Skip to main content

Advertisement

SpringerLink
Log in

Larvicidal activity against Aedes aegypti of pacharin from Bauhinia acuruana

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The aim of the present study was to evaluate the activity of pacharin isolated from the ethanol extract from roots of Bauhinia acuruana on third-instar larvae of Aedes aegypti Linn. (Diptera: Culicidae). The crude ethanol extract showed larvicidal activity at the concentration of 500 μg/mL. Given this larvicidal activity, this extract was submitted to chromatographic fractionation on a silica gel column eluted with n-hexane, dichloromethane, ethyl ether, ethyl acetate, and methanol in order to isolate the active compound(s). Pacharin, obtained in pure form from fraction eluted with ethyl ether, was evaluated for their larvicidal effects against A. aegypti. In these bioassays, the larvae were exposed at concentrations of 500, 250, 100, 50, and 25 μg/mL of the crude ethanol extract or pacharin. After 24 h, the number of dead larvae was counted and the LC50 values for larval mortality were calculated. Pacharin showed LC50 value of 78.9 ± 1.8 μg/mL. The structure of isolated compound was identified on the basis of their spectral data (IR, 1D- and 2D-NMR) and by comparison with literature spectral data. The results indicate pacharin as a potential natural larvicide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Albuquerque MRJR, Silveira ER, Uchôa DEA, Lemos TLG, Souza EB, Santiago GMP, Pessoa ODL (2004) Chemical composition and larvicidal activity of the essential oils from Eupatorium betonicaeforme (D.C.) Baker (Asteraceae). J Agric Food Chem 52:6708–6711

    Article  PubMed  CAS  Google Scholar 

  • Anjaneyulu ASR, Raghava-Reddy AV, Reddy DSK, Ward RS, Adhikesavalu D, Stanley-Cameron T (1984) Pacharin: a new dibenzo(2,3-6,7)oxepin derivative from Bauhinia racemosa Lank. Tetrahedron 40:4245–4252

    Article  CAS  Google Scholar 

  • Araújo ECC, Silveira ER, Lima MAS, Neto MA, De Andrade IL, Lima MAA, Santiago GMP, Mesquita ALM (2003) Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth. J Agric Food Chem 51:3760–3762

    Article  PubMed  Google Scholar 

  • Chaton PF, Ravanel P, Tissut M, Meyran JC (2002) Toxicity and bioaccumulation of fipronil in the nontarget arthopodan fauna associated with subalpine mosquito breeding sites. Ecotoxicol Environ Saf 52:8–12

    Article  PubMed  CAS  Google Scholar 

  • Cechinel-Filho V (2009) Chemical composition and biological potential of plants from the genus Bauhinia. Phytother Res 23:1347–1354

    Article  PubMed  CAS  Google Scholar 

  • Feitosa EMA, Arriaga AMC, Santiago GMP, De Lemos TLG, De Oliveira MCF, Vasconcelos JN, Lima JQ, Malcher GT, Nascimento RF, Braz-Filho R (2009) Chemical composition and larvicidal activity of Rollinia leptopetala. J Braz Chem Soc 20:375–378

    Article  CAS  Google Scholar 

  • Finney DJ (1971) Probit analysis. Cambridge University Press, Cambridge

    Google Scholar 

  • Gois RWS, De Sousa LM, Lemos TLG, Arriaga AMC, Andrade-Neto M, Santiago GMP, Ferreira YS, Alves PB, De Jesus HCR (2011) Chemical composition and larvicidal effects of essential oil from Bauhinia acuruana (Moric) against Aedes aegypti. J Essent Oil Res 23:59–62

    Article  Google Scholar 

  • Grzybowski A, Tiboni M, Da Silva MAN, Chitolina RF, Passos M, Fontana JD (2012) The combined action of phytolarvicides for the control of dengue fever vector, Aedes aegypti. Rev Bras Farmacogn 22:549–557

    Article  CAS  Google Scholar 

  • Lomonaco D, Santiago GMP, Ferreira YS, Arriaga AMC, Mazzetto SE, Mele G, Vasapollo G (2009) Study of technical CNSL and its main components as new green larvicides. Green Chem 11:31–33

