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Fast detection of catechin in tea beverage using a poly-aspartic acid film based sensor

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Abstract

A fast and convenient analytical method is presented for the determination of catechin. The electrochemical response of catechin in pH 6.8 phosphate buffer solution is significantly enhanced by immobilization of a film of poly-aspartic acid on the surface of the glassy carbon electrode. The enhancement mechanism and effect factors such as pH value, accumulation time and scan rate, were explored. Under optimum conditions, the differential pulse voltammetry peak current of catechin is proportional to the concentration in the range from 2.5 × 10−7 to 3.0 × 10−5 molL−1, with the detection limit of 7.2 × 10−8 molL−1. This method was also applied to the determination of catechin in tea beverage samples, and the recoveries were from 97.1% to 102.7%.

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Acknowledgements

This work was supported by the Natural Science Foundation of Shanghai (No: 09ZR1434100) and the Nano-Foundation of Shanghai (No: 0952nm00800).

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Authors and Affiliations

  1. Department of Chemistry, Tongji University, Shanghai, 200092, Peoples Republic of China

    Xiao-Gang Wang & Jing Li

  2. Department of Maths, Tongji University, Shanghai, 200092, Peoples Republic of China

    Ya-Juan Fan

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  1. Xiao-Gang Wang
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  2. Jing Li
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  3. Ya-Juan Fan
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Correspondence to Xiao-Gang Wang.

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Wang, XG., Li, J. & Fan, YJ. Fast detection of catechin in tea beverage using a poly-aspartic acid film based sensor. Microchim Acta 169, 173–179 (2010). https://doi.org/10.1007/s00604-010-0335-z

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  • DOI: https://doi.org/10.1007/s00604-010-0335-z

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