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Synthesis of pediculocidal and larvicidal silver nanoparticles by leaf extract from heartleaf moonseed plant, Tinospora cordifolia Miers

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Abstract

Insecticide resistance and inadequate attention to the application instructions of topical pediculicides are common reasons for treatment failure. Essential oils or plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. The present study was carried out to establish the pediculocidal and larvicidal activity of synthesized silver nanoparticles (AgNPs) using leaf aqueous extract of Tinospora cordifolia Miers (Menispermaceae) against the head louse Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae) and fourth instar larvae of malaria vector, Anopheles subpictus Grassi and filariasis vector, Culex quinquefasciatus Say (Diptera: Culicidae). We reported the aqueous plant extract and synthesized AgNPs against head lice and vectors. Direct contact method was conducted to determine the potential of pediculocidal activity. The synthesized AgNPs characterized by UV–vis spectrum, scanning electron microscopy, Fourier transform infrared, and X-ray diffraction. Head lice and mosquito larvae were exposed to varying concentrations of aqueous extracts and synthesized AgNPs for 24 h. The results suggest that the optimal times for measuring mortality effects of synthesized AgNPs were 33% at 5 min, 67% at 15 min, and 100% after 1 h. The maximum activity was observed in the synthesized AgNPs against lice, A. subpictus and C. quinquefasciatus (LC50 = 12.46, 6.43 and 6.96 mg/L; r 2 = 0.978, 0.773 and 0.828), respectively. The findings revealed that synthesized AgNPs possess excellent anti-lice and mosquito larvicidal activity. These results suggest that the green synthesis of AgNPs have the potential to be used as an ideal ecofriendly approach for the control of head lice and vectors.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  • Abdel-Ghaffar F, Semmler M (2007) Efficacy of neem seed extract shampoo on head lice of naturally infected humans in Egypt. Parasitol Res 100:329–332

    Article  PubMed  Google Scholar 

  • Abdel-Ghaffar F, Semmler M, Al-Rasheid K, Klimpel S, Mehlhorn H (2010a) Efficacy of a grapefruit extract on head lice: a clinical trial. Parasitol Res 106(2):445–449

    Article  PubMed  Google Scholar 

  • Abdel-Ghaffar F, Semmler M, Al-Rasheid K, Mehlhorn H (2010b) Comparative in vitro tests on the efficacy and safety of 13 anti-head lice products. Parasitol Res 106:423–429

    Article  PubMed  Google Scholar 

  • Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B 28:313–318

    Article  CAS  Google Scholar 

  • Amer A, Mehlhorn H (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res 99:478–490

    Article  PubMed  Google Scholar 

  • Anitha K, Shenoy RK (2001) Treatment of lymphatic filariasis: current trends. Indian J Dermatol Venereol Leprol 67(2):60–65

    PubMed  CAS  Google Scholar 

  • Anjali CH, Sudheer Khan S, Margulis-Goshen K, Magdassi S, Mukherjee A, Chandrasekaran N (2010) Formulation of water-dispersible nanopermethrin for larvicidal applications. Ecotoxicol Environ Saf 73(8):1932–1936

    Article  PubMed  CAS  Google Scholar 

  • Bansal SK, Singh KV, Kumar S (2009) Larvicidal activity of the extracts from different parts of the plant Solanum xanthocarpum against important mosquito vectors in the arid region. J Environ Biol 30(2):221–226

    PubMed  CAS  Google Scholar 

  • Baun A, Hartmann NB, Grieger K, Kusk KO (2008) Ecotoxicity of engineered nanoparticles to aquatic invertebrates: a brief review and recommendations for future toxicity testing. Ecotoxicology 17:387–396

    Article  PubMed  CAS  Google Scholar 

  • Begum NA, Mondal S, Basu S, Laskar RA, Mandal D (2009) Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts. Colloids Surf B Biointerfaces 71(1):113–118

