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Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

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Abstract

The seed germination of orchids under natural conditions requires association with mycorrhizal fungi. Dendrobium nobile and Dendrobium chrysanthum are threatened orchid species in China where they are considered medicinal plants. For conservation and application of Dendrobium using symbiosis technology, we isolated culturable endophytic and mycorrhizal fungi colonized in the protocorms and adult roots of two species plants and identified them by morphological and molecular analyses (5.8S and nrLSU). Of the 127 endophytic fungi isolated, 11 Rhizoctonia-like strains were identified as Tulasnellales (three strains from protocorms of D. nobile), Sebacinales (three strains from roots of D. nobile and two strains from protocorms of D. chrysanthum) and Cantharellales (three strains from roots of D. nobile), respectively. In addition, species of Xylaria, Fusarium, Trichoderma, Colletotrichum, Pestalotiopsis, and Phomopsis were the predominant non-mycorrhizal fungi isolated, and their probable ecological roles in the Dendrobium plants are discussed. These fungal resources will be of great importance for the large-scale cultivation of Dendrobium plants using symbiotic germination technology and for the screening of bioactive metabolites from them in the future.

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Acknowledgments

This investigation was supported by the National Natural Science Foundation of China (30900004, 30770068), National High Technology Research and Development Program of China (2008AA09Z405), and National S & T Major Special Project on Major New Drug Innovation (2009ZX09301-003-3-3).

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, People’s Republic of China

    Juan Chen, Hui Wang & Shun-Xing Guo

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  1. Juan Chen
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  2. Hui Wang
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  3. Shun-Xing Guo
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Correspondence to Shun-Xing Guo.

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Chen, J., Wang, H. & Guo, SX. Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae). Mycorrhiza 22, 297–307 (2012). https://doi.org/10.1007/s00572-011-0404-0

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  • DOI: https://doi.org/10.1007/s00572-011-0404-0

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