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Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.

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Abstract

Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. This review describes and compares the efficiency of different conventional and novel techniques that can be used for cell disruption and recovery of bio-molecules from Nannochloropsis sp. Classification of different extraction techniques includes chemical, enzymatic, mechanical and other physical methods. The detailed analysis of extraction efficiency assisted by pressure and temperature (subcritical and supercritical fluids, hydrothermal liquefaction), ultrasound, microwaves, and pulsed electric energy (pulsed electric fields and high voltage electrical discharges) is presented. The general discussion includes comparison between techniques, their effectiveness for cell disruption and selectivity of bio-molecules extraction from Nannochloropsis sp. The cost-effectiveness, benefits and limitations of different techniques are also analyzed.

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Acknowledgements

Rui Zhang would like to acknowledge the financial support of China Scholarship Council for thesis fellowship.

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Authors and Affiliations

  1. Laboratoire de Transformations Intégrées de la Matière Renouvelable, EA 4297, Centre de Recherches de Royallieu, Sorbonne Universités, Université de Technologie de Compiègne, BP 20529, 60205, Compiègne Cedex, France

    Rui Zhang, Oleksii Parniakov, Nabil Grimi, Nikolai Lebovka & Eugène Vorobiev

  2. Institute of Biocolloidal Chemistry named after F. D. Ovcharenko, NAS of Ukraine, 42, blvr. Vernadskogo, Kyiv, 03142, Ukraine

    Nikolai Lebovka

  3. LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l’Université, BP 406, 44602, Saint-Nazaire Cedex, France

    Luc Marchal

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  1. Rui Zhang
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  2. Oleksii Parniakov
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  3. Nabil Grimi
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  4. Nikolai Lebovka
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  5. Luc Marchal
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  6. Eugène Vorobiev
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Correspondence to Rui Zhang.

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Zhang, R., Parniakov, O., Grimi, N. et al. Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.. Bioprocess Biosyst Eng 42, 173–186 (2019). https://doi.org/10.1007/s00449-018-2038-5

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