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Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

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Abstract

The authors describe the preparation of desert rose-like gold nanoparticles (DR-GNPs) with a plasmon resonance band at 620 nm which gives them a blue color. They have a hydrodynamic diameter of ∼72 nm and were prepared by a seeding growth approach. The DR-GNPs were characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. These nonspherical GNPs were used as a label for the antibody in an immunochromatographic strip test (ICST). Despite their particular shape and the higher surface area compared to spherical gold nanoparticles, the DR-GNPs are useful blue labels for the GNP-based strip test. A multicolor ICST for aflatoxin B1 and fumonisins is described that employs both blue DR-GNPs and red spherical GNPs. It allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels of 2 μg⋅kg-1 for aflatoxin B1 and of 1000 μg⋅kg-1 for fumonisins.

Blue desert rose-like gold nanoparticles (DR-GNPs) were synthesized, characterized and applied as label for the ImmunoChromatographic Strip Test (ICST) technique, in which red spherical GNPs (s-GNPs) are usually employed. The combined use of the blue DR-GNP and red s-GNPs allowed developing of an intuitive multicolor ICST for the simultaneous detection of aflatoxin B1 and fumonisins in maize flour.

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Acknowledgements

Authors are grateful to Dr. M. Manzoli and Prof. V. Maurino for the fruitful and helpful discussion related to the TEM and DLS measurements.

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Authors and Affiliations

  1. Department of Chemistry, University of Turin, Via Giuria, 5, I-10125, Turin, Italy

    Fabio Di Nardo, Claudio Baggiani, Cristina Giovannoli, Giulia Spano & Laura Anfossi

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  1. Fabio Di Nardo
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  2. Claudio Baggiani
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  3. Cristina Giovannoli
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  4. Giulia Spano
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  5. Laura Anfossi
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Correspondence to Laura Anfossi.

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Di Nardo, F., Baggiani, C., Giovannoli, C. et al. Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins. Microchim Acta 184, 1295–1304 (2017). https://doi.org/10.1007/s00604-017-2121-7

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