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Effect of sex on haemocytobiochemical profiling of silver tiger fish (Datnioides polota Hamilton, 1822)

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Abstract

This investigation presents an overview of the effect of sex on haematobiochemical and haemocytomorphometrical aspects of Datnioides polota. For the present study, 15 males and 15 females of Datnioides polota were collected from Balugaon, the central sector of Chilika lagoon of Odisha. Two millilitres of blood samples was collected from each sex through the caudal vein. One millilitre of collected blood was transferred from the syringe into ethylenediaminetetraacetic acid (EDTA) containing vials, for haematological studies and other 1 ml was taken in Eppendorf tube without anticoagulant for analyses of biochemical parameters. Haematological parameters like red blood cell count (RBC), white blood cells count (WBC), haemoglobin, haematocrit, mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), biochemical parameters such as glucose, protein, albumin, globulin, cholesterol, and morphometrical parameters namely length, breadth, area, and N/C ratio of erythrocytes, lymphocytes, monocytes, eosinophils, and neutrophils are analysed in relation to the sex of the fish. Statistical analysis reveals the sex wise significant difference in some haematological parameters like Hb which varies at P < 0.01 level while WBC deviate at P < 0.05 level. All the biochemical parameters show higher value in females in comparison to males except albumin but sex wise significant variation recorded in protein and globulin at P < 0.01 level. In males, the concentration of Hb is positively correlated with RBC and PCV while in females negatively correlated. RBC is positively correlated with PCV in both the sexes at R2 = 0.008 and R2 = 0.9 in male and female, respectively. The morphometry of blood cells such as monocytes and lymphocytes shows significant sexual dimorphism. The cellular length and area of monocytes deviate at the level of P < 0.01 and breadth at P < 0.05 level. The cellular and nuclear breadth of lymphocyte vary significantly at the level of P < 0.05 and P < 0.001, respectively. The results of this study may serve as a reference value for haemocytological and biochemical parameters of this species which in turn helps in diagnosing diseases and increasing fish production.

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Funding

This study was funded by the Department of Science and Technology, New Delhi, India (grant number IF 130 457).

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Authors and Affiliations

  1. PG Department of Zoology, Research Scholar, Utkal University, Vani Vihar, Bhubaneswar, India

    Gayatri Acharya

  2. PG Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar, India

    Prafulla Kumar Mohanty

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  1. Gayatri Acharya
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  2. Prafulla Kumar Mohanty
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Correspondence to Gayatri Acharya.

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This investigation followed all the guidelines for the care and use of animals.

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Acharya, G., Mohanty, P.K. Effect of sex on haemocytobiochemical profiling of silver tiger fish (Datnioides polota Hamilton, 1822). Comp Clin Pathol 27, 1335–1342 (2018). https://doi.org/10.1007/s00580-018-2744-z

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