    Article  CAS  Google Scholar 

  • Macedo ME, Consoli RAGB, Grandi TSM, Dos Anjos AMG, De Oliveira AB, Mendes MN, Queiróz RO, Zani CL (1997) Screening of Asteraceae (Compositae) plants extracts for larvicidal activity against Aedes fluviatilis (Diptera: Culicidae). Mem Inst Oswaldo Cruz 92:565–570

    Article  PubMed  CAS  Google Scholar 

  • Macedo MLR, Freire MGM, Da Silva MBR, Coelho LCBB (2007) Insecticidal action of Bauhinia monandra leaf lectin (BmoLL) against Anagasta kuehniella (Lepidoptera: Pyralidae), Zabrotes subfasciatus and Callosobruchus maculatus (Coleoptera: Brucidae). Comp Biochem Phys A 146:486–498

    Article  Google Scholar 

  • Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M (2009) Larvicidal activity of Saraca indica, Nycthanthes arbor-tristis, and Clitorea ternatea extracts against three mosquito vector species. Parasitol Res 104:1017–1025

    Article  PubMed  Google Scholar 

  • Melo-Santos MAV, Varjal-Melo JJM, Araújo AP, Gomes TCS, Paiva MHS, Regis LN, Furtado AF, Magalhães T, Macoris MLG, Andrighetti MTM, Ayres CFJ (2010) Resistance to the organophosphate temephos: mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil. Acta Trop 113:180–189

    Article  PubMed  CAS  Google Scholar 

  • Mu L-H, Li J-B, Yang J-Z, Zhang D-M (2007) New dibenz[b,f]oxepins from Cercis chinensis Bunge. J Asian Nat Prod Res 9:649–653

    Article  PubMed  CAS  Google Scholar 

  • Oliveira MF, Lemos TLG, De Mattos MC, Segundo TA, Santiago GMP, Braz-Filho R (2002) New enamine derivatives of lapachol and biological activity. An Acad Bras Cienc 74:211–221

    Article  PubMed  CAS  Google Scholar 

  • Panneerselvam C, Murugan K, Kovendan K, Kumar PM (2012) Mosquito larvicidal, pupicidal, adulticidal, and repellent activity of Artemisia nilagirica (Family: Compositae) against Anopheles stephensi and Aedes aegypti. Parasitol Res 111:2241–2251

    Article  PubMed  Google Scholar 

  • Pettit GR, Numata A, Iwamoto C, Usami Y, Yamada T, Ohishi H, Cragg GM (2006) Antineoplasic agents. Isolation and structures of Bauhiniastatins from Bauhinia purpurea. J Nat Prod 69:323–327

    Article  PubMed  CAS  Google Scholar 

  • Polson KA, Brogdon WG, Rawlins SC, Chadee DD (2011) Characterization of insecticide resistance in Trinidadian strains of Aedes aegypti mosquitoes. Acta Trop 117:31–38

    Article  PubMed  CAS  Google Scholar 

  • Rajkumar S, Jebanesan A (2010) Chemical composition and larvicidal activity of leaf essential oil from Clausena dentata (Willd) M. Roam. (Rutaceae) against the chikungunya vector, Aedes aegypti Linn. (Diptera: Culicidae). J Asia Pac Entomol 13:107–109

    Article  CAS  Google Scholar 

  • Roth GN, Chandra A, Nair MG (1998) Novel bioactivities of Curcuma longa constituents. J Nat Prod 61:542–545

    Article  PubMed  CAS  Google Scholar 

  • Santos SRL, Melo MA, Cardoso AV, Santos RLC, De Sousa DP, Cavalcanti SCH (2011) Structure–activity relationships of larvicidal monoterpenes and derivatives against Aedes aegypti Linn. Chemosphere 84:150–153

    Article  PubMed  CAS  Google Scholar 

  • Santos GKN, Dutra KA, Barros RA, Da Câmara CAG, Lira DD, Gusmão NB, Navarro DMAF (2012) Essential oils from Alpinia purpurata (Zinziberaceae): chemical composition, oviposition deterrence, larvicidal and antibacterial activity. Ind Crop Prod 40:254–260

