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Post-translational regulation of rotavirus protein NSP1 expression in mammalian cells

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Summary.

The nonstructural rotavirus protein NSP1 binds specifically to viral mRNAs and to interferon regulatory factor 3 (IRF3), inducing IRF3 degradation through a proteasome-dependent pathway. By using a vaccinia virus expression system in mammalian cells, we found that the yield of NSP1 was 8- and 13-fold lower than the viral proteins VP2 or NSP3, respectively; while in the presence of proteasome inhibitors such difference could be reduced to 2- to 2.5-fold, respectively. The susceptibility of NSP1 to proteasome degradation was fully reversed in a dose-dependent manner by transfection with the full complement of 11 molecules of translation-competent rotavirus mRNAs, but this effect was abrogated by the protein synthesis inhibitor cycloheximide. These results demonstrate that NSP1 is degraded through a proteasome-dependent pathway, and viral proteins, alone or in combination with viral mRNAs, interfere with such degradation.

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  1. Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico

    C. Piña-Vázquez, M. De Nova-Ocampo, S. Guzmán-León & L. Padilla-Noriega

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  1. C. Piña-Vázquez
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  2. M. De Nova-Ocampo
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  3. S. Guzmán-León
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  4. L. Padilla-Noriega
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Piña-Vázquez, C., De Nova-Ocampo, M., Guzmán-León, S. et al. Post-translational regulation of rotavirus protein NSP1 expression in mammalian cells. Arch Virol 152, 345–368 (2007). https://doi.org/10.1007/s00705-006-0850-8

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  • DOI: https://doi.org/10.1007/s00705-006-0850-8

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