    Article  PubMed  CAS  Google Scholar 

  • Bhattacharya SK (2001) A handbook of medicinal plants. Pointer, Jaipur, p 352

    Google Scholar 

  • Burgess IF, Brunton ER, Burgess NA (2010) Clinical trial showing superiority of a coconut and anise spray over permethrin 0.43% lotion for head louse infestation, ISRCTN96469780. Eur J Pediatr 169(1):55–62

    Article  PubMed  Google Scholar 

  • Carpinella MC, Miranda M, Almirón WR, Ferrayoli CG, Almeida FL, Palacios SM (2007) In vitro pediculicidal and ovicidal activity of an extract and oil from fruits of Melia azedarach L. J Am Acad Dermatol 56(2):250–256

    Article  PubMed  Google Scholar 

  • Chandra G, Bhattacharjee I, Chatterjee S (2010) A review on Anopheles subpictus Grassi—a biological vector. Acta Trop 15(2):142–154

    Article  Google Scholar 

  • Chatterjee SN, Chandra G (2000) Role of Anopheles subpictus as a primary vector of malaria in an area in India. Jpn J Trop Med Hyg 28(3):177–181

    Google Scholar 

  • Chauhan K (1995) Successful treatment of throat cancer with ayurvedic drugs. Suchitra Ayurved 47:840–842

    Google Scholar 

  • Crooks RM, Lemon BI, Sun L, Yeung LK, Zhao M (2001) Dendrimer-encapsulated metal nanoparticles: synthesis, characterization, and applications to catalysis. Top Curr Chem 212:82–135

    Google Scholar 

  • Dhanda V, Kaul HN (1980) Mosquito vectors of Japanese encephalitis virus and their bionomics in India. Proc Indian Natl Sci Acad 46B:759

    Google Scholar 

  • Dolianitis C, Sinclair R (2002) Optimal treatment of head lice: is a no-nit policy justified? Clin Dermatol 20:94–96

    Article  PubMed  Google Scholar 

  • Doucet M, Miranda M, Arce MA (1997) Prevalences of Pediculus humanus capitis and Sarcoptes scabiei in primary schools of Córdoba, Argentina. Analysis in relation to sex, age and socioeconomic conditions. Res Rev Parasitol 57:67–69

    Google Scholar 

  • Downs AM, Stafford KA, Coles GC (1999) Head lice: prevalence in school children and insecticide resistance. Parasitol Today 15:1–4

    Article  PubMed  CAS  Google Scholar 

  • Elechiguerra JL, Burt J, Morones JR, Camacho-Bragado A, Gao X, Lara HH, Yacaman MJ (2005) Interaction of silver nanoparticles with HIV-1. J Nanobiotechnol 3:6–12

    Article  Google Scholar 

  • Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, ISBN 052108041X. OCLC 174198382

    Google Scholar 

  • Gong P, Li H, He X, Wang K, Hu J, Tan W, Zhang S, Yang X (2007) Preparation and antibacterial activity of Fe3O4@Ag nanoparticles. Nanotechnology 18:285604

    Article  Google Scholar 

  • Gratz NG (1997) Human lice: their prevalence, control and resistance to insecticides—a review 1985Ð1997. World Health Organization, Geneva, Switzerland, WHO/CTD/WHOPES/97.8

  • Griffitt RJ, Luo J, Gao J, Bonzongo JC, Barber DS (2008) Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environ Toxicol Chem 27:1972–1978

    Article  PubMed  CAS  Google Scholar 

  • Hayes JB Jr, Laws ER Jr (1991) Handbook of pesticide toxicology, vol 1. Academic, San Diego

    Google Scholar 

  • Heukelbach J, Oliveira F, Speare R (2006a) A new shampoo based on neem (Azadirachta indica) is highly effective against head lice in vitro. Parasitol Res 99:353–356

    Article  PubMed  Google Scholar 

  • Heukelbach J, Speare R, Canyon D (2006b) Natural products and their application to the control of head lice: an evidence-based review. In: Brahmachari G (ed) Chemistry of natural products: recent trends and development. Kerala, India, pp 1–26

    Google Scholar 

  • Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    Article  PubMed  CAS  Google Scholar 

