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Micropropagation of axillary shoots of Salix viminalis using a temporary immersion system

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Culturing axillary shoot explants of willow in liquid medium in plantform™ and RITA ® vessels increased the multiplication coefficient relative to that obtained by culturing the explants in semisolid medium in jars.

Abstract

A protocol for micropropagating willow by multiplying axillary shoots in liquid medium was developed, and the effects of the type of explant, medium composition, bioreactor type and frequency of immersion were investigated. The type of explant influenced shoot quality and the multiplication coefficient, with basal segments showing a higher capacity for proliferation than apical and mid-shoot segments. Hyperhydricity was not observed in shoots cultured with Murashige and Skoog medium with half-strength nitrates and supplemented with 0.22 µM BA and 3% sucrose. The willow shoots cultured in plantform™ and RITA® vessels yielded higher multiplication coefficients than shoots grown in semisolid medium. High proliferation rates were obtained by immersion of shoots for 1 min three or six times a day, with additional aeration of 1 min per hour in the plantform™ bioreactors. Transfer to medium with 1% sucrose after 4 weeks of culture in medium with 3% sucrose increased the multiplication coefficient of basal segments cultured in RITA® vessels, suggesting that the species could be micropropagated under photoautotrophic conditions. The willow shoots rooted spontaneously and were successfully acclimatized to greenhouse conditions. This is the first report of the use of a temporary immersion system to culture Salix viminalis. The study findings indicate the feasibility of the system as a method for the large-scale propagation of this species.

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Acknowledgements

We dedicate this article to the memory of our dear colleague and friend, Brais Bogo Graña, who died before this research was completed. We thank Estiben Becerra, Alejandro Díaz, Rafael Sánchez and Patricia Val for technical assistance. This research was partially funded by the Xunta de Galicia (Spain) through the Contrato Programa 2014–2016.

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  1. Brais Bogo: deceased.

Authors and Affiliations

  1. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Apdo 122, 15780, Santiago de Compostela, Spain

    María Regueira, Eva Rial, Blandina Blanco, Brais Bogo, Anxela Aldrey, Begoña Correa, Elena Varas, Conchi Sánchez & Nieves Vidal

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  1. María Regueira
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  2. Eva Rial
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Correspondence to Nieves Vidal.

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Regueira, M., Rial, E., Blanco, B. et al. Micropropagation of axillary shoots of Salix viminalis using a temporary immersion system. Trees 32, 61–71 (2018). https://doi.org/10.1007/s00468-017-1611-x

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