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Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster

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Abstract

Endogenous lectins act as effectors of cellular activities such as growth regulation, migration, and adhesion. Following their immunohistochemical localization in our previous study (Saussez et al. in Histochem Cell Biol 123:29–41, 2005) we purified several galectins and used them as tools for monitoring accessible binding sites. Herein, we report the use of galectin histochemistry for the analysis of diethylstilbestrol (DES)-induced renal tumors in male Syrian hamster kidney (SHKT). Sections of normal kidney and DES-treated kidney were analyzed with biotinylated galectins-1, -3 (full-length and truncated), and -7. Accessible binding sites were detected, localization was predominantly extracellular and confined to medium-sized and large tumors. Monitoring the SHKT-derived HKT-1097 line, processed in vitro or as xenograft material, cytoplasmic and nuclear staining for galectins-1, -3, and -3tr could be observed. Adaptation of SHKT cells to long-term growth in culture is thus associated with emergence of this signal. Our data set illustrates the feasibility to complement immunohistochemical data by application of the tissue lectins as probes, and to detect regulation of galectin reactivity with differential characteristics within tumor progression in vivo and unique features of the tumor cell line in vitro and in vivo.

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Abbreviations

BC:

Bowman’s capsule

DES:

Diethylstilbestrol

DMEM:

Dulbecco’s modified Eagle medium

DPBS:

Dulbecco’s PBS

ECM:

Extracellular matrix

FBS:

Fetal bovine serum

ISOM:

Inner stripe of the outer medulla

LI:

Labeling index

LT:

Large tumor

MOD:

Mean optical density

MT:

Medium-sized tumor

NGS:

Normal goat serum

OSOM:

Outer stripe of the outer medulla

PAF:

Paraformaldehyde

PAS:

Periodic acid-Schiff

PTAV:

Perivascular tissue surrounding arcuate artery and vein

RCC:

Renal cell carcinoma

s.c.:

Subcutaneous

SHKT:

Syrian hamster kidney tumor

TB:

Tumorous buds

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Acknowledgements

R. Kiss and G. Laurent are Research Director and Senior Research Associate, respectively, of the National Fund for Scientific Research (Belgium). G. Laurent is the recipient of a grant (no. 3.4512.03) from the Belgian Fund for Medical Scientific Research and H.-J. Gabius of grants from the Mizutani Foundation for Glycoscience (Tokyo, Japan) and the Verein zur Förderung des biologisch-technologischen Fortschritts in der Medizin e.V. (Heidelberg, Germany). The expert technical assistance of G. Ninfa is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Avenue du Champ de Mars, 6—Pentagone 1B, 7000, Mons, Belgium

    Sven Saussez, Francois Lorfevre, Denis Nonclercq, Guy Laurent & Gérard Toubeau

  2. Department of Anatomy, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Avenue du champ de Mars, 6—Pentagone 1B, 7000, Mons, Belgium

    Sven Saussez & Francois Lorfevre

  3. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, 80539, Munich, Germany

    Sabine André & Hans-Joachim Gabius

  4. Laboratory of Endocrinology/Bone Diseases and Department of Medicine, Institut Jules Bordet, Université Libre de Bruxelles, 1 Rue Héger-Bordet, 1000, Brussels, Belgium

    Fabrice Journé

  5. Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles, Campus Plaine CP 205/1, Blvd du Triomphe, 1050, Brussels, Belgium

    Robert Kiss

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  1. Sven Saussez
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  2. Francois Lorfevre
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Saussez, S., Lorfevre, F., Nonclercq, D. et al. Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem Cell Biol 126, 57–69 (2006). https://doi.org/10.1007/s00418-006-0146-9

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