Skip to main content
SpringerLink
Log in

Clear cell sarcoma expressing a novel chimerical transcript EWSR1 exon 7/ATF1 exon 6

  • Case Report
  • Published:
Virchows Archiv Aims and scope Submit manuscript

Abstract

Clear cell sarcoma harbours recurrent translocation, resulting in EWSR1/ATF1 or less commonly EWSR1/CREB1 fusion. To date, six types of EWSR1/ATF1 fusion have been reported, of which three are in-frame and encode functional proteins. We present a reverse transcription - polymerase chain reaction analysis of a tumour near the hallux of the right foot. The sequencing of obtained fragments revealed the presence of a novel chimerical transcript—the in-frame fusion between EWSR1 exon 7 and ATF1 exon 6 that represents the fourth in-frame type of EWSR1/ATF1 fusion identified in clear cell sarcomas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Enzinger FM (1965) Clear-cell sarcoma of tendons and aponeuroses. An analysis of 21 cases. Cancer 18:1163–1174

    Article  PubMed  CAS  Google Scholar 

  2. Argani P, Perlman EJ, Breslow NE, Browning NG, Green DM, D'Angio GJ, Beckwith JB (2000) Clear cell sarcoma of the kidney: a review of 351 cases from the National Wilms Tumor Study Group Pathology Center. Am J Surg Pathol 24:4–18

    Article  PubMed  CAS  Google Scholar 

  3. Dim DC, Cooley LD, Miranda RN (2007) Clear cell sarcoma of tendons and aponeuroses: a review. Arch Pathol Lab Med 131:152–156

    PubMed  CAS  Google Scholar 

  4. WangW-L ME, Zhang W, Hernandez VS, Tuvin D, Garcia L, Lev DC, Lazar AJ, López-Terrada D (2009) Detection and characterization of EWSR1/ATF1 and EWSR1/CREB1 chimeric transcripts in clear cell sarcoma (melanoma of soft parts). Mod Pathol 22:1201–1209. doi:10.1038/modpathol.2009.85

    Article  Google Scholar 

  5. Jakubauskas A, Valceckiene V, Andrekute K, Seinin D, Kanopka A, Griskevicius L (2011) Discovery of two novel EWSR1/ATF1 transcripts in four chimerical transcripts-expressing clear cell sarcoma and their quantitative evaluation. Exp Mol Pathol 90:194–200. doi:10.1016/j.yexmp.2010.12.005

    Article  PubMed  CAS  Google Scholar 

  6. Panagopoulos I, Mertens F, Dêbiec-Rychter M, Isaksson M, Limon J, Kardas I, Domanski HA, Sciot R, Perek D, Crnalic S, Larsson O, Mandahl N (2002) Molecular genetic characterization of the EWS/ATF1 fusion gene in clear cell sarcoma of tendons and aponeuroses. Int J Cancer 99:560–567. doi:10.1002/ijc.10404

    Article  PubMed  CAS  Google Scholar 

  7. Fletcher CDM, Unni KK, Mertens F (eds) (2002) World Health Organization classification of tumours: pathology and genetics of tumours of soft tissue and bone. IARC, Lyon

    Google Scholar 

  8. Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL (2008) NCBI BLAST: a better web interface. Nucleic Acids Res 36:W5–W9. doi:10.1093/nar/gkn201

    Article  PubMed  CAS  Google Scholar 

  9. Bovee JV, Hogendoorn PC (2009) Molecular pathology of sarcomas: concepts and clinical implications. Virchows Arch 456:193–199

    Article  PubMed  Google Scholar 

  10. Yang K, Lui WO, Xie Y, Zhang A, Skytting B, Mandahl N, Larsson C, Larsson O (2002) Co-existence of SYT–SSX1 and SYT–SSX2 fusions in synovial sarcomas. Oncogene 21:4181–4190

    Article  PubMed  CAS  Google Scholar 

  11. Hurst HC (1995) Transcription factors 1: bZIP proteins. Protein Profile 2:101–168

    PubMed  CAS  Google Scholar 

Download references

Conflict of interest

We declare that we do not have conflict of interest.

Author information

Authors and Affiliations

  1. Hematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santariskiu Klinikos, Santariskiu 2, Vilnius, 08661, Lithuania

    Egle Gineikiene, Birute Brasiuniene, Laimonas Griskevicius & Arturas Jakubauskas

  2. National Center of Pathology, Vilnius University Hospital Santariskiu Klinikos, P. Baublio 5, Vilnius, 08406, Lithuania

    Dmitrij Seinin

  3. Center of Radiology, Vilnius University Hospital Santariskiu Klinikos, Santariskiu 2, Vilnius, 08661, Lithuania

    Andrius Brazaitis

  4. Clinics of Internal, Family Medicine and Oncology, Faculty of Medicine, Vilnius University, M.K. Ciurlionio 21, Vilnius, 03101, Lithuania

    Laimonas Griskevicius

Authors
  1. Egle Gineikiene
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dmitrij Seinin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Birute Brasiuniene
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrius Brazaitis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Laimonas Griskevicius
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Arturas Jakubauskas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arturas Jakubauskas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gineikiene, E., Seinin, D., Brasiuniene, B. et al. Clear cell sarcoma expressing a novel chimerical transcript EWSR1 exon 7/ATF1 exon 6 . Virchows Arch 461, 339–343 (2012). https://doi.org/10.1007/s00428-012-1295-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00428-012-1295-y

Keywords

Search

Navigation