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The co-occurrence of ectomycorrhizal, arbuscular mycorrhizal, and dark septate fungi in seedlings of four members of the Pinaceae

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Abstract

Although roots of species in the Pinaceae are usually colonized by ectomycorrhizal (EM) fungi, there are increasing reports of the presence of arbuscular mycorrhizal (AM) and dark septate endophytic (DSE) fungi in these species. The objective of this study was to determine the colonization patterns in seedlings of three Pinus (pine) species (Pinus banksiana, Pinus strobus, Pinus contorta) and Picea glauca x Picea engelmannii (hybrid spruce) grown in soil collected from a disturbed forest site. Seedlings of all three pine species and hybrid spruce became colonized by EM, AM, and DSE fungi. The dominant EM morphotype belonged to the E-strain category; limited colonization by a Tuber sp. was found on roots of Pinus strobus and an unknown morphotype (cf. Suillus–Rhizopogon group) with thick, cottony white mycelium was present on short roots of all species. The three fungal categories tended to occupy different niches in a single root system. No correlation was found between the percent root colonized by EM and percent colonization by either AM or DSE, although there was a positive correlation between percent root length colonized by AM and DSE. Hyphae and vesicles were the only AM intracellular structures found in roots of all species; arbuscules were not observed in any roots.

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Acknowledgements

We thank the Natural Sciences and Engineering Research Council of Canada for financial support in the form of a Discovery grant to RLP and an Undergraduate Summer Fellowship to MP. We thank Forrest Phillips for help with the plate and Linda Tackaberry for comments on the manuscript. We thank Dr. Scott Green, UNBC, for the hybrid spruce seeds.

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada

    Cameron Wagg, Michael Pautler & R. Larry Peterson

  2. Ecosystem Science and Management Program, University of Northern British Columbia, 3333 University Way, Prince George, British Columbia, V2N 4Z9, Canada

    Hugues B. Massicotte

Authors
  1. Cameron Wagg
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  2. Michael Pautler
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  3. Hugues B. Massicotte
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  4. R. Larry Peterson
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Correspondence to R. Larry Peterson.

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Wagg, C., Pautler, M., Massicotte, H.B. et al. The co-occurrence of ectomycorrhizal, arbuscular mycorrhizal, and dark septate fungi in seedlings of four members of the Pinaceae. Mycorrhiza 18, 103–110 (2008). https://doi.org/10.1007/s00572-007-0157-y

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  • DOI: https://doi.org/10.1007/s00572-007-0157-y

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