  • Kapura P, Pereiraa BMJ, Wattle W, Jarry H (2009) Androgenicaction of Tinospora cordifolia ethanolic extract in prostate cancer cell line LNCaP. Phytomedicine 16:679–682

    Article  Google Scholar 

  • Koodalingam A, Mullainadhan P, Arumugam M (2009) Antimosquito activity of aqueous kernel extract of soapnut Sapindus emarginatus: impact on various developmental stages of three vector mosquito species and nontarget aquatic insects. Parasitol Res 105(5):1425–1434

    Article  PubMed  Google Scholar 

  • Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B Biointerfaces 76(1):50–56

    Article  PubMed  CAS  Google Scholar 

  • Kulkarni SM (1983) Detection of sporozoites in Anopheles subpictus in Baster district, Madhya Pradesh. Indian J Malariol 20:159–160

    Google Scholar 

  • Mehlhorn B, Mehlhorn H (2009) Louse alarm. Düsseldorf University Press, Düsseldorf

    Google Scholar 

  • Mehlhorn H, Eichenlaub D, Löscher T, Peters W (1995) Diagnosis and therapy of human parasites. G. Fischer, 2. Aufl, Stuttgart

  • Michael E, Bundy DA, Grenfell BT (1996) Re-assessing the global prevalence and distribution of lymphatic filariasis. Parasitology 112(4):409–428

    Article  PubMed  Google Scholar 

  • Mouchet F, Landois P, Sarremejean E, Bernard G, Puech P, Pinelli E, Flahaut E, Gauthier L (2008) Characterisation and in vivo ecotoxicity evaluation of double-wall carbon nanotubes in larvae of the amphibian Xenopus laevis. Aquat Toxicol 87(2):127–137

    Article  PubMed  CAS  Google Scholar 

  • Mougabure Cueto G, Zerba E, Picollo MI (2008) Evidence of pyrethroid resistance in eggs of Pediculus humanus capitis (Phthiraptera: Pediculidae) from Argentina. J Med Entomol 45:693–697

    Article  Google Scholar 

  • Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800

    Article  CAS  Google Scholar 

  • Nagpal BN, Sharma VP (1995) Indian Anophelines. Oxford & IBH Pub Co. Pvt. Ltd, pp 189–190

  • Oladimeji FA, Orafidiya OO, Ogunniyi TA, Adewunmi TA (2000) Pediculocidal and scabicidal properties of Lippia multiflora essential oil. J Ethnopharmacol 72(2):305–311

    Article  PubMed  CAS  Google Scholar 

  • Panacek A, Kolar M, Vecerova R, Prucek R, Soukupova J, Krystof V, Hamal P, Zboril R, Kvitek L (2009) Antifungal activity of silver nanoparticles against Candida spp. Biomater 30:6333–6340

    Article  CAS  Google Scholar 

  • Panicker KN, GeethaBai M, Bheema Rao US, Viswam K, Suryanarayanamurthy U (1981) An. subpictus vector of malaria in coastal villages of South-East India. Curr Sci 50:694–695

    Google Scholar 

  • Parashar UK, Saxenaa PS, Srivastava A (2009) Bioinspired synthesis of silver nanoparticles. Dig J Nanomater Biostruct 4:159–166

    Google Scholar 

  • Picollo MI, Vassena C, Casadio A, Massimo J, Zerba E (1998) Laboratory studies of susceptibility and resistance to insecticides in Pediculus capitis (Anoplura: Pediculidae). J Med Entomol 35:814–817

    Google Scholar 

  • Picollo MI, Vassena C, Mougabure Cueto G, Vernetti M, Zerba E (2000) Resistance to insecticides and effect of synergists on permethrin toxicity in Pediculus capitis (Anoplura: Pediculidae) from Buenos Aires. J Med Entomol 37:721–725

    Google Scholar 

  • Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71(5):553–555

    Article  PubMed  CAS  Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83

    Article  PubMed  CAS  Google Scholar 

  • Ramaiah KD, Das PK, Michael E, Guyatt H (2000) The economic burden of lymphatic filariasis in India. Parasitol Today 16(6):251–253

