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Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment

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Abstract

This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, including Marinobacter hydrocarbonoclaustics and Marinobacter alkaliphilus. Electrochemical analysis such as impedance and polarization results indicated that Proteobacteria biofilm accelerated corrosion by ~ twofold (2.1 ± 0.61 mm/years) or ~ fourfold (~ 3.7 ± 0.42 mm/years) when compared to the control treatment (0.95 ± 0.1 mm/years). Scanning electron and atomic force microscopy revealed the presence of a thick biofilm and pitting corrosion. X-ray diffraction revealed higher amounts of the corrosion products Fe2O3, γ-FeOOH, and α-FeOOH, and confirmed that the microbial biofilm strongly oxidized the iron and contributed to the acceleration of corrosion of carbon metal API 5LX.

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Acknowledgements

The authors thank DST-SERB (Proj. File.No. EEQ/2016/000449). The authors are grateful to the Researchers Supporting Project Number (RSP-2020/68), King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. School of Environment, South China Normal University, University Town, Guangzhou, 510006, Guandong, China

    Punniyakotti Elumalai

  2. Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box-2455, Riyadh, 11451, Kingdom of Saudi Arabia

    Mohamad S. AlSalhi & Sandhanasamy Devanesan

  3. Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa Dell’Annunziata, Via Roma 29, 81031, Aversa, CE, Italy

    Sanjeet Mehariya

  4. College of Technology, University of Houston, Houston, TX, 77204, USA

    Obulisamy Parthiba Karthikeyan

  5. Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India

    Punniyakotti Parthipan

  6. Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore, 632115, Tamil Nadu, India

    Aruliah Rajasekar

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Elumalai, P., AlSalhi, M.S., Mehariya, S. et al. Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment. Bioprocess Biosyst Eng 44, 355–368 (2021). https://doi.org/10.1007/s00449-020-02447-w

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