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First report of Kudoa species in the somatic muscle of the Japanese parrotfish Calotomus japonicus (Scaridae) and a description of Kudoa igami , n. sp. (Myxozoa: Multivalvulida)

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Abstract

Three species of the Kudoid parasite (Myxozoa: Multivalvulida) were observed in the somatic muscle of Japanese parrotfish Calotomus japonicus caught off the coast of western Japan. All three species formed pseudocysts in myofibers and caused subclinical infections. The three Kudoa species were distinguished by spore morphology, as well as their 18S and 28S rDNA sequences. We identified a previously undescribed taxa Kudoa igami n. sp. with spores that were stellate with rounded peripheral edges and five to six polar capsules (prevalence 29.3 %). Kudoa igami n. sp. were morphologically most similar to Kudoa neothunni but were distinguishable by a more rounded shape in the apical view. Molecular analyses demonstrated that the K. igami n. sp. is closely related to Kudoa thalassomi; however, the similarity in the 28S rDNA sequence was <96 % and the spore morphology was different. We found Kudoa thalassomi in one sample (prevalence 2.4 %), which is a new host and geographical record for this species. Kudoa lateolabracis, which causes postmortem myoliquefaction in Chinese sea bass Lateolabrax sp. and olive flounder Paralichthys olivaceus was found in Japanese parrotfish (prevalence 41.5 %) for the first time, but did not cause myoliquefaction. We also expanded the host record for the brain-infecting Kudoa yasunagai (prevalence 94.1 %). In addition, an unidentified microsporidia was observed in the somatic muscle (prevalence 23.3 %).

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Acknowledgments

We would like to thank the Wakayama Prefectural Fisheries Experimental Station staff for storing the fish and helping with sampling. This research was supported in part by the SATREPS program of the Japan Science and Technology Agency (JST) to SS.

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Authors and Affiliations

  1. Fisheries Laboratory, Kinki University, Shirahama, Nishimuro, Wakayama, 649-2211, Japan

    Sho Shirakashi & Hiroe Ishitani

  2. Wakayama Prefectural Fisheries Experimental Station, Kushimoto, Higashimuro, Wakayama, 649-3503, Japan

    Koji Yamane

  3. Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, 078-8510, Japan

    Tetsuya Yanagida

  4. Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657, Japan

    Hiroshi Yokoyama

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  1. Sho Shirakashi
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  2. Koji Yamane
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  4. Tetsuya Yanagida
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  5. Hiroshi Yokoyama
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Correspondence to Sho Shirakashi.

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Shirakashi, S., Yamane, K., Ishitani, H. et al. First report of Kudoa species in the somatic muscle of the Japanese parrotfish Calotomus japonicus (Scaridae) and a description of Kudoa igami , n. sp. (Myxozoa: Multivalvulida). Parasitol Res 113, 2515–2524 (2014). https://doi.org/10.1007/s00436-014-3901-1

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  • DOI: https://doi.org/10.1007/s00436-014-3901-1

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