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Amperometric determination of organophosphate pesticides using a acetylcholinesterase based biosensor made from nitrogen-doped porous carbon deposited on a boron-doped diamond electrode

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Abstract

The authors describe an amperometric biosensor for the determination of organophosphate pesticides (OPs) via inhibition of the enzyme acetylcholinesterase (AChE). The enzyme was immobilized on nitrogen-doped porous carbon and then placed on boron-doped diamond (BDD). The Michaelis-Menten constant for immobilized AChE is 0.177 mmol L−1, indicating that AChE has a stronger enzymatic activity and affinity due to the introduction of nitrogen-doped porous carbon. The biosensor was applied to the determination of the OPs dichlorvos and fenitrothion. The efficiency of the inhibition by dichlorvos increases linearly in the 100 pg·L-1 to 10.0 μg·L-1 concentration range. The detection limit is as low as 1.50 pg·L-1. The inhibition by fenitrothion can be detected in the same concentration range, and the detection limit is 4.40 pg·L-1 (calculated for 10% inhibition). The unique sensitivity of this assay make it a most attractive tool for the detection of OPs.

Nitrogen-doped porous carbon is used to develop an AChE/biosensor based on a boron-doped diamond electrode. The biosensor shows higher sensitivity, lower detection limit, good reproducibility and acceptable stability towards organophosphate pesticides detection. (AChE: acetylcholinesterase; BDD: boron-doped diamond)

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Acknowledgements

This research was supported by the Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology (2016RCJH04).

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  1. College of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001, People’s Republic of China

    Min Wei & Shuo Feng

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  1. Min Wei
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  2. Shuo Feng
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Correspondence to Min Wei.

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Wei, M., Feng, S. Amperometric determination of organophosphate pesticides using a acetylcholinesterase based biosensor made from nitrogen-doped porous carbon deposited on a boron-doped diamond electrode. Microchim Acta 184, 3461–3468 (2017). https://doi.org/10.1007/s00604-017-2380-3

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  • DOI: https://doi.org/10.1007/s00604-017-2380-3

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