Skip to main content

Advertisement

SpringerLink
Log in

Diagnosis of vivax malaria using an IgM capture ELISA is a sensitive method, even for low levels of parasitemia

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Although diagnosis of Plasmodium vivax malaria has been difficult when it is present at a low parasite density, it was recently revealed that an antibody assay was a good method of screening for malaria in blood banks. However, the use of this method for the diagnosis of malaria is limited due to the persistence of specific immunoglobulin (Ig) G. Therefore, we evaluated specific IgM antibody responses against the C-terminal region of the merozoite surface protein 1 of P. vivax (PvMSP1c) in sera obtained from patients with vivax malaria using various assays. The IgM capture enzyme-linked immunosorbent assay showed good sensitivity (97.7%; 308/315) and specificity (99.1%, 446/450). In addition, the results of this assay were not related to parasite density, and a high reactivity was observed when there was a low level of parasitemia. Furthermore, we found that patients with cases of malaria that had relapsed still had the IgM titers against PvMSP1c. Therefore, the use of IgM ELISA for the detection of specific IgM that was not involved in memorial immune activity could be an alternative tool for the diagnosis of malaria and blood screening, even in areas in which malaria is endemic.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Alves FP, Durlacher RR, Menezes MJ, Krieger H, Silva LH, Camargo EP (2002) High prevalence of asymptomatic Plasmodium vivax and Plasmodium falciparum infection in native Amazon populations. Am J Trop Med Hyg 66:641–648

    PubMed  Google Scholar 

  • Balmaseda A, Guzman MG, Hammond S, Robleto G, Flores C, Tellez Y, Videa E, Saborio S, Perez L, Sandoval E, Rodriguez Y, Harris E (2003) Diagnosis of dengue virus infection by detection of specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva. Clin Diagn Lab Immunol 10:317–322

    Article  PubMed  CAS  Google Scholar 

  • Branch OH, Udhayakumar V, Hightower AW, Oloo AJ, Hawley WA, Nahlen BL, Bloland PB, Kaslow DC, Lal AA (1998) A longitudinal investigation of IgG and IgM antibody responses to the merozoite surface protein-1 19-kiloDalton domain of Plasmodium falciparum in pregnant women and infants: associations with febrile illness, parasitemia, and anemia. Am J Trop Med Hyg 58:211–219

    PubMed  CAS  Google Scholar 

  • Cho D, Kim KH, Park SC, Kim YK, Lee KN, Lim CS (2001) Evaluation of rapid immunocapture assays for diagnosis of Plasmodium vivax in Korea. Parasitol Res 87:445–448

    Article  PubMed  CAS  Google Scholar 

  • Chung JY, Kim MY, Lim KJ, Sohn MJ, Kho WG (2001) Evaluation of LG Malaria Anti-Pvä for diagnosis of Plasmodium vivax malaria in the Republic of Korea. Korean J Clin Pathol 21:67–71

    Google Scholar 

  • Coleman RE, Maneechai N, Rachapaew N, Kumpitak C, Soyseng V, Miller RS, Thimasarn K, Sattabongkot J (2002) Field evaluation of the ICT Malaria Pf/Pv immunochromatographic test for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand. Am J Trop Med Hyg 66:379–383

    PubMed  Google Scholar 

  • Doderer C, Heschung A, Guntz P, Cazenave JP, Hansmann Y, Senegas A, Pfaff AW, Abdelrahman T, Candolfi E (2007) A new ELISA kit which uses a combination Plasmodium falciparum extract and Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies. Malaria J 6:1–8

    Article  CAS  Google Scholar 

  • Drakeley CJ, Corran PH, Coleman PG, Tongren JE, McDonald SLR, Malima R, Lusingu J, Manjurano A, Nkya WMM, Lemnge MM, Reyburn H, Riley EM (2005) Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure. Proc Natl Acad Sci 102:5108–5113

    Article  PubMed  CAS  Google Scholar 

  • Feighner BH, Pak SI, Novakoski WL, Kelsey LL, Strickman D (1998) Reemergence of Plasmodium vivax malaria in the Republic of Korea. Emerg Infect Dis 4:295–297

    Article  PubMed  CAS  Google Scholar 

  • Gaye O, Diouf M, Dansokho EF, McLaughlin G, Diallo S (1998) Diagnosis of Plasmodium falciparum malaria using ParaSight F, ICT malaria PF and malaria IgG Celisa assays. Parasite 5:189–192

    PubMed  CAS  Google Scholar 

  • Gutierrez A, Vicini J, Patarroyo ME, Murillo LA, Patarroyo MA (2000) Plasmodium vivax: Polymorphism in the merozoite surface proteins gene from wild Colombian isolates. Exp Parasitol 95:215–219

    Article  PubMed  CAS  Google Scholar 

  • Kaslow DC, Kumar S (1996) Expression and immunogenicity of C-terminus of major blood-stage surface protein of Plasmodium vivax, Pv20019, secreted from Saccharomyces cerevisiae. Immunol Letter 51:187–189

    Article  CAS  Google Scholar 

  • Korea National Red Cross (2001) Blood bank business annual report 2000. Korea National Red Cross, Seoul

