Abstract
The cultivation of microalgae in biofilm has been a potential way to overcome the shortcoming of conventional algal culture modes of open pond and photobioreactors in liquid suspension. However, the growth characteristics and related effect factors of the biofilm are still far from being understood. In this work, oleaginous microalgae species Pseudochlorococcum was cultured in an attached biofilm and influential factors on the growth rate of biofilm were investigated. The results showed that Pseudochlorococcum sp. preferred to accumulate more biomass on hydrophilic substrata than on hydrophobic one. The photon flux density of 100 μmol m−2 s−1 was its light saturation point. The optimal inoculum density was about 3–5 g m−2. The appropriate concentrations of nitrogen, phosphorus in medium and CO2 in aerated gas were determined as 8.8, 0.22 mmol L−1 and 1 %, respectively.
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References
Weisz PB (2004) Basic choices and constraints on long-term energy supplies. Phys Today 57:47–52
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Biofuels 25:294–306
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639
Wijffels RH, Barbosa MJ (2010) An outlook on microalgal biofuels. Science 330:913
Lam MK, Lee KT (2012) Microalgae biofuels: a critical review of issues, problems and the way forward. Biotechnol Adv 30:673–690
Larkum AWD, Ross IL, Kruse O, Hankame rB (2012) Selection, breeding and engineering of microalgae for bioenergy and biofuel production. Trends Biotechnol 30:198–205
Stephens E, Ross IL, Mussgnug JH, Wagner LD, Borowitzka MA, Posten C, Kruse O, Hankamer B (2010) Future prospects of microalgal biofuel production systems. Trends Plant Sci 15:554–564
Brennan L, Owende P (2010) Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products. Renew Sustain Energy Rev 14:557–577
Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev 14:217–232
Boyer JS (1982) Plant productivity and environment. Science 218:443–448
Tredici MR (2010) Photobiology of microalgae mass cultures: understanding the tools for the next green revolution. Biofuels 1:143–162
Zhu XG, Long SP, Ort DR (2008) What is the maximum efficiency with which photosynthesis can convert solar energy into biomass? Curr Opin Biotechnol 19:153–159
Liu T, Wang J, Hu Q, Cheng P, Ji B, Liu J, Chen Y, Zhang W, Chen X, Chen L, Gao L, Ji C, Wang H (2013) Attached cultivation technology of microalgae for efficient biomass feedstock production. Bioresour Technol 127:216–222
Cheng P, Ji B, Gao L, Zhang W, Wang J, Liu T (2013) The growth, lipid and hydrocarbon production of Botryococcus braunii with attached cultivation. Bioresour Technol 138:95–100
Shi J, Podola B, Melkonian M (2007) Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study. J Appl Phycol 9:417–423
Mulbry W, Kangas P, Kondrad S (2010) Toward scrubbing the bay: nutrient removal using small algal turf scrubbers on Chesapeake Bay tributaries. Ecol Eng 36:536–541
Boelee NC, Temmink H, Janssen M, Buisman CJN, Wijffels RH (2011) Nitrogen and phosphorus removal from municipal wastewater effluent using microalgal biofilms. Water Res 45:5925–5933
Naumann T, Cebi Z, Podola B, Melkonian M (2013) Growing microalgae as aquaculture feeds on twin-layers: a novel solid-state photobioreactor. J Appl Phycol 5:1413–1420
Zamalloa C, Boon N, Verstraete W (2013) Decentralized two-stage sewage treatment by chemical–biological flocculation combined with microalgae biofilm for nutrient immobilization in a roof installed parallel plate reactor. Bioresour Technol 30:152–160
Johnson MB, Wen ZY (2010) Development of an attached microalgal growth system for biofuel production. Appl Microbiol Biotechnol 5:525–534
Ozkan A, Kinney K, Katz L, Berberoglu H (2012) Reduction of water and energy requirement of algae cultivation using an algae biofilm photobioreactor. Bioresour Technol 114:542–548
Largeau C, Casadevall E, Berkaloff C, Dhamelincourt P (1980) Sites of accumulation and composition of hydrocarbons in Botryococcus braunii. Phytochemistry 19:1043–1051
Richmond A, Zhang CW, Zarmi Y (2003) Efficient use of strong light for high photosynthetic productivity: interrelationships between the optical path, the optimal population density and cell-growth inhibition. Biomol Eng 20:229–236
Cui Y, Yuan W (2013) Thermodynamic modeling of algal cell–solid substrate interactions. Appl Energy 112:485–492
Shen Y, Xu X, Zhao Y, Lin X (2013) Influence of algae species, substrata and culture conditions on attached microalgal culture. Bioprocess Biosyst Eng. doi:10.1007/s00449-013-1011-6
Ji C, Wang J, Zhang W, Liu J, Wang H, Gao L, Liu T (2013) An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus. J Appl Phycol. doi:10.1007/s10811-10013-10115-10813
Li YT, Han DX, Sommerfeld M, Hu Q (2011) Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions. Bioresour Technol 102:123–129
Acknowledgments
This work was supported by the Key Technologies R&D Program from Ministry of Science and Technology of China (2011BAD14B01), the Solar Energy Initiative Plan (KGCX2-EW-309) from Chinese Academy of Sciences, and National Natural Science Foundation of China (41276144).
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Ji, B., Zhang, W., Zhang, N. et al. Biofilm cultivation of the oleaginous microalgae Pseudochlorococcum sp.. Bioprocess Biosyst Eng 37, 1369–1375 (2014). https://doi.org/10.1007/s00449-013-1109-x
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DOI: https://doi.org/10.1007/s00449-013-1109-x