    Article  CAS  Google Scholar 

  • Schulze TL, Jordan RA, Hung RW, Krivenko AJ, Schulze JJ, Jordan TM (2001) Effects of an application of granular carbaryl on nontarget forest floor arthropods. J Econ Entomol 94:123–128

    Article  PubMed  CAS  Google Scholar 

  • Seo S-M, Park H-M, Park I-K (2012) Larvicidal activity of ajowan (Trachyspermun ammi) and Peru balsam (Myroxylon pereira) oils and blends of their constituents against mosquito, Aedes aegypti, acute toxicity on water flea, Daphnia magna, and aqueous residue. J Agric Food Chem 60:5909–5914

    Article  CAS  Google Scholar 

  • Sreelatha T, Hymavathi A, Murthy JM, Rani PU, Rao JM, Babu KS (2010) Bioactivity-guided isolation of mosquitocidal constituents from the rhizomes of Plumbago capensis Thunb. Bioorg Med Chem Lett 20:2974–2977

    Article  PubMed  CAS  Google Scholar 

  • Vasconcelos JN, Santiago GMP, Lima JQ, Mafezoli J, De Lemos TLG, Da Silva FRL, Lima MAS, Pimenta ATA, Braz-Filho R, Arriaga AMC, Cesarin-Sobrinho D (2012) Rotenoids from Tephrosia toxicaria with larvicidal activity against Aedes aegypti, the main vector of dengue fever. Quim Nova 35:1097–1100

    Article  Google Scholar 

  • Vaz AMST, Tozzi AMGA (2003) Bauhinia ser. Cansenia (Leguminosae: Caesalpinioideae) no Brasil. Rodriguésia 54:55–143

    Google Scholar 

  • Vimaladevi S, Mahesh A, Dhayanithi BN, Karthikeyan N (2012) Mosquito larvicidal efficacy of phenolic acids of seaweed Chaetomorpha antennina (Bory) Kuetz. against Aedes aegypti. Biologia 67:212–216

    Article  CAS  Google Scholar 

  • Waliwitiya R, Kennedy CJ, Lowenberger CA (2009) Larvicidal and ovipositon-altering activity of monoterpenoids, trans-anethole and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Manag Sci 65:241–248

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Huang E, Lin J, Gelbic I, Zhang Q, Guan Y, Huang T, Guan X (2010) A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudate. Microbiol Res 165:133–141

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are indebted to the Laboratório de Entomologia, Núcleo de Controle de Endemias Transmissíveis por Vetor-Secretaria de Saúde do Estado do Ceará, for providing the larvae and to the PRONEX, FUNCAP, FAPERJ, CAPES, and CNPq.

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Química, Universidade Federal do Ceará, 60451-970, Fortaleza-Ceará, Brazil

    Roberto Wagner da Silva Góis, Leôncio Mesquita de Sousa, Gilvandete Maria Pinheiro Santiago, Telma Leda Gomes Lemos & Angela Martha Campos Arriaga

  2. Departamento de Farmácia, Universidade Federal do Ceará, Rua Capitão Francisco Pedro 1210, 60430-370, Fortaleza-Ceará, Brazil

    Gilvandete Maria Pinheiro Santiago & Nirla Rodrigues Romero

  3. Setor de Química de Produtos Naturais, LCQUI-CCT, Universidade Estadual do Norte Fluminense, 28013-603, Campos-Rio de Janeiro, Brazil

    Raimundo Braz-Filho

Authors
  1. Roberto Wagner da Silva Góis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leôncio Mesquita de Sousa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gilvandete Maria Pinheiro Santiago
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nirla Rodrigues Romero
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Telma Leda Gomes Lemos
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Angela Martha Campos Arriaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Raimundo Braz-Filho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gilvandete Maria Pinheiro Santiago.

Rights and permissions

Reprints and permissions

About this article

Cite this article

da Silva Góis, R.W., de Sousa, L.M., Santiago, G.M.P. et al. Larvicidal activity against Aedes aegypti of pacharin from Bauhinia acuruana . Parasitol Res 112, 2753–2757 (2013). https://doi.org/10.1007/s00436-013-3407-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-013-3407-2

Keywords

Search

Navigation