    Article  PubMed  CAS  Google Scholar 

  • Rao TR (1984) The Anophelines of India. Malaria Research Centre (ICMR), Delhi, p 518

    Google Scholar 

  • Rao SK, Rao SP, Rao NB (2008) Preliminary investigation of the radiosensitizing activity of Guduchi (Tinospora cordifolia) in tumor bearing mice. Phytother Res 22:1482–1489

    Article  PubMed  Google Scholar 

  • Redwane A, Lazrek HB, Bouallam S, Markouk M, Amarouch H, Jana M (2002) Larvicidal activity of extracts from Quercus lusitania var. infectoria galls (Oliv.). J Ethnopharmacol 79:261–263

    Article  PubMed  CAS  Google Scholar 

  • Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM (2008) A preliminary assessment of silver nanoparticle inhibition of Monkeypox virus plaque formation. Nanoscale Res Lett 3:129–133

    Article  Google Scholar 

  • Rozendaal JA (1997) Methods for use by individuals and communities. World Health Organization, Geneva

    Google Scholar 

  • Sakulku U, Nuchuchua O, Uawongyart N, Puttipipatkhachorn S, Soottitantawat A, Ruktanonchai U (2009) Characterization and mosquito repellent activity of citronella oil nanoemulsion. Int J Pharm 8372(2):105–111

    Article  Google Scholar 

  • Santhoshkumar T, Rahuman AA, Rajakumar G, Marimuthu S, Bagavan A, Jayaseelan C, Zahir AA, Elango G, Kamaraj C (2010) Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res. doi:10.1007/s00436-010-2115-4

    Google Scholar 

  • Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces 73(2):332–338

    Article  PubMed  CAS  Google Scholar 

  • Schmahl G, Al-Rasheid KA, Abdel-Ghaffar F, Klimpel S, Mehlhorn H (2010) The efficacy of neem seed extracts (Tre-san, MiteStop) on a broad spectrum of pests and parasites. Parasitol Res 107(2):261–269

    Article  PubMed  Google Scholar 

  • Schultz S, Smith DR, Mock JJ, Schultz DA (2000) Single-target molecule detection with nonbleaching multicolor optical immunolabels. PNAS 97:996–1001

    Article  PubMed  CAS  Google Scholar 

  • Semmler M, Abdel-Ghaffar F, Al-Rasheid K, Mehlhorn H (2009) Nature helps: from research to products against blood-sucking arthropods. Parasitol Res. doi:10.1007/s00436-009-1634-3 (in press)

    Google Scholar 

  • Semmler M, Abdel-Ghaffar F, Al-Rasheid K, Klimpel S, Mehlhorn H (2010) Repellency against head lice (Pediculus humanus capitis). Parasitol Res 106(3):729–731

    Article  PubMed  Google Scholar 

  • Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M (2004) Biological synthesis of triangular gold nanoprisms. Nat Mater 3:482–488

    Article  PubMed  CAS  Google Scholar 

  • Shrivastava S, Dash D (2010) Label-free colorimetric estimation of proteins using nanoparticles of silver. Nano-Micro Lett 2:164–168

    CAS  Google Scholar 

  • Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC (2003) Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Indian J Pharmacol 35:83–91

    CAS  Google Scholar 

  • SPSS (2007) SPSS for Windows, version 16.0. Release 16.0.0 Chicago, IL, USA

  • Suber L, Sondi I, Matijevic E, Goia DV (2005) Preparation and the mechanisms of formation of silver particles of different morphologies in homogeneous solutions. J Colloid Interface Sci 288:489

    Article  PubMed  CAS  Google Scholar 

  • Sunilson JSAJ, Suraj R, Rejitha G, Anandarajagopal K, Vimala AG, Husain HA (2009) In vitro screening of anti-lice activity of Pongamia pinnata leaves. Korean J Parasitol 47(4):377–380

