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β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production

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Abstract

β-Glucosidase (βG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.

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Acknowledgments

Part of this work was supported by the Korea Research Foundation (KRF) grant (No. 2009-0075638) funded by the Korean Ministry of Education, Science and Technology.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Inchon, 402-751, Korea

    Sang-Mok Lee & Yoon-Mo Koo

  2. Department of Chemical Engineering, Chosun University, Kwangju, 501-759, Korea

    Li Hua Jin & Jung-Heon Lee

  3. College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Sung Ok Han

  4. National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744, Korea

    Hyon Bin Na & Taeghwan Hyeon

  5. Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Korea

    Jae Hyun Kim & Jungbae Kim

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  1. Sang-Mok Lee
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  2. Li Hua Jin
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  7. Yoon-Mo Koo
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  9. Jung-Heon Lee
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Correspondence to Jungbae Kim or Jung-Heon Lee.

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Lee, SM., Jin, L.H., Kim, J.H. et al. β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production. Bioprocess Biosyst Eng 33, 141–147 (2010). https://doi.org/10.1007/s00449-009-0386-x

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  • DOI: https://doi.org/10.1007/s00449-009-0386-x

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