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Arbuscular mycorrhizal fungi from New Caledonian ultramafic soils improve tolerance to nickel of endemic plant species

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Abstract

In order to improve knowledge about the role of arbuscular mycorrhizal fungi (AMF) in the tolerance to heavy metals in ultramafic soils, the present study investigated the influence of two Glomus etunicatum isolates from New Caledonian ultramafic maquis (shrubland), on nickel tolerance of a model plant species Sorghum vulgare, and of two ultramafic endemic plant species, Alphitonia neocaledonica and Cloezia artensis. In a first step, plants were grown in a greenhouse, on sand with defined concentrations of Ni, to appreciate the effects of the two isolates on the alleviation of Ni toxicity in controlled conditions. In a second step, the influence of the AMF on A. neocaledonica and C. artensis plants grown in a New Caledonian ultramafic soil rich in extractable nickel was investigated. Ni reduced mycorrhizal colonization and sporulation of the fungal isolates, but the symbionts increased plant growth and adaptation of endemic plant species to ultramafic conditions. One of the two G. etunicatum isolates showed a stronger positive effect on plant biomass and phosphorus uptake, and a greater reduction in toxicity symptoms and Ni concentration in roots and shoots. The symbionts seemed to act as a barrier to the absorption of Ni by the plant and reduced root-to-shoot Ni translocation. Results indicate the potential of selected native AMF isolates from ultramafic areas for ecological restoration of such degraded ecosystems.

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Acknowledgments

This study was funded by two projects of “Agence Nationale de la Recherche Scientifique”: “ANR ECCO” and “ANR Ultrabio” and also by BioTop project CNRT “Nickel et son Environnement”. We want to thank Valérie Médevielle, Armelle Renard, Cindy Pupier, and Xavier Cuniberti for their contribution to the technical support of the study.

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Authors and Affiliations

  1. Laboratoire Insulaire du Vivant et de l’Environnement (LIVE), Université de la Nouvelle-Calédonie, BP R4-98851 Nouméa Cedex, Nouvelle-Calédonie, France

    Hamid Amir, Alexandre Lagrange & Yvon Cavaloc

  2. NH Consultant, New Caledonia, France

    Nadine Hassaïne

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  1. Hamid Amir
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  2. Alexandre Lagrange
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  3. Nadine Hassaïne
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  4. Yvon Cavaloc
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Correspondence to Hamid Amir.

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Amir, H., Lagrange, A., Hassaïne, N. et al. Arbuscular mycorrhizal fungi from New Caledonian ultramafic soils improve tolerance to nickel of endemic plant species. Mycorrhiza 23, 585–595 (2013). https://doi.org/10.1007/s00572-013-0499-6

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  • DOI: https://doi.org/10.1007/s00572-013-0499-6

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