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Adventitious rooting is enhanced by methyl jasmonate in tobacco thin cell layers

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Abstract

Adventitious roots (ARs) are induced by auxins. Jasmonic acid (JA) and methyl jasmonate (MeJA) are also plant growth regulators with many effects on development, but their role on ARs needs investigation. To this aim, we analyzed AR formation in tobacco thin cell layers (TCLs) cultured with 0.01–10 μM MeJA, either under root-inductive conditions, i.e., on medium containing 10 μM indole-3-butyric acid (IBA) and 0.1 μM kinetin, or without hormones. The explants were excised from the cultivars Samsun, Xanthii and Petite Havana, and from genotypes with altered AR-forming ability in response to auxin, namely the non-rooting rac mutant and the over-rooting Agrobacterium rhizogenes rolB transgenic line. Results show that NtRNR1 (G1/S) and Ntcyc29 (G2/M) gene activity, cell proliferation and meristemoid formation were stimulated in hormone-cultured TCLs by submicromolar MeJA concentrations. The meristemoids developed either into ARs and xylogenic nodules, or into xylogenic nodules only (rac TCLs). MeJA-induced meristemoid over-production characterized rolB TCLs. No rooting or xylogenesis occurred under hormone-free conditions, independently of MeJA and genotype. Endogenous JA progressively (days 1–4) increased in hormone-cultured TCLs in the absence of MeJA. JA levels were enhanced by 0.1 μM MeJA, on both days 1 and 4. Endogenous IBA was the only auxin detected, both in the free form and as IBA-glucose. Free IBA increased up to day 2, remaining constant thereafter (day 4). Its level was enhanced by 0.1 μM MeJA only on day 1, while IBA conjugation was not affected by MeJA. Taken together, these results show that an interplay between jasmonates and auxins regulates AR formation and xylogenesis in tobacco TCLs.

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Abbreviations

AR:

Adventitious root

HF:

Hormone free

IBA:

Indole-3-butyric acid

JA:

Jasmonic acid

Kin:

Kinetin

LR:

Lateral root

MCC:

Meristematic cell cluster

MeJA:

Methyl jasmonate

PR:

Primary root

SE:

Standard error

TCL:

Thin cell layer

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Acknowledgments

We thank Prof. Alessandra Gentili (Dipartimento di Chimica, Sapienza Università di Roma, Italy) for helpful suggestions in auxin detection, Prof. Stefania Biondi (Dipartimento di Biologia Evol. Sper., Università di Bologna, Italy) for helpful discussion on jasmonates, Dr. Marie-Edith Chabouté (Institut de Biologie Moléculaire des Plantes du CNRS, Universitè Louis Pasteur, Strasbourg, France) and Dr. Masami Sekine (Department of Bioproduction Science, Ishikawa Prefectural University, Ishikawa, Japan) for the generous gift of NtRNR1 and Ntcyc29 cDNA, respectively. We also thank Dr. Maura Cardarelli (Dipartimento di Genetica e Biologia Molecolare, Sapienza Università di Roma, Italy) for the generous gift of rolB seeds and for the preparation of the Ntcyc29 probe for in situ hybridizations. This work was supported by Progetto d’Ateneo, Sapienza Università di Roma (to MMA), and by Progetto di Ateneo Federato 2007–2008, Sapienza Università di Roma (to GF).

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Authors and Affiliations

  1. Dipartimento di Biologia Vegetale, Sapienza Università di Roma, P.le A. Moro 5, 00185, Rome, Italy

    Laura Fattorini, Giuseppina Falasca & Maria Maddalena Altamura

  2. Plant Biology Institute, University of Liège, Sart Tilman B22, 4000, Liege, Belgium

    Claire Kevers

  3. Dipartimento di Chimica, Sapienza Università di Roma, P.le A. Moro 5, 00185, Rome, Italy

    Lucia Mainero Rocca

  4. Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 72, 06121, Perugia, Italy

    Claudia Zadra

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  1. Laura Fattorini
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  2. Giuseppina Falasca
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  3. Claire Kevers
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  4. Lucia Mainero Rocca
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  6. Maria Maddalena Altamura
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Correspondence to Maria Maddalena Altamura.

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Fattorini, L., Falasca, G., Kevers, C. et al. Adventitious rooting is enhanced by methyl jasmonate in tobacco thin cell layers. Planta 231, 155–168 (2009). https://doi.org/10.1007/s00425-009-1035-y

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