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Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells

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Abstract

Chikungunya virus (CHIKV), the virus responsible for the disease chikungunya fever in humans, is transmitted by Aedes mosquitoes. While significant progress has been made in understanding the process by which CHIKV enters into mammalian cells, far less progress has been made in understanding the CHIKV entry process in insect cells. This study sought to identify mosquito-cell-expressed CHIKV-binding proteins through a combination of virus overlay protein binding assays (VOPBA) and mass spectroscopy. A 50-kDa CHIKV-binding protein was identified as the ATP synthase β subunit (ATPSβ). Co-immunoprecipitation studies confirmed the interaction, and colocalization analysis showed cell-surface and intracellular co-localization between CHIKV and ATPSβ. Both antibody inhibition and siRNA-mediated downregulation experiments targeted to ATPSβ showed a significant reduction in viral entry and virus production. These results suggest that ATPSβ is a CHIKV-binding protein capable of mediating the entry of CHIKV into insect cells.

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Acknowledgments

This work was supported by grants from the Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative and Mahidol University. C.F. is supported by a Royal Golden Jubilee Scholarship, P.W. is supported by a Thailand Graduate Institute of Science and Technology (TGIST) Ph.D. Scholarship, and N.W. is supported by a TRF and Mahidol University (Thai Royal Golden Jubilee) PhD Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand

    Chanida Fongsaran, Krit Jirakanwisal, Atichat Kuadkitkan, Nitwara Wikan, Phitchayapak Wintachai, Chutima Thepparit & Duncan R. Smith

  2. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand

    Sukathida Ubol

  3. Center for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Thailand

    Sukathida Ubol & Duncan R. Smith

  4. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand

    Narumon Phaonakrop & Sittiruk Roytrakul

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  1. Chanida Fongsaran
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Correspondence to Duncan R. Smith.

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Fongsaran, C., Jirakanwisal, K., Kuadkitkan, A. et al. Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells. Arch Virol 159, 3353–3364 (2014). https://doi.org/10.1007/s00705-014-2210-4

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  • DOI: https://doi.org/10.1007/s00705-014-2210-4

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