    Article  Google Scholar 

  • Thenmozhi V, Rajendran R, Ayanar K, Manavalan R, Tyagi BK (2006) Long-term study of Japanese encephalitis virus infection in Anopheles subpictus in Cuddalore district, Tamilnadu, South India. Trop Med Int Health 11(3):288–293

    Article  PubMed  CAS  Google Scholar 

  • Toloza AC, Zygadlo J, Cueto GM, Biurrun F, Zerba E, Picollo MI (2006) Fumigant and repellent properties of essential oils and component compounds against permethrin-resistant Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina. J Med Entomol 43(5):889–895

    Article  PubMed  CAS  Google Scholar 

  • Toloza A, Vassena C, Gallardo A, González-Audino P, Picollo MI (2009) Epidemiology of Pediculosis capitis in elementary schools of Buenos Aires, Argentina. Parasitol Res 104(6):1295–1298

    Article  PubMed  Google Scholar 

  • Toloza AC, Zygadlo J, Biurrun F, Rotman A, Picollo MI (2010a) Bioactivity of Argentinean essential oils against permethrin-resistant head lice, Pediculus humanus capitis. J Insect Sci 10:185

    Article  PubMed  Google Scholar 

  • Toloza AC, Lucía A, Zerba E, Masuh H, Picollo MI (2010b) Eucalyptus essential oil toxicity against permethrin-resistant Pediculus humanus capitis (Phthiraptera: Pediculidae). Parasitol Res 106(2):409–414

    Article  PubMed  Google Scholar 

  • Tripathi A, Chandrasekaran N, Raichur AM, Mukherjee A (2009) Antibacterial applications of silver nanoparticles synthesized by aqueous extract of Azadirachta indica (Neem) leaves. J Biomed Nanotechnol 5(1):93–98

    Article  PubMed  CAS  Google Scholar 

  • Vivekanandhan S, Misra M, Mohanty AK (2009) Biological synthesis of silver nanoparticles using Glycine max (soybean) leaf extract: an investigation on different soybean varieties. J Nanosci Nanotechnol 9(12):6828–6833

    PubMed  CAS  Google Scholar 

  • WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides. CTD/WHO PES/IC/96.1, 69

  • WHO (2005) Sixth meeting of the technical advisory group on the global elimination of lymphatic filariasis, Geneva, Switzerland. Wkly Epidemiol Rec 80:401–408

    Google Scholar 

  • Wiley BJ, Im SH, Li ZY, McLellan J, Siekkinen A, Xia Y (2006) Maneuvering the surface plasmon resonance of silver nanostructures through shape-controlled synthesis. J Phys Chem B 110(32):15666–15675

    Article  PubMed  CAS  Google Scholar 

  • Yang YC, Choi HY, Choi WS, Clark JM, Ahn YJ (2004) Ovicidal and adulticidal activity of Eucalyptus globulus leaf oil terpenoids against Pediculus humanus capitis (Anoplura: Pediculidae). J Agric Food Chem 52(9):2507–2511

    Article  PubMed  CAS  Google Scholar 

  • Zhang D, Qi L, Yang J, Ma J, Cheng H, Huang L (2004) Wet chemical synthesis of silver nanowire thin films at ambient temperature. Chem Mater 16:872

    Article  CAS  Google Scholar 

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  1. Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C.Abdul Hakeem College, Melvisharam, 632 509, Vellore District, Tamil Nadu, India

    Chidambaram Jayaseelan, Abdul Abdul Rahuman, Govindasamy Rajakumar, Arivarasan Vishnu Kirthi, Thirunavukkarasu Santhoshkumar, Sampath Marimuthu, Asokan Bagavan, Chinnaperumal Kamaraj, Abdul Abduz Zahir & Gandhi Elango

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  1. Chidambaram Jayaseelan
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Jayaseelan, C., Rahuman, A.A., Rajakumar, G. et al. Synthesis of pediculocidal and larvicidal silver nanoparticles by leaf extract from heartleaf moonseed plant, Tinospora cordifolia Miers. Parasitol Res 109, 185–194 (2011). https://doi.org/10.1007/s00436-010-2242-y

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