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Etched PtCu nanowires as a peroxidase mimic for colorimetric determination of hydrogen peroxide

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Abstract

Etched PtCu nanowires (NWs) were synthesized by a hydrothermal reaction and chemical etching process. The NWs are shown to be viable peroxidase (POx) mimics capable of catalyzing the oxidation of the substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to form a blue-green coloration. The mechanism of catalysis was investigated and the results demonstrated that H2O2 is decomposed to form hydroxyl radicals which oxidize TMB in the presence of the NWs. Under optimized conditions, a steady-state kinetic analysis revealed that the NWs possess a stronger affinity for H2O2 and TMB compared to the enzyme horseradish POx. Based on the high POx-like activity, a colorimetric assay for H2O2 was established. Absorbance at 652 nm increases linearly in the 0.1–300 μM H2O2 concentration range, and the detection limit is 0.06 μM (at S/N = 3). The assay was successfully applied to the determination of H2O2 in (spiked) milk and contact lens solution. Furthermore, a highly sensitive test strip was designed which represents a low cost and fast alternative for the visual determination of H2O2.

Schematic presentation of the colorimetric detection of H2O2. PtCu nanowires (PtCu NWs) can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) oxidation by H2O2 to produce blue-green oxidized 3,3',5,5'-tetramethylbenzidine (oxTMB). Based on the color change, test strips were designed for H2O2 detection.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21501106), the Natural Science Foundation of Shandong Province (JQ201704), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Qingdao Municipal Science and Technology Commission (16-5-1-86-jch), and the Institutional Development Award (P20GM103424).

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Authors and Affiliations

  1. College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Ning Sui, Shuai Li, Yukai Wang, Qiang Bai, Hailian Xiao, Manhong Liu & William W. Yu

  2. Department of Chemistry, Brown University, Providence, RI, 02912, USA

    Qingbo Zhang

  3. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Shufeng Liu

  4. College of Environment and Safety Engineering, Key Laboratory of Eco-Chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao, 266042, China

    Lina Wang

  5. Department of Chemistry and Physics, Louisiana State University, Shreveport, LA, 71115, USA

    William W. Yu

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  1. Ning Sui
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Sui, N., Li, S., Wang, Y. et al. Etched PtCu nanowires as a peroxidase mimic for colorimetric determination of hydrogen peroxide. Microchim Acta 186, 186 (2019). https://doi.org/10.1007/s00604-019-3293-0

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