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Closing the gaps: phylogenetic relationships in the Brassicaceae based on DNA sequence data of nuclear ribosomal ITS region

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Abstract

Sequence data from the nuclear encoded ribosomal internal transcribed spacer (ITS) region were used to determine monophyly of tribes, tribal limits, and tribal relationships of 96 so far unassigned or tentatively assigned genera (represented by 101 taxa/accessions) within the Brassicaceae. Maximum-parsimony and maximum-likelihood analyses of 185 ITS Brassicaceae sequences, which also included representatives of each of the 34 currently recognized tribes, supported the separate phylogenetic distinctness of these tribes and permitted the tribal assignment of all but 12 of the unassigned genera into tribal clades. The data support the recognition of eight new, well-resolved, uni- or oligogeneric tribes recognized herein as the Alyssopsideae [96% bootstrap support (BS); including the central and southwestern Asian Alyssopsis and Calymmatium], Asteae (100% BS; including the Mexican Asta), Eudemeae (97% BS; South American Brayopsis, Eudema, and Xerodraba), Kernereae (96% BS; European Kernera and Rhizobotrya), Notothlaspideae (100% BS; New Zealandic Notothlaspi), Oreophytoneae (100% BS; eastern African Oreophyton and southern European Murbeckiella), and Yinshanieae (100% BS; Chinese Yinshania), as well as the moderately supported Microlepidieae (75% BS; Australian Microlepidium and Carinavalva). Furthermore, the results fully support the recent findings that the tribes Schizopetaleae and Thelypodieae ought to be recognized as two distinct tribes instead of a single tribe, as well as provide some support for the re-establishment of the tribe Cremolobeae, bringing the total number to 44 tribes in the family. Nearly 92% (308) of the 336 genera in the family have been assigned to a tribe. The earlier-published Anastaticeae is taken here to replace the Malcolmieae.

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Acknowledgments

Fieldwork by Al-Shehbaz in South America was supported by National Geographic Society grant 8139-06. We wish to thank Dr. Mark Beilstein, The Arnold Arboretum Harvard University, for providing two unpublished ITS sequences, and Ulrike Coja for technical assistance.

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Authors and Affiliations

  1. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Central Experimental Farm, Ottawa, ON, K1A 0C6, Canada

    Suzanne I. Warwick & Connie A. Sauder

  2. FB Biologie/Chemie, Botanik, Universität Osnabrück, 49069, Barbarastrasse II, Germany

    Klaus Mummenhoff

  3. Heidelberg Institute of Plant Sciences, Biodiversity and Plant Systematics, University of Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany

    Marcus A. Koch

  4. Missouri Botanical Garden, P.O. Box 299, St. Louis, MO, 63166-0299, USA

    Ihsan A. Al-Shehbaz

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  1. Suzanne I. Warwick
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  2. Klaus Mummenhoff
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  3. Connie A. Sauder
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  4. Marcus A. Koch
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  5. Ihsan A. Al-Shehbaz
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Correspondence to Suzanne I. Warwick.

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Warwick, S.I., Mummenhoff, K., Sauder, C.A. et al. Closing the gaps: phylogenetic relationships in the Brassicaceae based on DNA sequence data of nuclear ribosomal ITS region. Plant Syst Evol 285, 209–232 (2010). https://doi.org/10.1007/s00606-010-0271-8

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  • DOI: https://doi.org/10.1007/s00606-010-0271-8

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