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Amperometric nonenzymatic determination of glucose based on a glassy carbon electrode modified with nickel(II) oxides and graphene

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Abstract

We have developed a stable and sensitive nonenzymatic glucose sensor by modifying a glassy carbon electrode (GCE) with a composite incorporating nickel(II) oxides and reduced graphene. The oxides were generated by directly electrodepositing nickel on the GCE with a graphene modifier using a multi-potential pulse process, and then oxidizing nickel to nickel(II) oxides by potential cycling. In comparison to the conventional nickel(II) oxides-modified GCE, this new nickel(II) oxides-graphene modified GCE (NiO-GR/GCE) has an about 1.5 times larger current response toward the nonenzymatic oxidation of glucose in alkaline media. The response to glucose is linear in the 20 μM to 4.5 mM concentration range. The limit of detection is 5 μM (at a S/N of 3), and the response time is very short (<3 s). Other beneficial features include selectivity, reproducibility and stability. A comparison was performed on the determination of glucose in commercial red wines by high-performance liquid chromatography (HPLC) and revealed the promising aspects of this sensor with respect to the determination of glucose in real samples.

A stable and sensitive nonenzymatic glucose sensor is developed by preparing the nickel(II) oxides-reduced graphene nanocomposite modified glassy carbon electrode (NiO-GR/GCE), and then used to detect the glucose contents in the commercial red wines. This NiO-GR/GCE also has a high selectivity

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21175047 and 21147003) and the Scientific Research Foundation of Graduate School of South China Normal University (2012kyjj217).

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Authors and Affiliations

  1. School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, People’s Republic of China

    Xiaohua Zhu, Qifang Jiao, Chuyi Zhang, Xiaoxi Zuo, Xin Xiao, Yong Liang & Junmin Nan

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  1. Xiaohua Zhu
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  2. Qifang Jiao
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  3. Chuyi Zhang
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  4. Xiaoxi Zuo
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  5. Xin Xiao
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  6. Yong Liang
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  7. Junmin Nan
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Correspondence to Junmin Nan.

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Zhu, X., Jiao, Q., Zhang, C. et al. Amperometric nonenzymatic determination of glucose based on a glassy carbon electrode modified with nickel(II) oxides and graphene. Microchim Acta 180, 477–483 (2013). https://doi.org/10.1007/s00604-013-0955-1

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  • DOI: https://doi.org/10.1007/s00604-013-0955-1

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