    Google Scholar 

  • Lim KJ, Park JW, Sohn MJ, Lee S, Oh JH, Kim HC, Bahk YY, Kim YS (2002) A direct sandwich ELISA to detect antibodies against the C-terminal region of merozoite surface protein 1 could be a useful diagnostic method to identify Plasmodium vivax exposed persons. Parasitol Res 88:855–860

    Article  PubMed  Google Scholar 

  • Lim KJ, Park JW, Yeom JS, Lee YH, Yoo SB, Oh JH, Sohn MJ, Bahk YY, Kim YS (2004) Humoral responses against the C-terminal rejoin of merozoite surface protein 1 can be remembered for more than 30 years in persons exposed to Plasmodium vivax. Parasitol Res 92:384–389

    Article  PubMed  Google Scholar 

  • Mungai M, Tegtmeier G, Charmberland M, Parise M (2001) Transfusion-transmitted malaria in the United States from 1963 through 1999. N Eng J Med 344:1973–1978

    Article  CAS  Google Scholar 

  • Park JW, Moon SH, Yeom JS, Lim KJ, Sohn MJ, Jung WC, Cho YJ, Jeon KW, Ju W, Ki CS, Oh MD, Choe KW (2001) Naturally acquired antibody response to the C-terminal region of merozoite surface protein 1 of Plasmodium vivax in Korea. Clin Diagn Lab Immunol 8:14–20

    Article  PubMed  CAS  Google Scholar 

  • Ratnam S, Tipples G, Head C, Fauvel M, Fearon M, Ward BJ (2000) Performance of indirect immunoglobulin M (IgM) serology tests and IgM capture assay for laboratory diagnosis of measles. J Clin Microbiol 38:99–104

    PubMed  CAS  Google Scholar 

  • Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, Rosario VE, Thaithong V, Brown KN (1993) High sensitivity of detection of human malaria parasites by the use of nest polymerase chain reaction. Mol Biochem Parasitol 61:315–320

    Article  PubMed  CAS  Google Scholar 

  • Soares IS, Levitus G, Souza JM, Portillo HAD, Rodrigus MM (1997) Acquired immune response to N- and C-terminal region of Plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria. Infect Immun 65:1606–1614

    PubMed  CAS  Google Scholar 

  • Soares IS, da Cunha MG, Silva MN, Souza JM, Potillo HAD, Rodrigues MM (1999) Longevity of naturally acquired antibody response to the N- and C-terminal regions of Plasmodium vivax merozoite surface protein 1. Am J Trop Med Hyg 60:357–363

    PubMed  CAS  Google Scholar 

  • Udhayakumar V, Anyona D, Kariuki S, Shi YP, Bloland PB, Branch OH, Weiss W, Nahlen BL, Kaslow DC, Lal AA (1995) Identification of T and B cell epitopes recognized by humans in the C-terminal 42-kDa domain of the Plasmodium falciparum merozoite surface protein (MSP)-1. J Immunol 154:6022–6030

    PubMed  CAS  Google Scholar 

  • Wongsrichanalai C (2001) Rapid diagnostic techniques for malaria control. Trends in Parasitol 17:307–309

    Article  CAS  Google Scholar 

  • World Health Organization (2000) New perspectives malaria diagnosis. Report of a joint WHO/USAID informal consultation. WHO, Geneva

    Google Scholar 

  • Yeom JS, Kim TS, Oh S, Sim JB, Barn JS, Kim HJ, Kim YA, Ahn SY, Shin MY, Yoo JA, Park JW (2007) Plasmodium vivax malaria in the Republic of Korea during 2004–2005: changing patterns of infection. Am J Trop Med Hyg 76:865–868

    PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from the Korea Science and Engineering Foundation through the Protein Network Research Center at Yonsei University and through the National Core Research Center for Nanomedical Technology (R15-2004-024-00000-0).

Author information

Authors and Affiliations

  1. Department of Microbiology, Gachon Medical School, 405-760, Incheon, South Korea

    Jae-Won Park

  2. Diagnostics R&D Institute, LGLS Ltd., 104-1 Moonji-Dong, Yooseong-Gu, Taejon, 305-380, South Korea

    Seung Bum Yoo, Jae Hoon Oh & Kook Jin Lim

  3. Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 110-746, Seoul, South Korea

    Joon-Sup Yeom

  4. Department of Infection Biology, College of Medicine, Chungnam National University, 301-131, Taejon, South Korea

    Young-Ha Lee

  5. Department of Biochemistry, Protein Network Research Center, College of Science, Yonsei University, 120-749, Seoul, South Korea

    Young Yil Bahk & Yu Sam Kim

Authors
  1. Jae-Won Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seung Bum Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jae Hoon Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joon-Sup Yeom
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Young-Ha Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Young Yil Bahk
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yu Sam Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kook Jin Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kook Jin Lim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, JW., Yoo, S.B., Oh, J.H. et al. Diagnosis of vivax malaria using an IgM capture ELISA is a sensitive method, even for low levels of parasitemia. Parasitol Res 103, 625–631 (2008). https://doi.org/10.1007/s00436-008-1023-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-008-1023-3

Keywords

Search

Navigation