Abstract
In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Damayanti TA, Susilo D, Nurlaelah S, Sartiami D, Okuno T, Mise K (2008) First report of Bean common mosaic virus in yam bean [Pachyrhizus erosus (L.) Urban] in Indonesia. J Gen Plant Pathol 74:438–442
FAO (1979) Review on agriculture and development. Conventional crops imperil good protein. Ceres 12:4–5
Ha C, Revill P, Harding RM, Vu M, Dale JI (2008) Identification and sequence analysis of potyviruses infecting crops in Vietnam. Arch Virol 153:45–60
King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2011) Virus taxonomy: classification and nomenclature of viruses. Ninth report of the international committee on the taxonomy of viruses. Elsevier Academic Press, San Diego
Kreuze JF, Perez A, Untiveros M, Quispe D, Fuentes S, Barker I, Simon R (2009) Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: a generic method for diagnosis, discovery and sequencing of viruses. Virology 388:1–7
NRC (1979) Tropical legumes. Resources for the future. National Academy of Science. NRC National Research Council, Washington
Sørensen M (1996) Yam bean (Pachyrhizus DC.) Promoting the conservation and use of underutilized and neglected crops. 2. Institute of Plant Genetics and Crop Plant Research (IPK), International Plant Genetic Resources Institute (IPGRI), Gatersleben, Germany
Acknowledgments
We would like to thank Mirella Flores for help with bioinformatics.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fuentes, S., Heider, B., Tasso, R.C. et al. Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru. Arch Virol 157, 773–776 (2012). https://doi.org/10.1007/s00705-011-1214-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-011-1214-6