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