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BTH and BABA induce resistance in pea against rust (Uromyces pisi) involving differential phytoalexin accumulation

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Abstract

Main Conclusion

Systemic acquired resistance elicitors, BTH and BABA, reduce rust penetration in pea through phytoalexins pathway but differing in their mode of action.

It has been previously shown that rust (Uromyces pisi) infection can be reduced in pea (Pisum sativum) by exogenous applications of systemic acquired resistance elicitors such as BTH and BABA. This protection is known to be related with the induction of the phenolic pathway but the particular metabolites involved have not been determined yet. In this work, we tackled the changes induced in phytoalexin content by BTH and BABA treatments in the context of the resistance responses to pea rust. Detailed analysis through high-performance liquid chromatography (HPLC) showed qualitative and quantitative differences in the content, as well as in the distribution of phytoalexins. Thus, following BTH treatment, we observed an increase in scopoletin, pisatin and medicarpin contents in all, excreted, soluble and cell wall-bound fraction. This suggests fungal growth impairment by both direct toxic effect as well as plant cell wall reinforcement. The response mediated by BTH was genotype-dependent, since coumarin accumulation was observed only in the resistant genotype whereas treatment by BABA primed phytoalexin accumulation in both genotypes equally. Exogenous application to the leaves of scopoletin, medicarpin and pisatin lead to a reduction of the different fungal growth stages, confirming a role for these phytoalexins in BTH- and BABA-induced resistance against U. pisi hampering pre- and postpenetration fungal stages.

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Abbreviations

BABA:

(D,L)-3-amino-n-butanoic ((D,L)-β-aminobutyric) acid

BTH:

Benzo[1,2,3]thiadiazole-7-carbothionic acid-S-methyl ester (Bion®)

SAR:

Systemic acquired resistance

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Acknowledgments

The authors are greatly indebted to the Spanish AGL2014-52871-R and Spanish-Italian HI2007-0233 projects for financial support. Dr. E. Barilli was granted by JAE-Doc program, co-financed by the European Social Foundation.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Córdoba, Spain

    Eleonora Barilli, Diego Rubiales & Elena Prats

  2. Agrarian Department, University of Naples Federico II, Portici, Italy

    Carmine Amalfitano

  3. Department of Chemical Science, University of Naples Federico II, Naples, Italy

    Antonio Evidente

Authors
  1. Eleonora Barilli
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  2. Diego Rubiales
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  3. Carmine Amalfitano
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  4. Antonio Evidente
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  5. Elena Prats
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Correspondence to Eleonora Barilli.

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Barilli, E., Rubiales, D., Amalfitano, C. et al. BTH and BABA induce resistance in pea against rust (Uromyces pisi) involving differential phytoalexin accumulation. Planta 242, 1095–1106 (2015). https://doi.org/10.1007/s00425-015-2339-8

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