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Dose-dependent response of Trichoderma harzianum in improving drought tolerance in rice genotypes

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Abstract

Main conclusion

This study demonstrates a dose-dependent response of Trichoderma harzianum Th-56 in improving drought tolerance in rice by modulating proline, SOD, lipid peroxidation product and DHN / AQU transcript level, and the growth attributes.

In the present study, the effect of colonization of different doses of T. harzianum Th-56 strain in rice genotypes were evaluated under drought stress. The rice genotypes treated with increasing dose of T. harzianum strain Th-56 showed better drought tolerance as compared with untreated control plant. There was significant change in malondialdehyde, proline, higher superoxide dismutase level, plant height, total dry matter, relative chlorophyll content, leaf rolling, leaf tip burn, and the number of scorched/senesced leaves in T. harzianum Th-56 treated rice genotypes under drought stress. This was corroborated with altered expression of aquaporin and dehydrin genes in T. harzianum Th-56 treated rice genotypes. The present findings suggest that a dose of 30 g/L was the most effective in improving drought tolerance in rice, and its potential exploitation will contribute to the advancement of rice genotypes to sustain crop productivity under drought stress. Interaction studies of T. harzianum with three aromatic rice genotypes suggested that PSD-17 was highly benefitted from T. harzianum colonization under drought stress.

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Abbreviations

AQP:

Aquaporin

DHN:

Dehydrin

MAD:

Malondialdehyde

SOD:

Superoxide dismutase

TDM:

The total dry matter

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Acknowledgments

The first author (V.P.) acknowledges the Department of Science and Technology, New Delhi for granting DST-INSPIRE fellowship for her Ph.D. programme. Authors are grateful to Department of Plant Physiology, Department of Plant Pathology and Department of Agronomy, G.B. Pant University of Agriculture and Technology Pantnagar for providing experimental facilities. The authors also wish to acknowledge All India Coordinated Rice Improvement Programme of ICAR and Indian Institute of Rice Research, Hyderabad for providing the necessary inputs for conducting the research work.

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    1. Department of Plant Physiology, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

      Veena Pandey, Gurdeep Bains, Shail Badal, Atul Kumar & Alok Shukla

    2. Department of Botany, Zakir Husain Delhi College, University of Delhi, Jawahar Lal Nehru Marg, New Delhi, 110002, India

      Mohammad W. Ansari & Narendra Tuteja

    3. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India

      Suresh Tula, Sandep Yadav, Ranjan K. Sahoo & Narendra Tuteja

    4. Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

      Nandini Shukla & J. Kumar

    5. Department of Agronomy, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

      Subhash Chandra

    6. Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

      A. K. Gaur

    7. Amity Institute of Microbial Technology, Amity University, E2-Block, 4th Floor, Room 404A, Sector 125, Noida, 201313, UP, India

      Narendra Tuteja

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    V. Pandey and M. W. Ansari contributed equally to this work.

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    Pandey, V., Ansari, M.W., Tula, S. et al. Dose-dependent response of Trichoderma harzianum in improving drought tolerance in rice genotypes. Planta 243, 1251–1264 (2016). https://doi.org/10.1007/s00425-016-2482-x

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